Polyacrylamide Gel Electrophoresis of Isolated and Purified Glycosaminoglycans
2:48
Silver Staining Protocol
3:55
Results: Sensitivity and Specificity of Method of Detection of GAGs
5:27
Conclusion
Trascrizione
This protocol describes a simple technique that can be used to detect the presence and measure the length of glycosaminoglycans, which are molecules of increasingly recognized significance to many biological processes. This technique is readily ad
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This report describes techniques to isolate and purify sulfated glycosaminoglycans (GAGs) from biological samples and a polyacrylamide gel electrophoresis approach to approximate their size. GAGs contribute to tissue structure and influence signaling processes via electrostatic interaction with proteins. GAG polymer length contributes to their binding affinity for cognate ligands.