continuous dlp printing parameter settings

連続DLPプリンティングのためのバイオインク特性の定量的評価

Tissue engineering1 is important in the field of organ repair. Due to the lack of organ donation, some diseases, such as liver failure and kidney failure, cannot be cured well, and many patients do not receive timely treatment2. Organoids with the required function of the organs may solve the problem caused by the lack of organ donation. The construction of organoids depends on the progress and development of bioprinting technology3.
Compared with extrusion-type bioprinting4 and inkjet-type bioprinting5, the printing speed and printing accuracy of the digital light processing (DLP) bioprinting method are higher6,7. The printing module of the extrusion-type method is line-by-line, while the printing module of the inkjet-type method is dot-by-dot, which is less efficient than the layer-by-layer printing module of DLP bioprinting. The modulated ultraviolet (UV) light exposure to a whole layer of material to cure a layer in DLP bioprinting and the feature size of the image determines the accuracy of DLP printing. This makes DLP technology very efficient8,9,10. Due to overcuring of the UV light, the precise relationship between the curing time and the printing size is important for high-accuracy DLP bioprinting. Furthermore, continuous DLP printing is a modification of DLP printing method that can greatly improve the printing efficiency11,12,13. For continuous DLP printing, precise printing conditions are the most important factors.
The relationship between the curing time and the printing size is called the Jacobs working curve, which is widely used in DLP printing14,15,16. The traditional method to obtain the relationship is to expose the material for a certain time and measure the curing thickness to obtain a data point about the exposure time and curing thickness. Repeating this operation at least five times and fitting the data points obtains the Jacobs working curve. However, this method has obvious disadvantages; it needs to consume a lot of material to achieve the curing, the results are highly dependent on the printing conditions, the bioinks used in DLP bioprinting are expensive and rare, and the formability of the bioinks is usually not good, which can lead to inaccurate measurements of curing thickness.
This article provides a new method to obtain the curing relationship according to the physical properties of the bioink. Using this theory can optimize continuous DLP printing. This method can be used to obtain the curing relationship more quickly and accurately; the continuous DLP curing can therefore be better determined.

著者スポットライト:デジタル光処理ベースの連続印刷のための液体感光性バイオインク特性の定量的特性評価  

連続デジタル光処理ベースの印刷のための液体感光性バイオインク特性の定量的特性評価

My research mainly involves DLP 3D bio printing and tissue engineering. We try to optimize and improve the DLP printing process and formula theory, so that the printed tissue engineering scaffold can better meet the actual clinic application needs. The printing conditions of the existing light curable materials rely on manual experiments and are obtained by feeding the data of multiple performing experiments.Such experiments waste material and reduced printing efficiency. I constructed a theoretical working curve and determined the DLP printing pyramids only based on the physical properties of the material. Dynamics both best concept is proposed to be applied in DLP printing to realize DLP format of complex dimensional structures of extremely soft materials.This method can improve the efficiency of determining the printing conditions of photo curable materials. This technology can provide technical support for the research and development of photo curable materials and the accurate printing of these materials.

Watch this Scientific Journal Video about Continuous DLP Printing Parameter Settings at JoVE.com

Continuous DLP Printing Parameter Settings  

連続 DLP 印刷パラメーターの設定

デジタル光処理またはDLP印刷を実現するには、DLPソフトウェアを使用することから始めます。パラメータ設定で、最初の単一レイヤーの露光時間をしきい値時間に設定します。この式に従って10マイクロメートルの厚さの材料を硬化させる露光時間を計算し、しきい値時間を差し引いて、単層を硬化させるための実際の露光時間を求めます。ソフトウェアのパラメータ設定で、隣接するレイヤー間の時間間隔をゼロ秒に設定します。印刷ソフトウェアのスタートボタンをクリックして、プリンタを起動します。印刷プロセスが終了したら、停止ボタンをクリックして印刷を終了します。このプロトコルは、連続DLP印刷法がさまざまな材料の理論上の作業曲線を正確に計算できることを示しています。3つの異なる材料の理論的予測と実際の印刷結果との間には、高い一致が観察されました。従来のDLP印刷方式と比較して、印刷層の厚さが薄くなり、印刷効率が向上しました。さらに、従来のDLP印刷と比較して硬化効率が10倍に向上し、連続DLP印刷方式が高効率と高忠実度を同時に達成できることを示しています。

continuous-dlp-printing-parameter-settings

Research

JoVE Journal

Bioengineering

Continuous DLP Printing Parameter Settings

127 ビュー.  Zhejiang University. デジタル光処理またはDLP印刷を実現するには、DLPソフトウェアを使用することから始めます。パラメータ設定で、最初の単一レイヤーの露光時間をしきい値時間に設定します。この式に従って10マイクロメートルの厚さの材料を硬化させる露光時間を計算し、しきい値時間を差し引いて、単層を硬化させるための実際の露光時間を求めます。ソフトウェアのパラメ?...

ビデオ: Continuous DLP Printing Parameter Settings  

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing

Precise printing fabrication of bioinks is a prerequisite for tissue engineering; the Jacobs working curve is the tool to determine the precise printing parameters of digital light processing (DLP). However, the acquisition of working curves wastes materials and requires high formability of materials, which are not suitable for biomaterials. In addition, the reduction of cell activity due to multiple exposures and the failure of structural formation due to repeated positioning are both unavoidable problems in conventional DLP bioprinting. This work introduces a new method of obtaining the working curve and the improvement process of continuous DLP printing technology based on such a working curve. This method of obtaining the working curve is based on the absorbance and photorheological properties of the biomaterials, which do not depend on the formability of the biomaterials. The continuous DLP printing process, obtained from improving the printing process by analyzing the working curve, increases the printing efficiency more than tenfold and greatly improves the activity and functionality of cells, which is beneficial to the development of tissue engineering.

This study uses temperature and material composition to control the yield stress properties of yield stress fluids. The solid-like state of the ink can protect the printing structure, and the liquid-like state can continuously fill the printing position, realizing the digital light processing 3D printing of extremely soft bioinks.

Precise printing fabrication of bioinks is a prerequisite for tissue engineering; the Jacobs working curve is the tool to determine the precise printing ...

生物工学

﻿Parameter Acquisition for DLP Printing

Parameter Acquisition for DLP Printing  

Begin by measuring the threshold time of the bio-ink using a rheometer with a temperature control element. Utilize a 365 nanometer light source to expose the testing platform of the rheometer and set the light intensity to a certain value. In the rheometer software, go to the time settings option and set the rheometer to acquire the time moduli data for 300 data points with each data point taken every 0.3 seconds.Click the start test button on the rheometer software to begin the test. Simultaneously, click the start button of the light source. To calculate the threshold time, record the corresponding time counting from the start of exposure when the storage modulus data is equal to the loss modulus data.Then build the absorbance test equipment by using upper and lower glass slides and the ring-shaped printed structure. Clamp the ring between the glass slides so that the inner circle of the ring forms the chamber. Place the chamber on the test area of the light intensity meter and set the light source to expose the chamber area.When the test chamber is not filled with material from the absorbance test equipment, measure the incident light intensity, II, by reading the display of the light intensity meter. Next, fill the test chamber with 10 microliters of bio-ink. Expose the test chamber containing bio-ink to ultraviolet light at 365 nanometers.Obtain the light intensity, IIH, by reading the display. When the value no longer changes on the light intensity meter, read the display to obtain the light intensity when the bio-ink is cured. The value is the solid absorbance, ISH.

DLP印刷のパラメータ取得

To achieve digital light processing or DLP printing, start by using a DLP software. In the parameter settings, set the exposure time of the first single layer to the threshold time. Calculate the exposure time of curing 10 micrometer thick materials according to this equation and subtract the threshold time to obtain the real exposure time for curing a single layer.In the software's parameter settings, set the time interval between adjacent layers to zero seconds. Start the printer by clicking the start button in the printing software. When the printing process ends, finish printing by clicking the stop button.This protocol demonstrates that the continuous DLP printing method can accurately calculate the theoretical working curves of different materials. A high degree of coincidence was observed between theoretical predictions and real printing results for three distinct materials. Compared to the traditional DLP printing method, this method showed improvement in printing efficiency, with decreasing printing layer thickness.Furthermore, a tenfold increase in curing efficiency was observed compared to traditional DLP printing, showing that the continuous DLP printing method can simultaneously achieve high efficiency and high fidelity.