A Microfluidic Device with Groove Patterns for Studying Cellular Behavior

0:06

Introduction

1:27

Microfabrication of microfluidic device

1:45

Preparation of PDMS mixture

2:42

Patterning PDMS molds

3:36

Removing bubbles by vacuum

4:21

Assembly of microfluidic device

4:55

Cutting layers of microfluidic device

6:10

Bonding the microfluidic device layers

8:19

Coating channels with fibronectin

9:08

Experiment: monitoring apoptosis

9:09

Cell seeding

11:31

Application of reagents

13:32

Time lapse of apoptosis