著者らは、ハエの株式の寛大な共有のためのブルーミントンストックセンター、Liqun羅、チャールズZuker、とリリーとユウ農月に感謝し、資金調達のために、以下の希望：（JDとSW）はSOMAの-URM、学士フォード学部夏フェローシップを、レニーとアンソニー・M・マーロン、MD &#39;63夏の研究フェローシップ（CLおよびDLに）（SW）は、BD社サマー研究員を経て（TO）ジェームズ・C. &#39;75とジェーンColihan夏の研究フェローシップ（DLに） （TO）にホーリークロス、ストランスキー財団サマー研究員の大学の卒業生/親サマー研究基金。ラボですべての作業をサポートするためのホーリークロスカレッジで生物学科と学部長のオフィスに感謝します。

1.十字MARCMのハエを生成するには<ol><li> 22℃で、または標準のショウジョウバエメディアコーンミール糖蜜培地（0.13％Tegosept; 23.5グラム/ Lの酵母; 60グラム/ Lのコーンミール、60ミリリットル/ Lの糖蜜; 4ミリリットル/ Lの酸ミックス6.5グラム/ Lの寒天）上のハエを維持12時間の明/暗サイクルです。 注：標準温度よりも低い、健康MARCM-株を維持するために使用されます。これは14日に卵と成人期の間の発達の時間を延長します。 </li><li>上流活性化配列（UAS-GFP）、GAL80リプレッサータンパク質、GAL4転写因子の制御下（GFPを必要な遺伝的要素を含むMARCM対応在庫から - （8日齢約1）処女雌を使用してくださいリコンビナーゼ、およびFRT組換え部位を駆動する熱ショック）。 注：私たちのMARCMすぐ銘柄は以下の遺伝子型含まれていますelavGal4、UAS-CD8-GFP、HS-flipaseを。 FRT40A、tubGal80 / CYO。 tubGal4 / TM3Sb。 注：特定のFRT部位は、突然変異の位置に依存しますゲノムインチ例えば、FRT40Aは、NOMPC（MARCMプロトコルの詳細については10を参照のこと）などの第2染色体の左腕、上の変異をテストするために使用されます。 </li><li>目的の変異とそれに対応するFRT部位含ま男性にクロスMARCM対応の処女雌（; NOMPC3、FRT40A / CYO;例えば、W-を+ / +）。効果的なMARCM誘導に制御するために、同じFRT部位を含む男性のハエを使用して第2のクロスを設定したが、突然変異のありません。十字架で1男性：女性5のおおよその比率を使用してください。 </li></ol> 2.時限産卵<ol><li>ハエは、少なくとも一晩交配させた後、新鮮な培地で新しいバイアルに大人を移動します。ハエが再び新しいバイアルに十字架を転送する前に、約4時間、暗い環境で卵を産むようにします。産卵期間の開始時間と終了時間に注意してください。 注意：4時間のintervaの終了前に、メディア上の卵の数が多いがあるように表示されたときに短い間隔が許容されますリットル。 <ol><li>熱が後で衝撃を与えたことを摂氏22度で12時間の明/暗サイクルで十分な卵の数（約20以上）とストアでバイアルを保管してください。 注：メディア上の卵の数が少ないバイアル（約20未満）は行動試験のために飛ぶと効率の利益のためにそれは彼らがさらなる試験のために保持されないことをお勧めします大人の十分な数が得られることはほとんどありません。 注意：私たちのハエは、午前8時から午後8時までにライトで発生します。午後8時と午後8時 - 午前零時 - したがって、我々は、通常4〜時限交配を行い、夕方の時間点が高い卵密度につながる発見しました。ハエが暗いキャビネット内に室温で産卵した後、インキュベーター中で、通常の明暗サイクルを乱さないように、次の日にインキュベーターに戻します。 </li></ol></li></ol> 3.有糸分裂組換えを誘導するために熱ショックを実行します<ol><li>約85 - 産卵後100時間、場所のV1時間37℃で水浴にIALS。水位がバイアル内のメディアの高さの上に達したが、完全に溺れてから幼虫を防ぐためにバイアルを水没しないことを確認してください。 </li><li>水浴からバイアルを取り出し、室温で1時間の回復期間を可能にします。 </li><li>バック1時間、摂氏37度の水浴中にバイアルを置きます。 </li><li>羽化するまで22℃で12時間の明/暗サイクルでインキュベーターで水浴とストアからバイアルを取り外します。 </li></ol>行動試験のための準備4。 <ol><li>羽化後、氷上でハエを麻酔。 MARCMのためのすべての遺伝的要素を含み、GFP発現マーキングされたクローンは、おそらく親銘柄で使用観察可能な表現型マーカーに基づいて観察される示す遺伝子型を有するハエを選択します。虹彩切除術ハサミでハエを斬ります。 注：例えば、私たちは遺伝子マーカーなしでハエのためCYO（カーリー翼）及びSb（無精ひげ毛）同定するために、選択されまし​​たモザイク領域を作成するために必要なすべての遺伝的要素を含むことになる年度ハエは、GFPでマークされました。 注意：斬首が刺激されると飛んから大人を防止する必要があります。 </li><li>閉じられた、湿った環境でのヘッドレスハエを置き、約10可能に - 回復の20時間を。右自身さらなる試験のため乱さハエをのみを使​​用します。テストは断頭の30時間以内に飛びます。 </li></ol> 5.行動試験<ol><li>蛍光解剖顕微鏡下で断頭ハエを観察することによって、毛外部感覚器官のGFPでマークされたホモ接合クローンを同定します。剛毛名および左側または右側を記録します。任意の潜在的なバイアスを排除するために、毛刺激を行うラボメンバーは毛、野生型または変異体の遺伝子型に盲目でなければなりません。 </li><li>硬い髪や細かい鉗子でフライ本体に向かって毛と脚の再を観察マークされたGFPを偏向させることによりグルーミング反射を引き出しますsponse。彼らの足を移動しないハエに刺激し、ゼロのスコアを毛に応じて自分の足を持ち上げハエに1のスコアを与えます。 注：以前の研究6,7は、特定の毛を刺激することによって誘発された足の応答を特徴としています。これは、各剛毛に応答足とその特定の毛の刺激に反応するハエの割合が含まれています。私たちは私たちがVandervorstの分類6に基づいて対応することが期待足の動きを記録しました。 </li><li>離れて2分間隔の5件の試験を実施します。 </li></ol>

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	<li>Gr&#252;nert, U., Gnatzy, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=K++and+Ca+++in+the+receptor+lymph+of+arthropod+cuticular+mechanoreceptors.">K+ and Ca++ in the receptor lymph of arthropod cuticular mechanoreceptors.</a> J Comp Physiol A. 161, 329-333 (1987).</li><li>Kernan, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanotransduction+and+auditory+transduction+in+Drosophila.">Mechanotransduction and auditory transduction in Drosophila.</a> Pflugers Arch. 454, 703-720 (2007).</li><li>Gillespie, P. G., Walker, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+basis+of+mechanosensory+transduction.">Molecular basis of mechanosensory transduction.</a> Nature. 413, 194-202 (2001).</li><li>Corfas, G., Dudai, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adaptation+and+fatigue+of+a+mechanosensory+neuron+in+wild-type+Drosophila+and+in+memory+mutants.">Adaptation and fatigue of a mechanosensory neuron in wild-type Drosophila and in memory mutants.</a> J Neurosci. 10, 491-499 (1990).</li><li>Kernan, M., Cowan, D., Zuker, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+dissection+of+mechanosensory+transduction:+mechanoreception-defective+mutations+of+Drosophila.">Genetic dissection of mechanosensory transduction: mechanoreception-defective mutations of Drosophila.</a> Neuron. 12, 1195-1206 (1994).</li><li>Vandervorst, P., Ghysen, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+control+of+sensory+connections+in+Drosophila.">Genetic control of sensory connections in Drosophila.</a> Nature. 286, 65-67 (1980).</li><li>Corfas, G., Dudai, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Habituation+and+dishabituation+of+a+cleaning+reflex+in+normal+and+mutant+Drosophila.">Habituation and dishabituation of a cleaning reflex in normal and mutant Drosophila.</a> J Neurosci. 9, 56-62 (1989).</li><li>Chen, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Discovery-based+science+education:+functional+genomic+dissection+in+Drosophila+by+undergraduate+researchers.">Discovery-based science education: functional genomic dissection in Drosophila by undergraduate researchers.</a> PLoS Biol. 3, e59 (2005).</li><li>Lee, T., Luo, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mosaic+analysis+with+a+repressible+cell+marker+for+studies+of+gene+function+in+neuronal+morphogenesis.">Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis.</a> Neuron. 22, 451-461 (1999).</li><li>Wu, J. S., Luo, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+protocol+for+mosaic+analysis+with+a+repressible+cell+marker+(MARCM)+in+Drosophila.">A protocol for mosaic analysis with a repressible cell marker (MARCM) in Drosophila.</a> Nature Protocols. 1, 2583-2589 (2006).</li><li>Eberl, D. F., Hardy, R. W., Kernan, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetically+similar+transduction+mechanisms+for+touch+and+hearing+in+Drosophila.">Genetically similar transduction mechanisms for touch and hearing in Drosophila.</a> J Neurosci. 20, 5981-5988 (2000).</li><li>Walker, R. G., Willingham, A. T., Zuker, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Drosophila+mechanosensory+transduction+channel.">A Drosophila mechanosensory transduction channel.</a> Science. 287, 2229-2234 (2000).</li><li>Yan, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila+NOMPC+is+a+mechanotransduction+channel+subunit+for+gentle-touch+sensation.">Drosophila NOMPC is a mechanotransduction channel subunit for gentle-touch sensation.</a> Nature. 493, 221-225 (2013).</li><li>Arnadottir, J., Chalfie, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Eukaryotic+Mechanosensitive+Channels.">Eukaryotic Mechanosensitive Channels.</a> Annual Review of Biophysics. 39, 111-137 (2010).</li><li>Christensen, A. P., Corey, D. 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著者は全く競合する金融利害関係はありません。

このプロトコルは 、ショウジョウバエでmechanosensationに影響を与える変異をスクリーニングするために、大人の行動アッセイを利用しています。突然変異体のコレクションは、成人としてのスクリーニング不可能に致死P因子の変異が含まれているため、このプロトコルは最初のリーと羅によって記述複雑な遺伝的手法を利用しています、（1999）、成人の致死性を回避するための...

Humans rely on the ability to convert mechanical stimuli from their environment, such as touch, pressure, vibration, or sound waves, into sensory information that can be processed by the nervous system, in a process termed mechanotransduction. Many of the overall mechanistic features of mechanotransduction between humans and invertebrates are the same1, making Drosophila a useful model to study the molecular mechanisms of mechanotransduction. Drosophila melanogaster contain two sets of specialized sensory organs (Type I and II) that are capable of converting mechanical stimuli into action potentials. Type I mechanoreceptors have a neuron with a single dendrite or sensory process, surrounded by three support cells2. The Type I mechanoreceptors include bristle mechanoreceptors, hearing sensitive chordotonal organs (the Johnston's organ), and campaniform sensilla that convey information about wing beats3. The bristles that cover the dorsal side of the fly are the most abundant and easily accessible of the Type I organs. Similar to the extracellular environment of hair cells associated with hearing and balance in vertebrates, the support cells surrounding fly mechanosensitive neurons secrete a high potassium endolymph that creates an unusual concentration gradient for potassium1. Mechanosensitive neurons, of both the mammalian and fly systems, utilize this high extracellular potassium to depolarize the cell. In response to mechanical stimulation of the macrochaete bristles towards the body wall, the sensory neurons respond with a burst of action potentials driven by this potassium depolarization of the cell membrane4. The Drosophila sensory neurons that innervate bristles resemble the mechanosensitive cells of other organisms, including vertebrate hair cells, in both structure and function1,5. The accessibility of Drosophila external sense organs to experimental manipulation and the abundance of genetic techniques available to researchers make Drosophila an excellent model system to investigate the molecular underpinnings of mechanosensation. 
In the fly, stimulation of a single sensory neuron that innervates a bristle leads to an observable behavioral response. Stimulation of different bristles evokes specific, reproducible behavioral responses, depending on the bristle that is stimulated. Upon tactile stimulation, decapitated wild-type flies exhibit a complex grooming reflex wherein they clean the area near the stimulated bristle with a patterned set of leg movements5-7. When homozygous for known single-gene mutations that interfere with learning7 or coordination and locomotor activity5, flies respond abnormally to mechanical stimulation. This grooming reflex is therefore a useful tool to study the effects of single-gene mutations on a specific, replicable behavior.
The robust behavioral response to stimulation of a single macrochaete bristle holds the potential to assist in identifying new genes involved in mechanotransduction. This protocol being used to test a collection of mutant flies for the absence of a behavioral response to indicate that the mutation interferes with mechanosensation. In the mutant collection selected for screening, the mutations cause lethality before adulthood, and therefore would be impossible to test using traditional adult behavior screens. Originally, this collection of lethal p-elements was combined with FRT recombination sites to test cell growth defects in clones. The clones were made specifically in the eye because adult flies can survive in the lab setting without functional vision8. However, removal of all mechanosensation can cause adults to be severely uncoordinated or die before eclosion5. This protocol uses a mosaic approach to circumvent the lethality of the mutations and allow for adult stage testing. A genetic technique called Mosaic Analysis with a Repressible Cell Marker (MARCM)9 is used to generate homozygous mutant cells in a limited number of adult fly sensory organs, while the rest of the organism remains heterozygous. These MARCM flies readily survive until adulthood, yet the bristles are homozygous for the lethal genes of interest.
MARCM allows for regions of homozygous mutant cells to be generated and marked by the expression of green fluorescent protein (GFP), while the rest of the organism remains heterozygous at that particular locus and unmarked9. MARCM combines individual p-element mutations with five common genetic elements: GFP under the control of an upstream-activating sequence (UAS-GFP), Gal80 repressor protein under control of a general promoter (tub-Gal80), Gal4 transcription factor under control of a general promoter (tub-Gal4), the FRT recombinase enzyme expressed through a heat-shock controlled promoter, and a FRT recombination site10. By driving mitotic recombination through a heat-shock activated recombinase, a limited number of cells are made homozygous for the mutation and marked with GFP. GFP expression is repressed in heterozygous cells by the presence of Gal80 on the wild-type copy of the chromosome. 
A heat-shock protocol for MARCM was optimized to induce recombination in the fly bristle external sense organs, while much of the organism remains heterozygous, and thus unmarked with GFP. Mosaic flies generated using this protocol contained homozygous mutations most frequently in the post alar or dorsal central bristles on the surface of the notum. 
We have tested the utility of this combination of MARCM and the grooming behavior screen with a known mechanosensitive mutant, NOMPC. The ion channel, no mechanoreceptor potential C (NOMPC), is an essential component of the mechanotransduction pathway in Drosophila5,11-13. NOMPC belongs to the transient receptor potential (TRP) superfamily of cation channels5 and satisfies all of the criteria to qualify as a mechanosensitive channel in Drosophila14,15: 1) NOMPC is expressed in the ciliate tips of type 1 sensory neurons of Drosophila 13,16-18, 2) NOMPC null larvae do not have an electrical response to tactile stimulation13, 3) Ectopic expression of NOMPC in touch insensitive cells can induce sensitivity to mechanical stimulation13, 4) heterologous expression of NOMPC in Schneider 2 cells yields a mechanosensitive channel13, and 5) NOMPC adult mutants display defects in their response to mechanical stimulation5. Given this evidence, we predicted that NOMPC mutant clones would show an altered or inhibited grooming response in response to mechanical stimulation of bristles.
A MARCM-stock containing the NOMPC mutation was developed for use in a proof of principle experiment of the grooming assay. Mosaic flies were stimulated at macrochaete bristles containing homozygous NOMPC mutant cells. We expected an inhibition of the grooming response following stimulation of the macrochaete bristle. We found that only 2 of 14 mutant bristle flies tested gave a single response to repeated stimulation; most did not respond to stimulation of the homozygous mutant bristle. Having confirmed that this MARCM-based behavioral assay produces an abnormal grooming reflex in a known mechanosensitive mosaic mutant, this technique can be used in a screen for additional mechanosensitive mutations.

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	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Brewers Yeast (25 lb)</td>
			<td style="width:163px;">MP Biomedicals&#160;</td>
			<td style="width:163px;">ICN90331225&#160;</td>
			<td style="width:163px;">Fly Food</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Corn (25lb)</td>
			<td style="width:163px;">MP Biomedicals&#160;</td>
			<td style="width:163px;">ICN90141125&#160;</td>
			<td style="width:163px;">Fly Food</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Agar (1lb)</td>
			<td style="width:163px;">MoorAgar Inc.</td>
			<td style="width:163px;">41004</td>
			<td style="width:163px;">Fly Food</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Tegosept (5kg)</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">20-259</td>
			<td style="width:163px;">Fly Food</td>
		</tr>
		<tr height="39">
			<td height="39" style="height:39px;width:163px;">Molasses (1Gallon)</td>
			<td style="width:163px;">Sugarmill Brand - Thomsen Food Service</td>
			<td style="width:163px;">0 2625</td>
			<td style="width:163px;">Fly Food</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Propionic Acid</td>
			<td style="width:163px;">Fisher</td>
			<td style="width:163px;">A258-500</td>
			<td style="width:163px;">Fly Food</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Phosphoric acid</td>
			<td style="width:163px;">Fisher</td>
			<td style="width:163px;">A260-500</td>
			<td style="width:163px;">Fly Food</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:163px;">Drosophila Vials, Narrow (PS)</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">32-109</td>
			<td style="width:163px;">Fly Cultures</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:163px;">6oz Square Bottom Bottle (PP)</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">32-130</td>
			<td style="width:163px;">Fly Cultures</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Flugs - Plastic Fly Bottles</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">49-100</td>
			<td style="width:163px;">Fly Cultures</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Rayon Balls, Large</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">51-100</td>
			<td style="width:163px;">Fly Cultures</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Droso-Filler, Narrow</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">59-168</td>
			<td style="width:163px;">Fly Food Preparation</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Droso-Filler, Bottles</td>
			<td style="width:163px;">Genesee</td>
			<td style="width:163px;">59-170</td>
			<td style="width:163px;">Fly Food Preparation</td>
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		<tr height="95">
			<td height="95" style="height:95px;width:163px;">8A-C / gear driven lab stirrer with c-clamp mount&#160; 1/15HP, 700RPM variable speed, 115V, 50/60Hz</td>
			<td style="width:163px;">Cleveland Mixer</td>
			<td style="width:163px;">8A-C</td>
			<td style="width:163px;">Fly Food Preparation</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Water jacketed Kettle</td>
			<td style="width:163px;"></td>
			<td style="width:163px;"></td>
			<td style="width:163px;">Fly Food Preparation</td>
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		<tr height="63">
			<td height="63" style="height:63px;width:163px;">Diurnal Growth Chamber</td>
			<td style="width:163px;">Forma Scientific</td>
			<td style="width:163px;"></td>
			<td style="width:163px;">Temperature and light/dark cycle controlled</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Water bath</td>
			<td style="width:163px;">VWR</td>
			<td style="width:163px;"></td>
			<td style="width:163px;">For heat shock</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">MicroScissors&#160;</td>
			<td style="width:163px;">Fine Science Tools</td>
			<td style="width:163px;">&#160;15000-08</td>
			<td style="width:163px;">For removing heads</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:163px;">Fluroscence Dissecting Microscope</td>
			<td style="width:163px;">Zeiss</td>
			<td style="width:163px;">SteREO Discovery V8&#160;</td>
			<td style="width:163px;">With GFP cube (KSC295-814D) band pass filter</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:163px;">Fluroscence Light Source</td>
			<td style="width:163px;">Zeiss</td>
			<td style="width:163px;">X-Cite 120</td>
			<td style="width:163px;">Fiber optic light pipe makes this easy to configure&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Camera for Scope</td>
			<td style="width:163px;">Zeiss</td>
			<td style="width:163px;">AxioCam ICc1</td>
			<td style="width:163px;"></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:163px;">Image acquistion software</td>
			<td style="width:163px;">Zeiss</td>
			<td style="width:163px;"></td>
			<td style="width:163px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:163px;">Ice bucket</td>
			<td style="width:163px;"></td>
			<td style="width:163px;"></td>
			<td style="width:163px;">for cold anthesia</td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:163px;">Homemade cold anthesia tray</td>
			<td style="width:163px;"></td>
			<td style="width:163px;"></td>
			<td style="width:163px;">for cold anthesia decapitation</td>
		</tr>
		<tr height="63">
			<td height="63" style="height:63px;width:163px;">Plastic boxes</td>
			<td style="width:163px;"></td>
			<td style="width:163px;"></td>
			<td style="width:163px;">for recovery of decaptitated flies in humid environment</td>
		</tr>
	</tbody>
</table>

a behavioral assay for mechanosensation of marcm-based clones in drosophila melanogaster

そのため哺乳類の内耳の有毛細胞への構造的および機能的相同性のため、 ショウジョウバエの外部感覚器官を神経支配するニューロンはmechanosensationの研究のための優れたモデルシステムを提供します。このプロトコルは、mechanosensationを妨害する変異を同定するために使用することができショウジョウバエでシンプルなタッチの動作について説明します。 macrochaeteの触覚刺激は、ハエの胸郭に毛のいずれかの第1または第3の脚からグルーミング反射を誘発します。 （例えばNOMPCなど）メカノと、または反射弓の他の側面に干渉する突然変異は、グルーミング応答を阻害することができます。大人の行動の伝統的な画面は開発中の重要な役割を持っている変異体を逃したであろう。代わりに、このプロトコルは、exprで生成され、マークされたホモ接合変異体の細胞の限られた領域を可能にするために、抑制性細胞マーカー（MARCM）でモザイク解析でタッチスクリーンを組み合わせました緑色蛍光タンパク質（GFP）のession。異常行動応答についてMARCMクローンを試験することにより、より伝統的な方法では見逃されたであろうmechanosensationに関与する新しい遺伝子を検索するために致死P因子突然変異のコレクションをスクリーニングすることが可能です。

このプロトコルの成功は、主にテスト可能なハエの全収率に依存して、熱ショックの効果は、有糸分裂組換え、2分間隔で刺激されたハエにおける強固な行動反応を得る能力を誘導します。胚の推定12.5％が必要MARCM要素およびG​​FP発現の可能性を有する孵化する成人の推定16.67％を含有することを考えると、それは時限中の卵の高い数を得ることが重要である産みま?...

Watch this Scientific Journal Video about A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster at JoVE.com

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

でMARCMベースのクローンのMechanosensationための行動アッセイ<em&gt;キイロショウジョウバエ</em

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

Because of the structural and functional homology to the hair cells of the mammalian inner ear, the neurons that innervate the Drosophila external sense ...

a-behavioral-assay-for-mechanosensation-marcm-based-clones-drosophila

Research

JoVE Journal

Neuroscience

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

10.2K ビュー.  College of the Holy Cross. In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

ビデオ: A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

A General Method for Evaluating Incubation of Sucrose Craving in Rats

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and relapse to food taking 1. Food craving conceptualized in this way is akin to drug craving in response to drug-paired cues. A rich literature has been developed around understanding the behavioral and neurobiological determinants of drug craving; we and others have been focusing recently on translating techniques from basic addiction research to better understand addiction-like behaviors related to food 2-4.
As done in previous studies of drug craving, we examine sucrose craving behavior by utilizing a rat model of relapse. In this model, rats self-administer either drug or food in sessions over several days. In a session, lever responding delivers the reward along with a tone+light stimulus. Craving behavior is then operationally defined as responding in a subsequent session where the reward is not available. Rats will reliably respond for the tone+light stimulus, likely due to its acquired conditioned reinforcing properties 5. This behavior is sometimes referred to as sucrose seeking or cue reactivity. In the present discussion we will use the term "sucrose craving" to subsume both of these constructs.
In the past decade, we have focused on how the length of time following reward self-administration influences reward craving. Interestingly, rats increase responding for the reward-paired cue over the course of several weeks of a period of forced-abstinence. This "incubation of craving" is observed in rats that have self-administered either food or drugs of abuse 4,6. This time-dependent increase in craving we have identified in the animal model may have great potential relevance to human drug and food addiction behaviors.
Here we present a protocol for assessing incubation of sucrose craving in rats. Variants of the procedure will be indicated where craving is assessed as responding for a discrete sucrose-paired cue following extinction of lever pressing within the sucrose self-administration context (Extinction without cues) or as responding for sucrose-paired cues in a general extinction context (Extinction with cues).

Responding for food or drug-paired cues increases over the course of a period of abstinence and this may relate to an increased susceptibility to relapse behaviors. Here we detail a procedure for evaluating this "incubation of craving" in rats that have self-administered sucrose.

ラットのショ糖の渇望のインキュベーションを評価するための一般的な方法

For someone on a food-restricted diet, food craving in response to food-paired cues may serve as a key behavioral transition point between abstinence and ...

神経科学

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Screening compounds for in vivo activity can be used as a first step to identify candidates that may be developed into pharmacological agents1,2. We developed a novel nanoinjection/electrophysiology assay that allows the detection of bioactive modulatory effects of compounds on the function of a neuronal circuit that mediates the escape response in Drosophila melanogaster3,4. Our in vivo assay, which uses the Drosophila Giant Fiber System (GFS, Figure 1) allows screening of different types of compounds, such as small molecules or peptides, and requires only minimal quantities to elicit an effect. In addition, the Drosophila GFS offers a large variety of potential molecular targets on neurons or muscles. The Giant Fibers (GFs) synapse electrically (Gap Junctions) as well as chemically (cholinergic) onto a Peripheral Synapsing Interneuron (PSI) and the Tergo Trochanteral Muscle neuron (TTMn)5. The PSI to DLMn (Dorsal Longitudinal Muscle neuron) connection is dependent on D&alpha;7 nicotinic acetylcholine receptors (nAChRs)6. Finally, the neuromuscular junctions (NMJ) of the TTMn and the DLMn with the jump (TTM) and flight muscles (DLM) are glutamatergic7-12. Here, we demonstrate how to inject nanoliter quantities of a compound, while obtaining electrophysiological intracellular recordings from the Giant Fiber System13 and how to monitor the effects of the compound on the function of this circuit. We show specificity of the assay with methyllycaconitine citrate (MLA), a nAChR antagonist, which disrupts the PSI to DLMn connection but not the GF to TTMn connection or the function of the NMJ at the jump or flight muscles.
Before beginning this video it is critical that you carefully watch and become familiar with the JoVE video titled "Electrophysiological Recordings from the Giant Fiber Pathway of D. melanogaster " from Augustin et al7, as the video presented here is intended as an expansion to this existing technique. Here we use the electrophysiological recordings method and focus in detail only on the addition of the paired nanoinjections and monitoring technique.

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.

用いて、化合物の生理活性をスクリーニングするためのペアNanoinjectionと電気生理学的アッセイキイロショウジョウバエジャイアントファイバシステム

Screening compounds for in vivo activity can be used as a first step to identify candidates that may be developed into pharmacological agents1,2. We ...

A Lightweight, Headphones-based System for Manipulating Auditory Feedback in Songbirds

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and sensorimotor plasticity1. Consistent sensory perturbations result in compensatory changes in motor output, reflecting changes in feedforward motor control that reduce the experienced feedback error. By quantifying how different sensory feedback errors affect human behavior, prior studies have explored how visual signals are used to recalibrate arm movements2,3 and auditory feedback is used to modify speech production4-7. The strength of this approach rests on the ability to mimic naturalistic errors in behavior, allowing the experimenter to observe how experienced errors in production are used to recalibrate motor output.
Songbirds provide an excellent animal model for investigating the neural basis of sensorimotor control and plasticity8,9. The songbird brain provides a well-defined circuit in which the areas necessary for song learning are spatially separated from those required for song production, and neural recording and lesion studies have made significant advances in understanding how different brain areas contribute to vocal behavior9-12. However, the lack of a naturalistic error-correction paradigm - in which a known acoustic parameter is perturbed by the experimenter and then corrected by the songbird - has made it difficult to understand the computations underlying vocal learning or how different elements of the neural circuit contribute to the correction of vocal errors13.
The technique described here gives the experimenter precise control over auditory feedback errors in singing birds, allowing the introduction of arbitrary sensory errors that can be used to drive vocal learning. Online sound-processing equipment is used to introduce a known perturbation to the acoustics of song, and a miniaturized headphones apparatus is used to replace a songbird's natural auditory feedback with the perturbed signal in real time. We have used this paradigm to perturb the fundamental frequency (pitch) of auditory feedback in adult songbirds, providing the first demonstration that adult birds maintain vocal performance using error correction14. The present protocol can be used to implement a wide range of sensory feedback perturbations (including but not limited to pitch shifts) to investigate the computational and neurophysiological basis of vocal learning.

We describe the design and assembly of miniaturized headphones suitable for replacing a songbird&#x2019;s natural auditory feedback with a manipulated acoustic signal. Online sound processing hardware is used to manipulate song output, introduce real-time errors in auditory feedback via the headphones, and drive vocal motor learning.

鳴禽類には聴覚フィードバックを操作するための軽量、ヘッドフォンベースのシステム

Experimental manipulations of sensory feedback during complex behavior have provided valuable insights into the computations underlying motor control and ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

A growing number of genetically encoded tools are becoming available that allow non-invasive manipulation of the neural activity of specific neurons in Drosophila melanogaster1. Chief among these are optogenetic tools, which enable the activation or silencing of specific neurons in the intact and freely moving animal using bright light. Channelrhodopsin (ChR2) is a light-activated cation channel that, when activated by blue light, causes depolarization of neurons that express it. ChR2 has been effective for identifying neurons critical for specific behaviors, such as CO2 avoidance, proboscis extension and giant-fiber mediated startle response2-4. However, as the intense light sources used to stimulate ChR2 also stimulate photoreceptors, these optogenetic techniques have not previously been used in the visual system. Here, we combine an optogenetic approach with a mutation that impairs phototransduction to demonstrate that activation of a cluster of loom-sensitive neurons in the fly's optic lobe, Foma-1 neurons, can drive an escape behavior used to avoid collision. We used a null allele of a critical component of the phototransduction cascade, phospholipase C-&beta;, encoded by the norpA gene, to render the flies blind and also use the Gal4-UAS transcriptional activator system to drive expression of ChR2 in the Foma-1 neurons. Individual flies are placed on a small platform surrounded by blue LEDs. When the LEDs are illuminated, the flies quickly take-off into flight, in a manner similar to visually driven loom-escape behavior. We believe that this technique can be easily adapted to examine other behaviors in freely moving flies.

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Genetically encoded optogenetic tools enable noninvasive manipulation of specific neurons in the Drosophila brain. Such tools can identify neurons whose activation is sufficient to elicit or suppress particular behaviors. Here we present a method for activating Channelrhodopsin2 that is expressed in targeted neurons in freely walking flies.

で逃避行動のOptogenetic刺激<em&gt;キイロショウジョウバエ</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

A Single-fly Assay for Foraging Behavior in Drosophila

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster. In a light-tight box illuminated by red light that is invisible to fruit flies, a camera linked to custom data acquisition software monitors the position of six flies simultaneously. Each fly is confined to walk in individual arenas containing a food odor at the center. The testing arenas rest on a porous floor that functions to prevent odor accumulation. Latency to locate the odor source, a metric that reflects olfactory sensitivity under different physiological states, is determined by software analysis. Here, we discuss the critical mechanics of running this behavioral paradigm and cover specific issues regarding fly loading, odor contamination, assay temperature, data quality, and statistical analysis.

A Single-fly Assay for Foraging Behavior in Drosophila

In this video article, we describe an automated assay to measure the effect of hunger or satiety on olfactory dependent food search behavior in the adult fruit fly Drosophila melanogaster.

での採餌行動のためのシングル·フライアッセイ<em&gt;ショウジョウバエ</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis

Measurement of the visual function in the tadpoles of the frog, Xenopus laevis, allows screening for blindness in live animals. The optokinetic response is a vision-based, reflexive behavior that has been observed in all vertebrates tested. Tadpole eyes are small so the tail flip response was used as alternative measure, which requires a trained technician to record the subtle response. We developed an alternative behavior assay based on the fact that tadpoles prefer to swim on the white side of a tank when placed in a tank with both black and white sides. The assay presented here is an inexpensive, simple alternative that creates a response that is easily measured. The setup consists of a tripod, webcam and nested testing tanks, readily available in most Xenopus laboratories. This article includes a movie showing the behavior of tadpoles, before and after severing the optic nerve. In order to test the function of one eye, we also include representative results of a tadpole in which each eye underwent retinal axotomy on consecutive days. Future studies could develop an automated version of this assay for testing the vision of many tadpoles at once.

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis

Xenopus laevis tadpoles prefer swimming on the white side of a black/white tank. This behavior is guided by their vision. Based on this behavior, we present a simple assay to test the visual function of tadpoles.

テスト視機能のための簡単​​な行動アッセイ<em&gt;アフリカツメガエル</em

Measurement of the visual function in the tadpoles of the frog, Xenopus laevis, allows screening for blindness in live animals. The optokinetic response ...

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

Drosophila melanogaster is an emerging model to study different aspects of social interactions. For example, flies avoid areas previously occupied by stressed conspecifics due to an odorant released during stress known as the Drosophila stress odorant (dSO). Through the use of the T-maze apparatus, one can quantify the avoidance of the dSO by responder flies in a very affordable and robust assay. Conditions necessary to obtain a strong performance are presented here. A stressful experience is necessary for the flies to emit dSO, as well as enough emitter flies to cause a robust avoidance response to the presence of dSO. Genetic background, but not their group size, strongly altered the avoidance of the dSO by the responder flies. Canton-S and Elwood display a higher performance in avoiding the dSO than Oregon and Samarkand strains. This behavioral assay will allow identification of mechanisms underlying this social behavior, and the assessment of the influence of genes and environmental conditions on both emission and avoidance of the dSO. Such an assay can be included in batteries of simple diagnostic tests used to identify social deficiencies of mutants or environmental conditions of interest.

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

Here, we present a protocol to quantify the avoidance of stressed individuals. This paradigm is powerful yet user-friendly and can be used to assess the influence of genes and environment on one kind of social interaction in Drosophila melanogaster.

社会回避での定量化のための簡単​​なアッセイ<em&gt;キイロショウジョウバエ</em

Drosophila melanogaster is an emerging model to study different aspects of social interactions. For example, flies avoid areas previously occupied by ...

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila

Mating experiments using Drosophila have contributed greatly to the understanding of sexual selection and behavior. Experiments often require simple, easy and cheap methods to distinguish between individuals in a trial. A standard technique for this is CO2 anaesthesia and then labelling or wing clipping each fly. However, this is invasive and has been shown to affect behavior. Other techniques have used coloration to identify flies. This article presents a simple and non-invasive method for labelling Drosophila that allows them to be individually identified within experiments, using food coloring. This method is used in trials where two males compete to mate with a female. Dyeing allowed quick and easy identification. There was, however, some difference in the strength of the coloration across the three species tested. Data is presented showing the dye has a lower impact on mating behavior than CO2 in Drosophila melanogaster. The impact of CO2 anaesthesia is shown to depend on the species of Drosophila, with D. pseudoobscura and D. subobscura showing no impact, whereas D. melanogaster males had reduced mating success. The dye method presented is applicable to a wide range of experimental designs.

Dyeing Insects for Behavioral Assays: the Mating Behavior of Anesthetized Drosophila

This protocol describes a simple, cost effective way to individually identify Drosophila or other insects. Demonstration data investigating mating success across three species of Drosophila show that this method is comparable or better than the use of CO2 anaesthesia.

行動アッセイの染色昆虫：麻酔の配偶行動<em&gt;ショウジョウバエ</em

Mating experiments using Drosophila have contributed greatly to the understanding of sexual selection and behavior. Experiments often require simple, easy ...

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

Alcohol use disorder (AUD) is a serious health challenge. Despite a large hereditary component to AUD, few genes have been unambiguously implicated in their etiology. The fruit fly, Drosophila melanogaster, is a powerful model for exploring molecular-genetic mechanisms underlying alcohol-related behaviors and therefore holds great promise for identifying and understanding the function of genes that influence AUD. The use of the Drosophila model for these types of studies depends on the availability of assays that reliably measure behavioral responses to ethanol. This report describes an assay suitable for assessing ethanol sensitivity and rapid tolerance in flies. Ethanol sensitivity measured in this assay is influenced by the volume and concentration of ethanol used, a variety of previously reported genetic manipulations, and also the length of time the flies are housed without food immediately prior to testing. In contrast, ethanol sensitivity measured in this assay is not affected by the vigor of fly handling, sex of the flies, and supplementation of growth medium with antibiotics or live yeast. Three different methods for quantitating ethanol sensitivity are described, all leading to essentially indistinguishable ethanol sensitivity results. The scalable nature of this assay, combined with its overall simplicity to set-up and relatively low expense, make it suitable for small and large scale genetic analysis of ethanol sensitivity and rapid tolerance in Drosophila.

An Inexpensive, Scalable Behavioral Assay for Measuring Ethanol Sedation Sensitivity and Rapid Tolerance in Drosophila

Straightforward assays for measuring ethanol sensitivity and rapid tolerance in Drosophila facilitate the use of this model organism for investigating these important ethanol-related behaviors. Here, a relatively simple, scalable assay for measuring ethanol sensitivity and rapid tolerance in flies is described.

エタノール鎮静感度との急速な寛容を測定するための安価な、スケーラブルな行動アッセイ<em&gt;ショウジョウバエ</em

Alcohol use disorder (AUD) is a serious health challenge. Despite a large hereditary component to AUD, few genes have been unambiguously implicated in ...

Conditions Affecting Social Space in Drosophila melanogaster

The social space assay described here can be used to quantify social interactions of Drosophila melanogaster &#8212; or other small insects &#8212; in a straightforward manner. As we previously demonstrated 1, in a two-dimensional chamber, we first force the flies to form a tight group, subsequently allowing them to take their preferred distance from each other. After the flies have settled, we measure the distance to the closest neighbor (or social space), processing a static picture with free online software (ImageJ). The analysis of the distance to the closest neighbor allows researchers to determine the effects of genetic and environmental factors on social interaction, while controlling for potential confounding factors. Diverse factors such as climbing ability, time of day, sex, and number of flies, can modify social spacing of flies. We thus propose a series of experimental controls to mitigate these confounding effects. This assay can be used for at least two purposes. First, researchers can determine how their favorite environmental shift (such as isolation, temperature, stress or toxins) will impact social spacing 1,2. Second, researchers can dissect the genetic and neural underpinnings of this basic form of social behavior 1,3. Specifically, we used it as a diagnostic tool to study the role of orthologous genes thought to be involved in social behavior in other organisms, such as candidate genes for autism in humans 4.

Conditions Affecting Social Space in Drosophila melanogaster

The effect of genes and environment on social space of Drosophila melanogaster can be quantified through a powerful but straightforward analytical paradigm. We show here different factors that can affect this social space, and thus need to be taken into consideration when designing experiments in this paradigm.

で社会空間に影響を与える条件<em&gt;キイロショウジョウバエ</em

The social space assay described here can be used to quantify social interactions of Drosophila melanogaster &#8212; or other small insects &#8212; in a ...