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Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

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09:42 min

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October 12th, 2017

DOI :

10.3791/56446-v

October 12th, 2017

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9,431 Views

Evan Bardot¹^,²^,³, Nikos Tzavaras⁴, Deanna L. Benson⁵^,⁶, Nicole C. Dubois¹^,²^,³

¹Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, ²Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, ³Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, ⁴Microscopy Core, Icahn School of Medicine at Mount Sinai, ⁵Department of Neuroscience, Icahn School of Medicine at Mount Sinai, ⁶Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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要約

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Quantitative Whole mount Immunofluorescence

Cardiac Progenitor Populations

Mouse Embryos

Heart Development

Embryonic Organogenesis

Volumetric Imaging

Tissue level Dynamics

Paraformaldehyde Fixation

Immunofluorescence Staining

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