ルシフェラーゼ生物発光レポーターを用いた遺伝子発現のモニタリング細胞自律的な概日時計のリズム

2:01

Construction of Lentiviral Luciferase Reporters

2:28

Production of Lentiviral Particles: Seed Cells (Day 1)

3:05

Transient Transfection: CaPO₄/DNA Precipitation (Day 2)

4:23

Harvest Viral Particles (Days 3-4)

5:02

Infection of 3T3 Cells (Day 3 Onward)

6:12

Synchronization and Change to Recording Medium

7:02

Bioluminescence Recording of Reporter Cells

7:26

Data Analysis and Presentation

8:12

Results: Phase-specific Circadian Reporters

9:47

Conclusion