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JoVE Journal

Chemistry

This content is Open Access.

Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients

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08:15 min

July 16th, 2018

July 16th, 2018

7,741 Views

0:04

Title

0:41

Preparation of Small Lipid Vesicles

2:38

Preparation of GUV-MLV Complexes

4:12

Micropipette Preparation and Microinjection

6:09

Formation and Translation of Membrane Tubular Protrusions (MTPs) using the Calcium Ion Source

6:57

Results: Formation of Membrane Tubular Protrusions in Giant Vesicles Upon Localized Application of Calcium Ion Gradients

7:37

Conclusion

文字起こし

We present a technique for contactless micromanipulation of vesicles, using localized calcium ion gradients. Microinjection of a calcium ion solution, in the vicinity of a giant lipid vesicle, is utilized to remodel the lipid membrane, resulting in the production of membrane tubular protrusions.

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