腎細胞内のミトコンドリアの機能と細胞生存率の評価を過剰発現するプロテインキナーゼCアイソザイム

2:27

Primary Cultures of Renal Proximal Tubular Cells

5:24

Measurement of RPTC Respiration

6:18

Analysis of Mitochondrial Membrane Potential (ΔΨm)

7:14

Isolation of Mitochondria

8:16

Assaying Enzyme Complexes of the Respiratory Chain

10:24

Measurement of Intracellular ATP Content

10:55

Analysis of Cell Viability

12:18

Results: Overexpressing PKC-ε in RPTCs

14:56

Conclusion