Real-Time Measurements of Calcium and Contractility Parameters in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

1.7K Views

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06:42 min

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May 26th, 2023

DOI :

10.3791/65326-v

May 26th, 2023

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Rafeeh Dinani¹^,², Emmy Manders¹^,²^,³, Michiel Helmes¹^,²^,³, Lili Wang⁴, Bjorn C. Knollmann⁴, Diederik W. D. Kuster¹^,², Jolanda van der Velden¹^,²

¹Division of Physiology, Amsterdam UMC, Vrije University, ²Heart Failure & Arrhythmias, Amsterdam Cardiovascular Sciences, ³CytoCypher BV, ⁴Division of Clinical Pharmacology, Vanderbilt School of Medicine

Transcript

Summary

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Real time Measurements

Calcium Handling

Contractility Parameters

Human Induced Pluripotent Stem Cells

Cardiomyocytes

Calcium sensitive Fluorophore

Pixel Correlation

Intracellular Calcium Transients

Drug Screening

Functional Parameters

Contraction Kinetics

Preclinical Research

Hyperswitch

Ratiometric Measurements

2D Assessment

Chapters in this video

0:00

Introduction

1:13

Contractility Measurements in hiPSC‐CMs

3:39

Calcium Transient Measurements in hiPSC‐CMs

5:04

Contractility and Calcium Transient Measurements Data Analysis

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