Determination of Tissue-culture Infectious Dose (TCID) of the Virus
5:44
Microneutralization (MN) Assay Using MDCK-SIAT1 Cells
9:44
Results: Microneutralization Assay Results of Human Sera tested Against A/HongKong/4801/2014 A(H3N2) Virus Using MDCK-SIAT1 Cells
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Conclusion
Trascrizione
The overall goal of this microneutralization assay is to measure neutralizing antibody responses to contemporary H3N2 Influenza viruses in human sera using MDCK-SIAT1 cells. This method can be used to answer key questions to evaluate influenza imm
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Influenza neutralizing antibodies correlate with protection of influenza infections. Microneutralization assays measure neutralizing antibodies in human sera and are often used for influenza human serology. We describe a microneutralization assay using MDCK-SIAT1 cells to measure neutralizing antibody titers to contemporary 3C.2a and 3C.3a A(H3N2) viruses following influenza vaccination or infection.