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JoVE Journal

Neuroscience

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Stereotactic Atlas-Guided Laser Capture Microdissection of Brain Regions Affected by Traumatic Injury

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09:29 min

September 11th, 2017

September 11th, 2017

8,948 Views

0:05

Title

0:59

Region Identification and Sectioning

4:01

Staining Protocol

4:50

Stereotactic Atlas Guided Laser Capture Microdissection with the LCM System

7:36

Results: LCM of Traumatic Brain Injury affected Brain Regions

8:55

Conclusion

文字起こし

We describe the use of laser capture microdissection to obtain samples of distinct cell populations from different brain regions for gene and microRNA analysis. This technique allows the study of differential effects of traumatic brain injury in specific regions of the rat brain.

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