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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

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10:01 min

June 27th, 2013

June 27th, 2013

13,523 Views

0:05

Title

1:15

Generation of X. tropicalis Eggs

3:24

Preparation of Extract from X. tropicalis Eggs

6:45

Purification of Taxol-stabilized Microtubules from X. tropicalis Extract

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Representative X. Tropicalis Extraction Results

9:37

Conclusion

We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

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