Lo studio è stato sostenuto dalla Fondazione artères, la Astellas Foundation Europe e dalla Fondazione Boninchi. CT è stato sostenuto dal Fondo nazionale svizzero (SCORE concessione 3.232.230-126.233).

1. Maniglia: Progettazione e Shaping <ol><li> Fissare un Nr. 10 lama di bisturi di carbonio su una tavola di legno con un nastro. </li><li> Segnare il centro dell&#39;anello al microscopio. La posizione deve essere 1,2 centimetri rette dal boschetto della lama del bisturi. </li><li> Praticare un foro di 2 mm con un po diamante sferica, montato su un trapano ad alta velocità. </li><li> Allargare il foro con fusiforme o coniche punte di diamante. Il diametro finale varia a seconda del peso ratto come mostrato nella Tabella 1. </li><li> Progettare la forma del manico con un pennarello indelebile. Figure 1 e 2 possono essere utilizzati come matrici. </li><li> Convertire il trapano in un mini smerigliatrice e rimuovere la parte di lama all&#39;esterno della forma. </li><li> Concludete il pezzo con una bassa velocità spazzola rotante. </li></ol> 2. Approssimatore: Progettazione e Shaping <ol><li> Il approssimatore è ottenuto modificando dritto chirurgica del Klemmer o forcep di Kochers. </li><li> Tagliare la metà di un ramo sotto il microscopio con il mini banco smerigliatrice ad alta velocità. </li><li> Fai gole simmetriche su entrambi i lati per consentire la maniglia per far scorrere in tutto. </li><li> Tagliare una fessura longitudinale nel braccio lungo e controllare che il centro del braccio lungo e una maniglia sono ben posizionata su un medesimo asse. </li><li> Progettare una tacca sul braccio lungo rivolto verso il centro del manico. </li><li> Fai il ramo più sottile possibile e completano il pezzo con la bassa velocità spazzola rotante. </li></ol> 3. Polsini: Progettazione e Shaping <ol><li> Pinze Cuff variano in base al peso di ratto e di deformazione. Per contro, la loro lunghezza deve essere tenuto circa 4 mm. </li><li> Prendere fine-alesaggio tubo di polietilene e completano un bordo con una Nr. 10 bisturi. </li><li> Progettare un boschetto di 0,5 mm dal bordo, e tagliare con precisione un pendio che scende verso il boschetto. </li><li> Tagliare una o due tacche a tutto spessore nel tubo. Questi sarannocontribuire a manipolare il bracciale durante la preparazione del trapianto e trapianto. </li><li> Tagliare il bracciale a 4 mm dal suo bordo. </li></ol>

<ol>
	<li>Oldani, G., Lacotte, S., Morel, P., Mentha, G., Toso, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+Liver+Transplantation+in+Rats.">Orthotopic Liver Transplantation in Rats.</a> J. Vis. Exp. (65), e4143 (2012).</li><li>Hori, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comprehensive+and+innovative+techniques+for+liver+transplantation+in+rats:+A+surgical+guide.">Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide.</a> World Journal of Gastroenterology., WJG. 16, 3120 (2010).</li><li>Kashfi, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+various+techniques+of+orthotopic+liver+transplantation+in+the+rat.">A review of various techniques of orthotopic liver transplantation in the rat.</a> Transplantation Proceedings. 37 (1), 185-188 (2005).</li><li>Kamada, N., Calne, R. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Orthotopic+liver+transplantation+in+the+rat.+Technique+using+cuff+for+portal+vein+anastomosis+and+biliary+drainage.">Orthotopic liver transplantation in the rat. Technique using cuff for portal vein anastomosis and biliary drainage.</a> Transplantation. 28, 47 (1979).</li><li>Oldani, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+technique+for+rat+liver+transplantation+using+Quick+Linker+system:+a+preliminary+result.">A novel technique for rat liver transplantation using Quick Linker system: a preliminary result.</a> Journal of Surgical Research. 149, 303-309 (2008).</li></ol>

Gli autori non hanno nulla da rivelare.

La presente relazione descrive il progetto e la costruzione del sistema di quick-linker per il trapianto di fegato di ratto più facile. Trapianto di fegato di ratto è un modello popolare con 125-180 articoli correlati pubblicati annualmente dal primi anni del 1990 ( <a href="http://www.pubmed.com" target="_blank">www.pubmed.com</a> ). Esso consente un&#39;ampia gamma di esperimenti compresa l&#39;esplorazione nell&#39;ambito della conservazione e immunologiche innesto. L&#39;uso del kit &quot;quick-linker&quot; permette di anastomosi più facile e veloce con garanzia Contatto intima-a-intima. Una descrizione dettagliata e la discussione dei passaggi tecnici di un trapianto di fegato di ratto sono stati descritti in precedenza 1. Il kit rapido linker può essere costruito a basso prezzo da materiale di laboratorio comune. La sfida principale riscontrato è stata correlata la ripartizione di una maniglia durante la spazzolatura finale, e che il problema è stato risolto impostando il par più strettot dell&#39;anello maniglia a 0,5 mm (figure 1 e 2 mostrano calibro). Per quanto riguarda la qualità della lama, solo in acciaio inox deve essere considerato. Sebbene leggermente meno flessibile rispetto alla lama media in acciaio al carbonio, lame in acciaio inox non sviluppano ruggine, consentendo un uso vita delle maniglie. Di nota, il disegno delle maniglie e polsini attuale è il risultato di un processo, che ha portato il procedimento proposto, consentendo le anastomosi più efficienti. Diametri Cuff variano a seconda dei pesi di ratto (Tabella 1). Minime variazioni possono essere tollerate in termini di lunghezza, con una lunghezza usuale di circa 4 mm (figure 3 e 4). Per quanto riguarda il approssimatore, pinza del Kocher selezionata avrebbe dovuto maniglie almeno 4 cm dal fulcro. Questo permette una chiusura sicura del rack esterno addome dell&#39;animale. Complessiva,il dispositivo proposto può permettere facili anastomosi vena per il trapianto di fegato e altri tipi di innesti nei ratti.

Una tecnica di trapianto di fegato ortotopico di ratto è stato dimostrato in un recente Journal of Experiments carta Visualized 1. Vena porta e infra-epatico della vena cava anastomosi sono state eseguite utilizzando la tecnica del bracciale assistita rapido linker. Questa tecnica è stata scelta tra le altre revisioni della tecnica dell&#39;originale Kamada 2-4, in quanto consente una più facile e veloce anastomosi, si traduce in una migliore emodinamica, e può essere implementato con una curva di apprendimento più breve 1,5. La tecnica quick-linker richiede l&#39;uso di una maniglia, un approssimatore e polsini, ognuno dei quali può essere progettato in materiale laboratorio comune secondo la tecnica descritta nella presente relazione.

<table border="1">
<tbody>
 <tr>
 <td>Tool / Reagent</td>
 <td>Company</td>
 <td>Catalogue n.</td>
 <td>Comment</td>
 </tr>
 <tr>
 <td>Porta cuff</td>
 <td>altecweb or 
 smiths-medical</td>
 <td>Coil 01-96-1729 
 800/100/420</td>
 <td>Bore : variable 
 Wall : 0.30 mm 
 Length: 3.8-4.2 mm 
 <a href="http://altecweb.com" target="_blank">altecweb.com</a> or 
 <a href="http://smiths-medical.com" target="_blank">smiths-medical.com</a></td>
 </tr>
 <tr>
 <td>IVC cuff</td>
 <td>altecweb or 
 smiths-medical </td>
 <td>Coil 01-96-1733 
 800/100/540</td>
 <td>Bore : variable 
 Wall : 0.30 mm 
 Length: 3.8-4.2 mm 
 <a href="http://altecweb.com" target="_blank">altecweb.com</a> or 
 <a href="http://smiths-medical.com" target="_blank">smiths-medical.com</a></td>
 </tr>
 <tr>
 <td>Mini-drill </td>
 <td>Dremel</td>
 <td>100 series</td>
 <td>35,000 rpm 
 <a href="http://dremel.com" target="_blank">dremel.com</a></td>
 </tr>
 <tr>
 <td>Diamond wheel point</td>
 <td>Dremel</td>
 <td>7103</td>
 <td>2 mm 
 <a href="http://dremel.com" target="_blank">dremel.com</a></td>
 </tr>
 <tr>
 <td>Diamond wheel point</td>
 <td>Dremel</td>
 <td>7105</td>
 <td>4.4 mm 
 <a href="http://dremel.com" target="_blank">dremel.com</a></td>
 </tr>
 <tr>
 <td>Diamond wheel point</td>
 <td>Dremel</td>
 <td>7144</td>
 <td>2.4 mm 
 <a href="http://dremel.com" target="_blank">dremel.com</a></td>
 </tr>
 <tr>
 <td>Cut-off wheels</td>
 <td>Dremel</td>
 <td>420</td>
 <td>dia: 23.8 mm 
 thickness: 0.5 mm 
 <a href="http://dremel.com" target="_blank">dremel.com</a></td>
 </tr>
 <tr>
 <td>Quick-linker handles</td>
 <td>dltchiropody (DLT)</td>
 <td>PAR 10</td>
 <td>modified scalpel blades 
 <a href="http://dltchiropody.co.uk" target="_blank">dltchriopdy</a></td>
 </tr>
 <tr>
 <td>Quick-linker approximator</td>
 <td>pharmap</td>
 <td>UD-04-275</td>
 <td>modified Kocher's forceps 
 <a href="http://pharmap.ch" target="_blank">pharmap.ch</a></td>
 </tr>
 </tbody>
</table>

manufacturing devices and instruments for easier rat liver transplantation

Trapianto di fegato ortotopico ratto è un modello popolare, che è stato dimostrato in un articolo JoVE recente con l&#39;uso del dispositivo &quot;quick-linker&quot;. Questa tecnica consente una più facile venosa polsini anatomoses dopo una curva di apprendimento ragionevole. Il dispositivo è composto da due maniglie, che sono scavate da lame di bisturi, uno approssimatore, che si ottiene modificando pinza di Kocher, e polsini progettati da tubo di polietilene sottile-foro. L&#39;intero processo può essere eseguito ad una utilizzando materiale di laboratorio comune a basso costo. Il presente rapporto fornisce un protocollo step-by-step per la progettazione dei pezzi richiesti e comprende stencil.

Cinque kit, tra cui cinque approssimatori e 11 maniglie sono stati fabbricati. Un manico rotto durante la spazzolatura finale. Questo problema è dovuto al fatto che l&#39;anello del manico era troppo stretta, ed è stato risolto impostando la parte più stretta del ring maniglia a 0,5 mm. Non si sono verificati problemi durante la concretizzazione dei approssimatori. <table border="1"><tbody><tr><td> Peso Rat (g) </td><td> Porta Maniglia (diametro interno) (mm) </td><td> Porta cuffia (diametro esterno) (mm) </td><td> IVC maniglia (diametro interno) (mm) </td><td> IVC polsino (diametro esterno) (mm) </td></tr><tr><td> 120-180 </td><td> 2.2 </td><td> 1.7 </td><td> 2.6 </td><td> 2.08 </td></tr><tr><td> 180-270 </td><td> 2.6 </td><td> 2.08 </td><td> 3,4-3,7 </td><td> 3,0-3,25 </td></tr><tr><td> &gt; 270 </td><td> 2.9<html></td><td> 2.42 </td><td> 4.2 </td><td> 3.7 </td></tr></tbody></table> Tabella 1. <img src="/files/ftp_upload/50380/50380fig1.jpg" alt="Figura 1" fo:content-width="6in" fo:src="/files/ftp_upload/50380/50380fig1highres.jpg"/> Figura 1. Porta manico stencil. Se stampate con scala 1:1 può essere usato per guidare il manico shaping. <a href="https://www.jove.com/files/ftp_upload/50380/50380fig1large.jpg" target="_blank">Clicca qui per ingrandire la figura</a> . <img src="/files/ftp_upload/50380/50380fig2.jpg" alt="Figura 2" fo:content-width="6in" fo:src="/files/ftp_upload/50380/50380fig2highres.jpg"/> Figura 2. IVC maniglia stencil. Se stampate con scala 1:1 può essere usato per guidare il manico shaping. <a href="https://www.jove.com/files/ftp_upload/50380/50380fig2large.jpg" target="_blank">Clicca qui per ingrandire la figura</a> . contenuto &quot;&gt; <img src="/files/ftp_upload/50380/50380fig3.jpg" alt="Figura 3" fo:content-width="4.6in" fo:src="/files/ftp_upload/50380/50380fig3highres.jpg" /> Figura 3. V.Porta Porta e IVC polsini vista laterale. Del trapianto dovrebbe essere fissato al solco centrale, mentre la nave del destinatario per quella laterale. Questo espediente permette una ottimale contatto intima-a-intima. <img src="/files/ftp_upload/50380/50380fig4.jpg" alt="Figura 4" fo:content-width="4.1in" fo:src="/files/ftp_upload/50380/50380fig4highres.jpg"/> Figura 4. Porta e IVC polsini vista frontale. Pinze adatte per topi dal peso di 180-270 g sono rappresentati.

Watch this Scientific Journal Video about Manufacturing Devices and Instruments for Easier Rat Liver Transplantation at JoVE.com

Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

Descriviamo il design del dispositivo &quot;quick-linker&quot; per il trapianto di fegato di ratto ortotopico più facile.

Dispositivi di produzione e strumenti per una più facile Rat Liver Transplantation

Orthotopic rat liver transplantation is a popular model, which has been shown in a recent JoVE paper with the use of the "quick-linker" device. This ...

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8.6K Views.  University of Geneva Hospitals. Descriviamo il design del dispositivo "quick-linker" per il trapianto di fegato di ratto ortotopico più facile.

Video: Manufacturing Devices and Instruments for Easier Rat Liver Transplantation

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer

In recent years, the rapid analysis of single cells has commonly been performed using flow cytometry and fluorescently-labeled antibodies. However, the issue of spectral overlap of fluorophore emissions has limited the number of simultaneous probes. In contrast, the new CyTOF mass cytometer by DVS Sciences couples a liquid single-cell introduction system to an ICP-MS.1 Rather than fluorophores, chelating polymers containing highly-enriched metal isotopes are coupled to antibodies or other specific probes.2-5 Because of the metal purity and mass resolution of the mass cytometer, there is no "spectral overlap" from neighboring isotopes, and therefore no need for compensation matrices. Additionally, due to the use of lanthanide metals, there is no biological background and therefore no equivalent of autofluorescence. With a mass window spanning atomic mass 103-203, theoretically up to 100 labels could be distinguished simultaneously. Currently, more than 35 channels are available using the chelating reagents available from DVS Sciences, allowing unprecedented dissection of the immunological profile of samples.6-7
Disadvantages to mass cytometry include the strict requirement for a separate metal isotope per probe (no equivalent of forward or side scatter), and the fact that it is a destructive technique (no possibility of sorting recovery). The current configuration of the mass cytometer also has a cell transmission rate of only ~25%, thus requiring a higher input number of cells.
Optimal daily performance of the mass cytometer requires several steps. The basic goal of the optimization is to maximize the measured signal intensity of the desired metal isotopes (M) while minimizing the formation of oxides (M+16) that will decrease the M signal intensity and interfere with any desired signal at M+16. The first step is to warm up the machine so a hot, stable ICP plasma has been established. Second, the settings for current and make-up gas flow rate must be optimized on a daily basis. During sample collection, the maximum cell event rate is limited by detector efficiency and processing speed to 1000 cells/sec. However, depending on the sample quality, a slower cell event rate (300-500 cells/sec) is usually desirable to allow better resolution between cells events and thus maximize intact singlets over doublets and debris. Finally, adequate cleaning of the machine at the end of the day helps minimize background signal due to free metal.

The steps necessary for daily tuning and optimization of the performance of a CyTOF mass cytometer are described. Comments on optimal sample preparation and flow rate are discussed

Massa Citometria: Protocollo per l&#39;ottimizzazione giornaliera e campioni di cellule in esecuzione su un citometro Messa CyTOF

In recent years, the rapid analysis of single cells has commonly been performed using flow cytometry and fluorescently-labeled antibodies. However, the ...

Ricerca

Bioingegneria

Paper-based Devices for Isolation and Characterization of Extracellular Vesicles

Extracellular vesicles (EVs), membranous particles released from various types of cells, hold a great potential for clinical applications. They contain nucleic acid and protein cargo and are increasingly recognized as a means of intercellular communication utilized by both eukaryote and prokaryote cells. However, due to their small size, current protocols for isolation of EVs are often time consuming, cumbersome, and require large sample volumes and expensive equipment, such as an ultracentrifuge. To address these limitations, we developed a paper-based immunoaffinity platform for separating subgroups of EVs that is easy, efficient, and requires sample volumes as low as 10 &#956;l. Biological samples can be pipetted directly onto paper test zones that have been chemically modified with capture molecules that have high affinity to specific EV surface markers. We validate the assay by using scanning electron microscopy (SEM), paper-based enzyme-linked immunosorbent assays (P-ELISA), and transcriptome analysis. These paper-based devices will enable the study of EVs in the clinic and the research setting to help advance our understanding of EV functions in health and disease.

This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 &#956;l serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.

Dispositivi a base di carta per Isolamento e caratterizzazione di extracellulare vescicole

Extracellular vesicles (EVs), membranous particles released from various types of cells, hold a great potential for clinical applications. They contain ...

Procedure for Decellularization of Rat Livers in an Oscillating-pressure Perfusion Device

Decellularization and recellularization of parenchymal organs may enable the generation of functional organs in vitro, and several protocols for rodent liver decellularization have already been published. We aimed to improve the decellularization process by construction of a proprietary perfusion device enabling selective perfusion via the portal vein and/or the hepatic artery. Furthermore, we sought to perform perfusion under oscillating surrounding pressure conditions to improve the homogeneity of decellularization. The homogeneity of perfusion decellularization has been an underestimated factor to date. During decellularization, areas within the organ that are poorly perfused may still contain cells, whereas the extracellular matrix (ECM) in well-perfused areas may already be affected by alkaline detergents. Oscillating pressure changes can mimic the intraabdominal pressure changes that occur during respiration to optimize microperfusion inside the liver. In the study presented here, decellularized rat liver matrices were analyzed by histological staining, DNA content analysis and corrosion casting. Perfusion via the hepatic artery showed more homogenous results than portal venous perfusion did. The application of oscillating pressure conditions improved the effectiveness of perfusion decellularization. Livers perfused via the hepatic artery and under oscillating pressure conditions showed the best results. The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.

The presented techniques for liver harvesting, cannulation and perfusion using our proprietary device enable sophisticated perfusion set-ups to improve decellularization and recellularization experiments in rat livers.

Procedura per Decellularization di Rat Livers in un dispositivo di perfusione oscillante pressione

Decellularization and recellularization of parenchymal organs may enable the generation of functional organs in vitro, and several protocols for rodent ...

Fabricating Cotton Analytical Devices

A robust, low-cost analytical device should be user-friendly, rapid, and affordable. Such devices should also be able to operate with scarce samples and provide information for follow-up treatment. Here, we demonstrate the development of a cotton-based urinalysis (i.e., nitrite, total protein, and urobilinogen assays) analytical device that employs a lateral flow-based format, and is inexpensive, easily fabricated, rapid, and can be used to conduct multiple tests without cross-contamination worries. Cotton is composed of cellulose fibers with natural absorptive properties that can be leveraged for flow-based analysis. The simple but elegant fabrication process of our cotton-based analytical device is described in this study. The arrangement of the cotton structure and test pad takes advantage of the hydrophobicity and absorptive strength of each material. Because of these physical characteristics, colorimetric results can persistently adhere to the test pad. This device enables physicians to receive clinical information in a timely manner and shows great potential as a tool for early intervention.

To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.

Fabbricazione di dispositivi analitici cotone

A robust, low-cost analytical device should be user-friendly, rapid, and affordable. Such devices should also be able to operate with scarce samples and ...

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices cannot be worn under everyday conditions. Therefore, textiles are commonly considered the best substrate to accommodate electronic devices in wearable use. In this paper, we describe how to selectively pattern organic electroactive materials on textiles from a solution in an easy and scalable manner. This versatile deposition technique enables the fabrication of wearable organic electronic devices on clothes.

In this paper, we present a protocol to selectively deposit organic materials on textiles, which allows for the direct integration of organic electronic devices with wearables. The fabricated devices can be fully integrated in textiles, respecting their mechanical appearance and enabling sensing capabilities.

Un metodo semplice e scalabile di fabbricazione per dispositivi elettronici organici sui tessili

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices ...

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

This paper elaborates the sample preparation protocols required to obtain optimal domain patterns using the Bitter method, focusing on the extra steps compared to standard metallographic sample preparation procedures. The paper proposes a novel bespoke rig for dynamic domain imaging with in situ BH (magnetic hysteresis) measurements and elaborates the protocols for the sensor preparation and the use of the rig to ensure accurate BH measurement. The protocols for static and ordinary dynamic domain imaging (without in situ BH measurements) are also presented. The reported method takes advantage of the convenience and high sensitivity of the traditional Bitter method and enables in situ BH measurement without interrupting or interfering with the domain wall movement processes. This facilitates establishing a direct and quantitative link between the domain wall movement processes&#8211;microstructural feature interactions in ferritic steels with their BH loops. This method is anticipated to become a useful tool for the fundamental study of microstructure&#8211;magnetic property relationships in steels and to help interpret the electromagnetic sensor signals for non-destructive evaluation of steel microstructures.

Optimized Setup and Protocol for Magnetic Domain Imaging with In Situ Hysteresis Measurement

This paper elaborates the sample and sensor preparation procedures and the protocols for using the test rig particularly for dynamic domain imaging with in situ BH measurements in order to achieve optimal domain pattern quality and accurate BH measurements.

Installazione ottimizzata e protocollo per l'Imaging di dominio magnetico con In Situ isteresi misura

This paper elaborates the sample preparation protocols required to obtain optimal domain patterns using the Bitter method, focusing on the extra steps ...

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver engineering, including in vivo organ decellularization followed by repopulation, has emerged as a promising approach over ex vivo liver engineering. However, postoperative survival was not achieved. The aim of this study is to develop a novel surgical technique of in vivo selective liver lobe perfusion in rats as a prerequisite for in vivo liver engineering. We generate a circuit bypass only through the left lateral lobe. Then, the left lateral lobe is perfused with heparinized saline. The experiment is performed with 4 groups (n = 3 rats per group) based on different perfusion times of 20 min, 2 h, 3 h, and 4 h. Survival, as well as the macroscopically visible change of color and the histologically determined absence of blood cells in the portal triad and the sinusoids, is taken as an indicator for a successful model establishment. After selective perfusion of the left lateral lobe, we observe that the left lateral lobe, indeed, turned from red to faint yellow. In a histological assessment, no blood cells are visible in the branch of the portal vein, the central vein, and the sinusoids. The left lateral lobe turns red after reopening the blocked vessels. 12/12 rats survived the procedure for more than one week. We are the first to report a surgical model for in vivo single liver lobe perfusion with a long survival period of more than one week. In contrast to the previously published report, the most important advantage of the technique presented here is that perfusion of 70% of the liver is maintained throughout the whole procedure. The establishment of this technique provides a foundation for in vivo partial liver engineering in rats, including decellularization and recellularization.

A Novel Surgical Technique As a Foundation for In Vivo Partial Liver Engineering in Rat

We establish a novel surgical technique for an in vivo single liver lobe perfusion model in rat as a prerequisite for further studying in vivo partial liver engineering in the future.

Una nuova tecnica chirurgica come una Fondazione per In Vivo parziale ingegneria del fegato nel ratto

Organ engineering is a novel strategy to generate liver organ substitutes that can potentially be used in transplantation. Recently, in vivo liver ...

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Single-molecule imaging has greatly advanced our understanding of molecular mechanisms in biological studies. However, it has been challenging to obtain large field-of-view, high-contrast images in thick cells and tissues. Here, we introduce highly inclined swept tile (HIST) microscopy that overcomes this problem. A pair of cylindrical lenses was implemented to generate an elongated excitation beam that was scanned over a large imaging area via a fast galvo mirror. A 4f configuration was used to position optical components. A scientific complementary metal-oxide semiconductor camera detected the fluorescence signal and blocked the out-of-focus background with a dynamic confocal slit synchronized with the beam sweeping. We present a step-by-step instruction on building the HIST microscope with all basic components.

A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.

Una guida per costruire un altamente propenso spazzato Tile microscopio per l'esteso campo di vista singola molecola Imaging

Single-molecule imaging has greatly advanced our understanding of molecular mechanisms in biological studies. However, it has been challenging to obtain ...

Rapid Fabrication of Custom Microfluidic Devices for Research and Educational Applications

Microfluidic devices allow for the manipulation of fluids, particles, cells, micro-sized organs or organisms in channels ranging from the nano to submillimeter scales. A rapid increase in the use of this technology in the biological sciences has prompted a need for methods that are accessible to a wide range of research groups. Current fabrication standards, such as PDMS bonding, require expensive and time consuming lithographic and bonding techniques. A viable alternative is the use of equipment and materials that are easily affordable, require minimal expertise and allow for the rapid iteration of designs. In this work we describe a protocol for designing and producing PET-laminates (PETLs), microfluidic devices that are inexpensive, easy to fabricate, and consume significantly less time to generate than other approaches to microfluidics technology. They consist of thermally bonded film sheets, in which channels and other features are defined using a craft cutter. PETLs solve field-specific technical challenges while dramatically reducing obstacles to adoption. This approach facilitates the accessibility of microfluidics devices in both research and educational settings, providing a reliable platform for new methods of inquiry.

Here we present a protocol to design and fabricate custom microfluidic devices with minimal financial and time investment. The aim is to facilitate the adoption of microfluidic technologies in biomedical research laboratories and educational settings.

Rapida fabbricazione di dispositivi microfluidici personalizzati per applicazioni di ricerca e didattiche

Microfluidic devices allow for the manipulation of fluids, particles, cells, micro-sized organs or organisms in channels ranging from the nano to ...

Electric and Magnetic Field Devices for Stimulation of Biological Tissues

Electric fields (EFs) and magnetic fields (MFs) have been widely used by tissue engineering to improve cell dynamics such as proliferation, migration, differentiation, morphology, and molecular synthesis. However, variables such stimuli strength and stimulation times need to be considered when stimulating either cells, tissues or scaffolds. Given that EFs and MFs vary according to cellular response, it remains unclear how to build devices that generate adequate biophysical stimuli to stimulate biological samples. In fact, there is a lack of evidence regarding the calculation and distribution when biophysical stimuli are applied. This protocol is focused on the design and manufacture of devices to generate EFs and MFs and implementation of a computational methodology to predict biophysical stimuli distribution inside and outside of biological samples. The EF device was composed of two parallel stainless-steel electrodes located at the top and bottom of biological cultures. Electrodes were connected to an oscillator to generate voltages (50, 100, 150 and 200 Vp-p) at 60 kHz. The MF device was composed of a coil, which was energized with a transformer to generate a current (1 A) and voltage (6 V) at 60 Hz. A polymethyl methacrylate support was built to locate the biological cultures in the middle of the coil. The computational simulation elucidated the homogeneous distribution of EFs and MFs inside and outside of biological tissues. This computational model is a promising tool that can modify parameters such as voltages, frequencies, tissue morphologies, well plate types, electrodes and coil size to estimate the EFs and MFs to achieve a cellular response.

This protocol describes the step-by-step process to build both electrical and magnetic stimulators used to stimulate biological tissues. The protocol includes a guideline to simulate computationally electric and magnetic fields and manufacture of stimulator devices.

Dispositivi elettrici e a campo magnetico per la stimolazione dei tessuti biologici

Electric fields (EFs) and magnetic fields (MFs) have been widely used by tissue engineering to improve cell dynamics such as proliferation, migration, ...