ありがとうモーザー ラボ カブリ研究所システム神経科学センター神経計算のため、ラットのノルウェー大学の科学と技術、慢性的な神経の記録のプロシージャ。この作品は、NIH グラント R21 NS098146 と人間フロンティア科学プログラム長期フェローシップ LT000211/2016-L を l. Lu によって支えられました。

<ol>
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著者が明らかに何もありません。

ここでは、我々 は 18 独立可動管四極管から成る新開発のハイパー ドライブを構築するプロセスを説明します。ドライブは、stereolithographic 印刷によって作成されたコンポーネントと組み合わせて、多くの利用可能なハードウェアの店で購入した手頃な価格の部分から構築できます。ハイパー ドライブの故障は慢性的な標準的な外科プロシージャを使用してラットの頭蓋骨の上に埋め込むこ?...

システム神経科学の分野では、科学者たちは、空間的ナビゲーション、記憶、意思決定などの認知プロセスの基礎となる神経機構を学ぶ。この種の研究では、動物の行動の中に多くの個々 のニューロンの活動を監視する重要です。過去十年にわたって小動物1,2,3細胞外神経記録の実験的ニーズを満たすための 2 つの重要な進歩がなされました。・ テトロード、ニューロンの神経活動を同時に記録するために使用 4 マイクロワイヤーのバンドルの開発を初めて1,2,4。差動信号振幅、熱の 4 つのチャネルの活動の多くの同時記録セル5から個々 のニューロン活動を分離できます。さらに、マイクロワイヤーの柔軟な性質は、熱とターゲット細胞集団間の相対変位を最小化・ テトロードのより高い安定性をことができます。管四極管は今様々 な種、齧歯動物1,2,6、霊長類7昆虫8など多くの脳研究の単一電極の代わりに広く使用されます。第二に、ハイパー ドライブの開発を運んでいた複数独立可動管四極管、複数記録場所3,からニューロンのより大きい人口からの神経活動の同時モニタリングが可能9,,1011,12。
小動物用の信頼性と手頃なマルチ ・ テトロードの記録装置の可用性は制限されています。自由行動下ラット、過去二十年9,10,14,の多くの演習で神経録音当初ブルース · マクノートン13、によって開発された、古典的なハイパー ドライブを正常に使用されています15. ただし、技術的な理由から、入手は非常に困難です今マクノートン ドライブを構築に必要な元のコンポーネントと最近改良されたデータ集録インタ フェースと互換性がありません。ハイパー ドライブの他のよく受け入れられている設計個別に手作りするのにマイクロ ドライブの矛盾した結果をもたらすことができるとかなりの時間12を消費する必要があります。行動中のラットのさまざまな脳領域から神経活動を記録するために、stereolithographic 技術を使用して新しいハイパー ドライブを開発しました。我々 は次の要件を満たすために求められている: (1) 脳内管四極管の正確な変位および複数ターゲット地域から安定した記録を提供する必要があります新しいハイパー ドライブ(2) の新しいハイパー ドライブは簡単な接続を許可するように最近開発された磁気 quickclip システムと互換性があります。(3) 新しいハイパー ドライブは容易に利用できる材料を正確に再現できます。ここでは、18 独立可動の管四極管をマクノートン設計に基づくを含む 3 D 印刷のハイパー ドライブを構築するための手法を提供します。プロトコルでは、我々 は使用した正常にレコードの単一ニューロンの活動電位、局所電界電位 postrhinal と内側嗅皮質からの数週間にわたって新しいハイパー ドライブの製造プロセスの詳細を説明する、自由に自然採餌タスク中にラットを動作しています。

<table><tbody><tr><td>Welding rod</td><td>Blue Demon</td><td>ER308L-035-01T</td><td>Stainless steel, 0.035&quot; in diameter</td></tr><tr><td>Screw</td><td>McMaster</td><td>91771A060</td><td>Stainless steel, flat head, 0-80&amp;nbsp;thread,&amp;nbsp;5/8&quot;&amp;nbsp;in length</td></tr><tr><td>Screw</td><td>McMaster</td><td>91772A051</td><td>Stainless steel, pan head, 0-80&amp;nbsp;thread,&amp;nbsp;5/32&quot;&amp;nbsp;in length</td></tr><tr><td>Screw</td><td>McMaster</td><td>92196A056</td><td>Stainless steel, socket head, 0-80&amp;nbsp;thread,&amp;nbsp;5/16&quot;&amp;nbsp;in length</td></tr><tr><td>Screw</td><td>McMaster</td><td>92196A055</td><td>Stainless steel, socket head, 0-80&amp;nbsp;thread,&amp;nbsp;1/4&quot;&amp;nbsp;in length</td></tr><tr><td>Screw</td><td>McMaster</td><td>95868A131</td><td>Nylon,&amp;nbsp; socket head, 2-56&amp;nbsp;thread,&amp;nbsp;3/16&quot;&amp;nbsp;in length, black</td></tr><tr><td>Screw nut</td><td>McMaster</td><td>90730A001</td><td>Stainless steel, narrow hex,&amp;nbsp; 0-80&amp;nbsp;thread</td></tr><tr><td>Shoulder screw</td><td>McMaster</td><td>90298A213</td><td>Stainless steel, 8-32 thread, 3/16&quot; in diameter, 1/4&quot; in length</td></tr><tr><td>Cup screw</td><td>McMaster</td><td>92313A105</td><td>Stainless steel, 4-40 thread, 3/16&quot; in length</td></tr><tr><td>Thumb screw</td><td>McMaster</td><td>94323A592</td><td>Nylon, 8-32&amp;nbsp;thread,&amp;nbsp;3/8&quot;&amp;nbsp;in length, black</td></tr><tr><td>Magnet</td><td>Apex</td><td>M3X1MMDI</td><td>Neodymium, 3 mm X 1 mm disc</td></tr><tr><td>Metal tubing</td><td>Small Parts</td><td>B00137QHNS</td><td>Stainless steel, 23 gauge, 0.0253&quot; OD, 0.013&quot; ID, 0.006&quot; wall</td></tr><tr><td>Metal tubing</td><td>New England Small Tube</td><td>Custom-made</td><td>Stainless steel, 30 gauge, 0.012/0.0125&quot; OD, 0.007/0.008&quot; ID, full hard</td></tr><tr><td>Heat-shrink tubing</td><td>McMaster</td><td>7856K72</td><td>0.09&quot; ID before shrinking, blue</td></tr><tr><td>Silicone tubing</td><td>A-M Systems</td><td>807300</td><td>0.040&quot; ID, 0.085&quot; OD</td></tr><tr><td>Polyimide tubing</td><td>A-M Systems</td><td>823400</td><td>0.0045&quot; ID, 0.0005&quot; wall</td></tr><tr><td>Ground wire</td><td>A-M Systems</td><td>791500</td><td>0.005&quot; bare, 0.008&quot; coated, half hard</td></tr><tr><td>Tetrode wire</td><td>California Fine Wire</td><td>Custom-made</td><td>0.0007&quot; in diameter, platinum-iridium (90%-10%), HML and VG coating</td></tr><tr><td>EIB</td><td>Neuralynx</td><td></td><td>EIB-72-QC-Large</td></tr><tr><td>Gold pins</td><td>Neuralynx</td><td></td><td>large EIB pins</td></tr><tr><td>Tap</td><td>Balax</td><td>01302-000</td><td>M1.2 thread size</td></tr><tr><td>Tap</td><td>McMaster</td><td>2522A811</td><td>0-80 thread size, bottoming</td></tr><tr><td>Tap</td><td>McMaster</td><td>2522A771</td><td>0-80 thread size, plug</td></tr><tr><td>Tap</td><td>McMaster</td><td>26955A94</td><td>3/8&quot;-24&amp;nbsp;thread size, bottoming</td></tr><tr><td>Tap</td><td>McMaster</td><td>2522A713</td><td>2-56&amp;nbsp;thread size</td></tr><tr><td>Tap</td><td>McMaster</td><td>2522A715</td><td>4-40&amp;nbsp;thread size</td></tr><tr><td>Tap</td><td>McMaster</td><td>2522A718</td><td>8-32&amp;nbsp;thread size</td></tr><tr><td>Die</td><td>McMaster</td><td>2576A457</td><td>3/8&quot;-24&amp;nbsp;thread size, 1&quot; OD</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>30585A82</td><td>Wire gauge&amp;nbsp;65,&amp;nbsp;0.035&quot; in diameter</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>30585A83</td><td>Wire gauge&amp;nbsp;66,&amp;nbsp;0.033&quot; in diameter</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>30585A87</td><td>Wire gauge&amp;nbsp;70,&amp;nbsp;0.028&quot; in diameter</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>30585A88</td><td>Wire gauge&amp;nbsp;71,&amp;nbsp;0.026&quot; in diameter</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>30585A91</td><td>Wire gauge&amp;nbsp;73,&amp;nbsp;0.024&quot; in diameter</td></tr><tr><td>Drill bit</td><td>McMaster</td><td>8870A23</td><td>3/16&quot;&amp;nbsp;in diameter</td></tr><tr><td>Dremel disc</td><td>Wagner</td><td>31M</td><td>Diamond coated, 22 mm in diameter, 0.17 mm in thickness</td></tr><tr><td>Steel wire</td><td>Precision Brand</td><td>21212</td><td>0.012&quot; in diameter, full hard</td></tr><tr><td>Steel wire</td><td>Precision Brand</td><td>21007</td><td>0.007&quot; in diameter, full hard</td></tr><tr><td>Steel wire</td><td>A-M Systems</td><td>792700</td><td>0.003&quot; in diameter, half hard</td></tr><tr><td>Super glue</td><td>Loctite</td><td>LT-40640</td><td># 406</td></tr><tr><td>Super glue</td><td>Loctite</td><td>LT-41550</td><td># 415</td></tr><tr><td>Dental acrylic powder&amp;nbsp;</td><td>Teets</td><td>223-3773</td><td>Coral</td></tr><tr><td>Dental acrylic liquid</td><td>Teets</td><td>223-4003</td><td></td></tr></tbody></table>

construction of an improved multi-tetrode hyperdrive for large-scale neural recording in behaving rats

数日間目がさめている動物のニューロンの大規模な人口の活動パターンの監視は、システム神経科学の分野で貴重な技術です。この手法の 1 つ主要なコンポーネントは、正確に必要な脳の領域に複数の電極の配置とその安定性の維持で構成されます。ここでは、18 個別調節可能な管四極管が含まれていますし、自由行動下ラットの神経細胞外記録生体内では、3 D 印刷可能なハイパー ドライブの建設のためのプロトコルについて述べる。管四極管、マイクロ ドライブに接続されているトラックに沿って複数の脳領域に個別に進めることがいずれかまたは小さい領域に電極の配列を配置する使用ことができます。複数管四極管のアクティブな動作の中に脳の神経細胞の集団から局所電界電位と同様、個々 のニューロンの数十から action potentials の同時試験を可能にします。また、デザインは原案作成ソフトウェア異なる実験的ニーズに合わせて簡単に変更できる単純な 3 D のため提供します。

試験の結果を取得するのに新築のハイパー ドライブを使用しました。ドライブは、ø 17 μ m (0.0007")、プラチナ イリジウム (90%-10%) ポリイミド被覆ワイヤー製管四極管が装備されていた。管の四極管の先端は、白金黒電極インピー ダンスを 1 kHz で 100 と 200 の kΩ 間を削減するソリューションでメッキされました。ハイパー ドライブの故障は、4.6 mm 550 g、ロング ・ ラ?...

Watch this Scientific Journal Video about Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats at JoVE.com

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

18 個別調節可能な管四極管と 3 D 印刷可能なハイパー ドライブの建設を提案します。ハイパー ドライブの故障は、ラットを数週間の期間にわたって自由に行動で脳の活動を記録するために設計されています。

ラットの行動で大規模な神経細胞記録向上のマルチ ・ テトロード ハイパー ドライブの構築

Monitoring the activity patterns of a large population of neurons over many days in awake animals is a valuable technique in the field of systems ...

construction-an-improved-multi-tetrode-hyperdrive-for-large-scale

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Neuroscience

11.2K ビュー.  Baylor College of Medicine. 18 個別調節可能な管四極管と 3 D 印刷可能なハイパー ドライブの建設を提案します。ハイパー ドライブの故障は、ラットを数週間の期間にわたって自由に行動で脳の活動を記録するために設計されています。

ビデオ: Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

Construction of Microdrive Arrays for Chronic Neural Recordings in Awake Behaving Mice

State-of-the-art electrophysiological recordings from the brains of freely behaving animals allow researchers to simultaneously examine local field potentials (LFPs) from populations of neurons and action potentials from individual cells, as the animal engages in experimentally relevant tasks. Chronically implanted microdrives allow for brain recordings to last over periods of several weeks. Miniaturized drives and lightweight components allow for these long-term recordings to occur in small mammals, such as mice. By using tetrodes, which consist of tightly braided bundles of four electrodes in which each wire has a diameter of 12.5 &mu;m, it is possible to isolate physiologically active neurons in superficial brain regions such as the cerebral cortex, dorsal hippocampus, and subiculum, as well as deeper regions such as the striatum and the amygdala. Moreover, this technique insures stable, high-fidelity neural recordings as the animal is challenged with a variety of behavioral tasks. This manuscript describes several techniques that have been optimized to record from the mouse brain. First, we show how to fabricate tetrodes, load them into driveable tubes, and gold-plate their tips in order to reduce their impedance from M&Omega; to K&Omega; range. Second, we show how to construct a custom microdrive assembly for carrying and moving the tetrodes vertically, with the use of inexpensive materials. Third, we show the steps for assembling a commercially available microdrive (Neuralynx VersaDrive) that is designed to carry independently movable tetrodes. Finally, we present representative results of local field potentials and single-unit signals obtained in the dorsal subiculum of mice. These techniques can be easily modified to accommodate different types of electrode arrays and recording schemes in the mouse brain.

The design and assembly of microdrives for in vivo electrophysiological recordings of brain signals from the mouse is described. By attaching microelectrode bundles to sturdy driveable carriers, these techniques allow for long-term and stable neural recordings. The lightweight design allows for unrestricted behavioral performance by the animal following drive implantation.

アウェイク行動マウスにおける慢性神経録音のためにマイクロドライブアレイの構築

State-of-the-art electrophysiological recordings from the brains of freely behaving animals allow researchers to simultaneously examine local field ...

行動学

Combining Behavioral Endocrinology and Experimental Economics: Testosterone and Social Decision Making

Behavioral endocrinological research in humans as well as in animals suggests that testosterone plays a key role in social interactions. Studies in rodents have shown a direct link between testosterone and aggressive behavior1 and folk wisdom adapts these findings to humans, suggesting that testosterone induces antisocial, egoistic or even aggressive behavior2. However, many researchers doubt a direct testosterone-aggression link in humans, arguing instead that testosterone is primarily involved in status-related behavior3,4. As a high status can also be achieved by aggressive and antisocial means it can be difficult to distinguish between anti-social and status seeking behavior.
We therefore set up an experimental environment, in which status can only be achieved by prosocial means. In a double-blind and placebo-controlled experiment, we administered a single sublingual dose of 0.5 mg of testosterone (with a hydroxypropyl-&beta;-cyclodextrin carrier) to 121 women and investigated their social interaction behavior in an economic bargaining paradigm. Real monetary incentives are at stake in this paradigm; every player A receives a certain amount of money and has to make an offer to another player B on how to share the money. If B accepts, she gets what was offered and player A keeps the rest. If B refuses the offer, nobody gets anything. A status seeking player A is expected to avoid being rejected by behaving in a prosocial way, i.e. by making higher offers.
The results show that if expectations about the hormone are controlled for, testosterone administration leads to a significant increase in fair bargaining offers compared to placebo. The role of expectations is reflected in the fact that subjects who report that they believe to have received testosterone make lower offers than those who say they believe that they were treated with a placebo. These findings suggest that the experimental economics approach is sensitive for detecting neurobiological effects as subtle as those achieved by administration of hormones. Moreover, the findings point towards the importance of both psychosocial as well as neuroendocrine factors in determining the influence of testosterone on human social behavior.

The procedure described in this protocol shows that testosterone administration and folk beliefs about testosterone may be associated with directly opposed social behaviors.

行動内分泌学と実験経済学を組み合わせること：テストステロンと社会的意思決定

Behavioral endocrinological research in humans as well as in animals suggests that testosterone plays a key role in social interactions. Studies in ...

神経科学

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of behaviors from the spiking activity of its neurons. One of the most effective methods to monitor spiking activity from a large number of neurons in multiple local neuronal circuits simultaneously is by using silicon electrode arrays1-3.
Action potentials produce large transmembrane voltage changes in the vicinity of cell somata. These output signals can be measured by placing a conductor in close proximity of a neuron. If there are many active (spiking) neurons in the vicinity of the tip, the electrode records combined signal from all of them, where contribution of a single neuron is weighted by its 'electrical distance'. Silicon probes are ideal recording electrodes to monitor multiple neurons because of a large number of recording sites (+64) and a small volume. Furthermore, multiple sites can be arranged over a distance of millimeters, thus allowing for the simultaneous recordings of neuronal activity in the various cortical layers or in multiple cortical columns (Fig. 1). Importantly, the geometrically precise distribution of the recording sites also allows for the determination of the spatial relationship of the isolated single neurons4. Here, we describe an acute, large-scale neuronal recording from the left and right forelimb somatosensory cortex simultaneously in an anesthetized rat with silicon probes (Fig. 2).

Extracellular recordings of neuronal activity using silicon probes in the anesthetized rat will be described. This technique allows information to be obtained across multiple brain areas from more than 100 neurons simultaneously. It provides information with single cell resolution about neuronal ensembles dynamics in multiple local circuits.

麻酔ラットのシリコンプローブで大規模なニューロンアンサンブルの録音

Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of ...

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

In vivo electrophysiology is a powerful technique to investigate the relationship between brain activity and behavior at a millisecond and micrometer scale. However, current methods mostly rely on tethered cable recordings or only use unidirectional systems, allowing either recording or stimulation of neural activity, but not at the same time or same target. Here, a new wireless, bidirectional device for simultaneous multichannel recording and stimulation of neural activity in freely behaving rats is described. The system operates through a single portable head stage that both transmits recorded activity and can be targeted in real-time for brain stimulation using a telemetry-based multichannel software. The head stage is equipped with a preamplifier and a rechargeable battery, allowing stable long-term recordings or stimulation for up to 1 h. Importantly, the head stage is compact, weighs 12 g (including battery) and thus has minimal impact on the animal&#180;s behavioral repertoire, making the method applicable to a broad set of behavioral tasks. Moreover, the method has the major advantage that the effect of brain stimulation on neural activity and behavior can be measured simultaneously, providing a tool to assess the causal relationships between specific brain activation patterns and behavior. This feature makes the method particularly valuable for the field of deep brain stimulation, allowing precise assessment, monitoring, and adjustment of stimulation parameters during long-term behavioral experiments. The applicability of the system has been validated using the inferior colliculus as a model structure.

A Wireless, Bidirectional Interface for In Vivo Recording and Stimulation of Neural Activity in Freely Behaving Rats

A wireless, bidirectional system for multi-channel neural recordings and stimulation in freely behaving rats is introduced. The system is light and compact, thus having minimal impact on the animal&#180;s behavioral repertoire. Moreover, this bidirectional system provides a sophisticated tool to assess causal relationships between brain activation patterns and behavior.

ワイヤレス、 In Vivo記録そして自由行動下ラットにおける神経活動の刺激のための双方向インタ フェース

In vivo electrophysiology is a powerful technique to investigate the relationship between brain activity and behavior at a millisecond and micrometer ...

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of neurons and circuits requires methods with the spatial selectivity and temporal resolution appropriate for mechanistic analysis of neural ensembles in the behaving animal, i.e. recording of representatively large samples of isolated single neurons. Ensemble monitoring of neuronal activity has progressed remarkably in the past decade in both small and large-brained animals, including human subjects1-11. Multiple-site recording with silicon-based devices are particularly effective because of their scalability, small volume and geometric design.
Here, we describe methods for recording multiple single neurons and local field potential in behaving rodents, using commercially available micro-machined silicon probes with custom-made accessory components. There are two basic options for interfacing silicon probes to preamplifiers: printed circuit boards and flexible cables. Probe supplying companies (<a href="http://www.neuronexustech.com/" >http://www.neuronexustech.com/</a>; <a href="http://www.sbmicrosystems.com/">http://www.sbmicrosystems.com/</a>; <a href="http://www.acreo.se/">http://www.acreo.se/</a>) usually provide the bonding service and deliver probes bonded to printed circuit boards or flexible cables. Here, we describe the implantation of a 4-shank, 32-site probe attached to flexible polyimide cable, and mounted on a movable microdrive. Each step of the probe preparation, microdrive construction and surgery is illustrated so that the end user can easily replicate the process.

We describe methods for large-scale recording of multiple single units and local field potential in behaving rodents with silicon probes. Drive fabrication, probe attachment to the drive and probe implantation processes are illustrated in sufficient details for easy replication.

動作げっ歯類の可動シリコンプローブによるニューロンの大規模なレコーディング

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. Lightweight recording devices carrying a high density array of tetrodes allow for the simultaneous monitoring of the activity of tens to hundreds of individual neurons. Here we describe a protocol for the fabrication of a micro-drive array with twenty one independently movable micro-drives. This device has been used successfully to record from hippocampal and cortical neurons in our lab. We show how to prepare a custom designed, 3-D printed plastic base that will hold the micro-drives. We demonstrate how to construct the individual micro-drives and how to assemble the complete micro-drive array. Further preparation of the drive array for surgical implantation, such as the fabrication of tetrodes, loading of tetrodes into the drive array and gold-plating, is covered in a subsequent video article.

Micro-drive Array for Chronic in vivo Recording: Drive Fabrication

In this protocol we demonstrate how to fabricate a micro-drive array for chronic electrophysiological recordings in rats.

慢性のためのマイクロドライブアレイ in vivoで録音：ドラ​​イブの作製

Chronic recording of large populations of neurons is a valuable technique for studying the function of neuronal circuits in awake behaving rats. ...

生物学

Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential amplitude of action potential signatures over the channels of a tetrode allows for the isolation of single-unit activity from multi-unit signals.  The ability to precisely control the stereotaxic location and depth of the tetrode is critical for studying coordinated neural activity across brain regions.  In combination with a micro-drive array, it is possible to achieve precise placement and stable control of many tetrodes over the course of days to weeks.  In this protocol, we demonstrate how to fabricate and condition tetrodes using basic tools and materials, install the tetrodes into a multi-drive tetrode array for chronic in-vivo recording in the rat, make ground wire connections to the micro-drive array, and attach a protective cone onto the micro-drive array in order to protect the tetrodes from physical contact with the environment.



Micro-drive Array for Chronic in vivo Recording: Tetrode Assembly

In this protocol we demonstrate how to fabricate and condition tetrodes for use with a micro-drive array, which was designed for chronic electrophysiological recordings in rats. In addition, we illustrate the final stages of micro-drive array construction, which includes installing ground wires and a protective cone.

慢性のためのマイクロドライブアレイ in vivoで録音：四極管アセンブリ

The tetrode, a bundle of four electrodes, has proven to be a valuable tool for the simultaneous recording of multiple neurons in-vivo.  The differential ...

Hybrid Microdrive System with Recoverable Opto-Silicon Probe and Tetrode for Dual-Site High Density Recording in Freely Moving Mice

Multi-regional neural recordings can provide crucial information to understanding fine-timescale interactions between multiple brain regions. However, conventional microdrive designs often only allow use of one type of electrode to record from single or multiple regions, limiting the yield of single-unit or depth profile recordings. It also often limits the ability to combine electrode recordings with optogenetic tools to target pathway and/or cell type specific activity. Presented here is a hybrid microdrive array for freely moving mice to optimize yield and a description of its fabrication and reuse of the microdrive array. The current design employs nine tetrodes and one opto-silicon probe implanted in two different brain areas simultaneously in freely moving mice. The tetrodes and the opto-silicon probe are independently adjustable along the dorsoventral axis in the brain to maximize the yield of unit and oscillatory activities. This microdrive array also incorporates a set-up for light, mediating optogenetic manipulation to investigate the regional- or cell type-specific responses and functions of long-range neural circuits. In addition, the opto-silicon probe can be safely recovered and reused after each experiment. Because the microdrive array consists of 3D-printed parts, the design of microdrives can be easily modified to accommodate various settings. First described is the design of the microdrive array and how to attach the optical fiber to a silicon probe for optogenetics experiments, followed by fabrication of the tetrode bundle and implantation of the array into a mouse brain. The recording of local field potentials and unit spiking combined with optogenetic stimulation also demonstrate feasibility of the microdrive array system in freely moving mice.

This protocol describes the construction of a hybrid microdrive array that allows implantation of nine independently adjustable tetrodes and one adjustable opto-silicon probe in two brain regions in freely moving mice. Also demonstrated is a method for safely recovering and reusing the opto-silicon probe for multiple purposes.

自由に動くマウスの二重部位高密度記録のための回収可能なオプトシリコンプローブとテトロードを備えたハイブリッドマイクロドライブシステム

Multi-regional neural recordings can provide crucial information to understanding fine-timescale interactions between multiple brain regions. However, ...

若年マウスの日常神経活動を記録するためのマイクロドライブインプラント

A Lightweight Drive Implant for Chronic Tetrode Recordings in Juvenile Mice

In vivo electrophysiology provides unparalleled insight into the sub-second-level circuit dynamics of the intact brain and represents a method of particular importance for studying mouse models of human neuropsychiatric disorders. However, such methods often require large cranial implants, which cannot be used in mice at early developmental time points. As such, virtually no studies of in vivo physiology have been performed in freely behaving infant or juvenile mice, despite the fact that a better understanding of neurological development in this critical window would likely provide unique insights into age-dependent developmental disorders such as autism or schizophrenia. Here, a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy are described that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in mice as they age from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond, a time window roughly corresponding to the human ages of 2 years old through to adulthood. The number of recording electrodes and final recording sites can be easily modified and expanded, thus allowing flexible experimental control of the in vivo monitoring of behavior- or disease-relevant brain regions across development.

著者スポットライト:若年マウスの慢性四極管記録のための軽量ドライブインプラント  

Here, we describe a micro-drive design, surgical implantation procedure, and post-surgery recovery strategy that allow for chronic field and single-unit recordings from multiple brain regions simultaneously in juvenile and adolescent mice across a critical developmental window from postnatal day 20 (p20) to postnatal day 60 (p60) and beyond.

ラットの行動で大規模な神経細胞記録向上のマルチ・テトロードハイパードライブの構築

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さらに動画を探す

この動画の章

アウェイク行動マウスにおける慢性神経録音のためにマイクロドライブアレイの構築

行動内分泌学と実験経済学を組み合わせること：テストステロンと社会的意思決定

麻酔ラットのシリコンプローブで大規模なニューロンアンサンブルの録音

ワイヤレス、 In Vivo記録そして自由行動下ラットにおける神経活動の刺激のための双方向インタフェース

動作げっ歯類の可動シリコンプローブによるニューロンの大規模なレコーディング

慢性のためのマイクロドライブアレイ in vivoで録音：ドライブの作製

慢性のためのマイクロドライブアレイ in vivoで録音：四極管アセンブリ

自由に動くマウスの二重部位高密度記録のための回収可能なオプトシリコンプローブとテトロードを備えたハイブリッドマイクロドライブシステム

幼若マウスにおける慢性四尾行記録のための軽量駆動インプラント

幼若マウスにおける慢性四尾行記録のための軽量駆動インプラント

ラットの行動で大規模な神経細胞記録向上のマルチ ・ テトロード ハイパー ドライブの構築

要約

さらに動画を探す

この動画の章

アウェイク行動マウスにおける慢性神経録音のためにマイクロドライブアレイの構築

行動内分泌学と実験経済学を組み合わせること：テストステロンと社会的意思決定

麻酔ラットのシリコンプローブで大規模なニューロンアンサンブルの録音

ワイヤレス、 In Vivo記録そして自由行動下ラットにおける神経活動の刺激のための双方向インタ フェース

動作げっ歯類の可動シリコンプローブによるニューロンの大規模なレコーディング

慢性のためのマイクロドライブアレイ in vivoで録音：ドラ​​イブの作製

慢性のためのマイクロドライブアレイ in vivoで録音：四極管アセンブリ

自由に動くマウスの二重部位高密度記録のための回収可能なオプトシリコンプローブとテトロードを備えたハイブリッドマイクロドライブシステム

幼若マウスにおける慢性四尾行記録のための軽量駆動インプラント

幼若マウスにおける慢性四尾行記録のための軽量駆動インプラント

ラットの行動で大規模な神経細胞記録向上のマルチ・テトロードハイパードライブの構築

ワイヤレス、 In Vivo記録そして自由行動下ラットにおける神経活動の刺激のための双方向インタフェース

慢性のためのマイクロドライブアレイ in vivoで録音：ドライブの作製