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Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

12.7K Views

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08:23 min

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September 25th, 2018

DOI :

10.3791/58051-v

September 25th, 2018

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12,660 Views

Charles Decraene^1,2, Amanda Bortolini Silveira¹, Marc Michel¹, François-Clément Bidard^1,3,4, Jean-Yves Pierga^1,3,5, Marc-Henri Stern^1,6, Charlotte Proudhon¹

¹Circulating Tumor Biomarkers Laboratory, SiRIC, Translational Research Department, Institut Curie, PSL Research University, ²CNRS UMR144, Institut Curie, PSL Research University, ³Department of Medical Oncology, Institut Curie, PSL Research University, ⁴University Versailles Saint-Quentin-en-Yvelines, ⁵University Paris Descartes, ⁶Inserm U830, Institut Curie, PSL Research University

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Keywords Single Droplet Digital PCR

Circulating Tumor DNA

Mutation Detection

Hotspot Regions

Cancer Diagnosis

Absolute Quantification

Molecular Analysis

QPCR Alternative

Plasma Extraction

Silica Membrane

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