Name: multiscale structures aggregated by imprinted nanofibers for functional surfaces
Uploaded: 2018-09-11T14:45:00.000+00:00
Duration: 6 min 14 s
Description: Watch this Scientific Journal Video about Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces at JoVE.com

この資料は基本的な科学研究開発プログラムを通じて、国立研究財団の韓国 (NRF) 科学省、ICT と将来計画 (NRF 2017R1A2B4008053)、エネルギー、産業貿易省 (によって資金を供給によってサポートされる作業に基づいてください。MOTIE、韓国) [産業技術革新プログラム ナンバー 10052802 と経済協力部 (N0002310) 産業奨励プログラムを通じて技術 (KIAT) 振興院。

1. AAO フィルター (図 1) を用いたナノ ・ マイクロ スケールの構造面の製作
<ol><li>細孔径、高さ、および 200 nm、60 μ m、25 mm の直径を持つ AAO フィルターをそれぞれ購入します。</li>
	<li>1.2. 5 分間 99.8% と 99.9% のイソプロピル アルコール (IPA) アセトンを使用する 100 μ m の厚さを有するポリエチレン ポリエチレンテレフタ レート (PET) フィルムの表面をきれいに、3 分間空気銃を使用して完全に乾いて。</li>
	<li>汚染物質がなく平らな表面に PET フィルムを置き、UV 硬化型ポリウレタン アクリル酸 (PUA) の 0.1 mL のドロップを追加-樹脂表面に 257.4 cPs の粘度を入力します。</li>
	<li>樹脂に AAO フィルターを置き、ゴム製ローラーを使用して、32 mm の直径を均一に、押します。ローラ プッシュする必要が繰り返し、慎重に押したとき、樹脂の広がりは目視です。 注意: AAO フィルターは脆性であり、過度の力が加わる場合を破る可能性があります。</li>
	<li>圧延後、365 の波長の紫外線に (樹脂を使用してアタッチ) ペットと貝割のフィルターで作られた標本を公開 30 nm 樹脂を硬化します。</li>
	<li>フィルターを溶解する 10 分間 2 M NaOH 溶液 100 mL に養生供試体を浸します。 注: SEM 画像表示断面と表面構造 (図 2)。</li>
	<li>DI 水で試料をきれいし、完全に空気銃を使用して 3 分間乾燥します。 注: Na とアルが検出されないと、完全に EDX 分析を確認した (図 3) をエッチングします。</li>
</ol>2. 表面処理
<ol><li>UV オゾン処理</li>
	<li>3 分間の空気銃を使用してそれを乾燥し、5 分 IPA と DI 水を用いたナノ ・ マイクロ スケール構造を持つ試料をきれい。</li>
	<li>供試体 (マルチ スケール構造を持つ側) のマルチ スケール構造の側を照射紫外線 (波長 185-254 nm) を用いた 60 分。 メモ: UV オゾン装置 25 mW/cm2の強度があります。</li>
	<li>オクタデシルトリクロロシラン (OTS) 自己集合</li>
	<li>グローブ ボックス内のホット プレートを置き、気相堆積プロセスのための N2環境を維持します。</li>
	<li>ガラスに粘着テープを用いた平板試料の端を修正します。グラスやプレートのサイズが (8 mm 径、高さの 13 mm) でビーカーの上部をカバーするのに十分な大きさであることを確認します。</li>
	<li>5"x 7"とホット プレートにビーカーを置き、OTS 溶液 2 mL をピペットを使用してビーカーに加えます。</li>
	<li>試料をビーカーに下向きの顔に、板やガラスのビーカーをカバーします。</li>
	<li>100 ° C、それから削除グローブ ボックスからの標本で 60 分のためのプロセス。 注: OTS コーティング プロセスの後、ビーカーとグローブ ボックスする必要があります洗浄します。</li>
</ol>3. 潤滑剤を注入することによる機能表面の作製
<ol><li>預金約 0.2 mL OTS コーティングにパーフルオロ カーボン (PFC) 液体の自己のナノファイバーを集約。</li>
	<li>光学顕微鏡対物レンズを用いた 5 X-20 X で PFC の湿潤過程を観察します。</li>
	<li>垂直方向の位置にサンプルを数時間置くことによって余分な PFC の液体を削除します。</li>
</ol>

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著者は開示する競合金融興味を持ってないです。

自己集計のナノファイバーの作製の重要なステップは、ゴムのローラーで樹脂を適用するとき脆性 AAO フィルターは壊れないことを確認することです。実際には、AAO フィルターがエッチングのステップの前に任意の時点で壊れないが確保されなければなりません。AAO フィルターは直径 25 mm、基板のサイズは約 30 × 30 mm です。
自己集計ナノファイバー適切な表面処理を通...

ナノスケール テクスチャ構造ナノ粒子・ カーボンナノ チューブ、ナノファイバーは、科学界で注目を集めているなど、電気、医療、光学、表面を含むさまざまなアプリケーションにユニークな特性を実証1,2,3,4,5,6,7、8をエンジニア リングします。特に、ナノファイバー、伸縮自在かつ透明な電極9、ウェアラブル センサー10,11、配線12,13, ナノ光学アプリケーションで広く14. 自己ゾル-ゲル法などのナノスケール構造体を作製、リソグラフィとレプリケーション15,16,17,18、さまざまな方法の中で 19、20テンプレートを使用して直接レプリケーション現在考えられている有望な方法それは簡単な費用効率、および様々 な硬化材料21,22に該当するので,23,24,25,26。
多数のナノ細孔とマイクロ スケールの高さを持つマルチ スケール構造により AAO として広く利用されて、テンプレート ナノファイバー ・高アスペクト比27,28,29とナノチューブの作製,30します。 ただし、このような高アスペクト比で表面張力のためナノファイバーは、31,32,33を簡単に集計する傾向が。既存研究は、集計33,34せず個別に分離比 5:1 未満を有するものがある一方、アスペクト比 15:1 より大きいを有するナノファイバーが直立せずは、代わりに、集計を証明しています。毛管力と表面張力によるエッチング、ナノファイバー作製中にプロセスの 1 つであるアルミナの除去時に重要な役割を果たします。アスペクト比が増加すると、ナノファイバーの表面張力集計を引き起こして、互いに近い方のそれらを引く傾向があります。いくつかの研究は、高分子・金属ナノファイバーで特に観察される集計35にそのようなを防止する方法に焦点を当てています。これらのうち、ナノファイバーの表面の水和は液体は、ナノファイバーの間のスペースを占めている、表面張力が低下するため、集積を減らすことができます。さらに、凍結乾燥法はナノファイバーの表面張力を減少させることによってまた集計を減らす可能性があります。しかし、様々 な努力にもかかわらず挑戦のまま高アスペクト比を有するナノファイバーの矯正です。
このためには、肯定的な方法で凝集現象を利用してもつれたナノファイバーのマルチ スケール構造を製造するユニークな方法を報告します。ここ、ナノファイバー構造は AAO フィルターとポリウレタン アクリル (PUA) を使用して刻印される-257.4 の粘度の樹脂を型 cP。UV ナノインプリントリソグラフィ (UV-NIL) が実行された後、金型は NaOH 溶液でエッチングされています。提案のマルチ スケール構造を特徴付ける、自己組織化単分子膜のコーティングなど適切な表面処理、UV オゾン処理後集計ナノファイバーと表面濡れ性のサンプルのパターン行動を調査します。.さらに、マルチ スケールの多孔性の表面を潤滑剤注入プロセスを使用して滑りやすい表面に単に変換できることを提案します。

<table><tbody><tr><td>MINS 511RM</td><td>Minuta Tech</td><td></td><td>UV curable resin</td></tr><tr><td>Octadecyltrichlorosilane (OTS)</td><td>Aldrich</td><td></td><td>Surface treatment</td></tr><tr><td>Sodium oxidanide</td><td>SAMCHUN</td><td></td><td>Etching solution</td></tr><tr><td>Anopore Inoganic Membranes</td><td>Whatman</td><td></td><td>25mm/0.2&amp;micro;m</td></tr><tr><td>MT-UV-A 47</td><td>Meiji Techno</td><td></td><td>UV curing equipment</td></tr><tr><td>UVC-30</td><td>Jaesung Engineering</td><td></td><td>UVO treatment equipment</td></tr><tr><td>Smart Drop Plus</td><td>FEMTOFAB</td><td></td><td>Contact angle measurement</td></tr><tr><td>Fluorinert FC-70</td><td>3M</td><td></td><td>liquid mixture of completely fluorinated aliphatic compounds</td></tr><tr><td>Polyethylene terephthalate film</td><td>Sunchem</td><td></td><td>Substrate</td></tr><tr><td>Acetone (99.8%)</td><td>Daejung</td><td></td><td>Cleaning solution</td></tr><tr><td>Isopropyl alcohol (99.9%)</td><td>Daejung</td><td></td><td>Cleaning solution</td></tr><tr><td>Rubber roller</td><td>Hwahong</td><td></td><td>For application of resin</td></tr><tr><td>Corning Stirring Hot Plates</td><td>Corning</td><td></td><td>Hot plate equipment (5&quot; x 7&quot;)</td></tr></tbody></table>

multiscale structures aggregated by imprinted nanofibers for functional surfaces

マルチ スケール表面構造は、表面にいくつかの潜在的なアプリケーションのための関心の高まりを集めています。ただし、フィールドの既存の挑戦は安易な低コスト、および高スループット法を用いたハイブリッド マイクロ ・ ナノ構造の作製です。これらの課題を克服するためには、今回は、アルミニウムの陽極酸化 (AAO) フィルターとインプリント プロセスのみとナノファイバーの蒸発の自己凝集過程を用いたマルチ スケール構造を作製するためのプロトコルを提案します。ナノファイバーをまっすぐに目指した以前の試みとは異なり高アスペクト比のスケールの集約されたナノファイバーの独特な製作法を紹介します。さらに、表面形態とさまざまな液体にこれらの構造体の濡れ性は多機能表面での使用を容易にするために調べた。

高速でシンプルなインプリント金型として AAO フィルターを用いた多重スケール ナノ ・ マイクロ ハイブリッド構造の作製法を示しました。全体のプロセスは 30 分 (図 4) を取った。これは、水酸化ナトリウムを用いたエッチング プロセスを経て、結果として生じる表面展示集計ナノファイバーの表面張力によって引き起こされるため、元の AAO ...

Watch this Scientific Journal Video about Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces at JoVE.com

Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces

マルチ スケール構造機能表面の刻印ナノファイバーによる集計

発表は、陽極酸化アルミニウム フィルターを用いて作製したナノファイバーを集約することによって機能性表面のナノ ・ マイクロ スケール構造を作製する簡単な方法です。

Multiscale surface structures have attracted increasing interest owing to several potential applications in surface devices. However, an existing ...

multiscale-structures-aggregated-imprinted-nanofibers-for-functional

Research

JoVE Journal

Engineering

6.5K ビュー.  Changwon National University. 発表は、陽極酸化アルミニウム フィルターを用いて作製したナノファイバーを集約することによって機能性表面のナノ ・ マイクロ スケール構造を作製する簡単な方法です。

ビデオ: Multiscale Structures Aggregated by Imprinted Nanofibers for Functional Surfaces

Nanomoulding of Functional Materials, a Versatile Complementary Pattern Replication Method to Nanoimprinting

We describe a nanomoulding technique which allows low-cost nanoscale patterning of functional materials, materials stacks and full devices. Nanomoulding combined with layer transfer enables the replication of arbitrary surface patterns from a master structure onto the functional material. Nanomoulding can be performed on any nanoimprinting setup and can be applied to a wide range of materials and deposition processes. In particular we demonstrate the fabrication of patterned transparent zinc oxide electrodes for light trapping applications in solar cells.

We describe a nanomoulding technique which allows low-cost nanoscale patterning of functional materials, materials stacks and full devices. Nanomoulding can be performed on any nanoimprinting setup and can be applied to a wide range of materials and deposition processes.

機能材料のNanomoulding、ナノインプリントへ多目的相補パターンレプリケーション方法

We describe a nanomoulding technique which  allows low-cost nanoscale patterning of functional materials, materials stacks  and full devices. Nanomoulding ...

工学

Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration

Microstructured composite beams reinforced with complex three-dimensionally (3D) patterned nanocomposite microfilaments are fabricated via nanocomposite in&#64257;ltration of 3D interconnected microfluidic networks. The manufacturing of the reinforced beams begins with the fabrication of microfluidic networks, which involves layer-by-layer deposition of fugitive ink filaments using a dispensing robot, filling the empty space between filaments using a low viscosity resin, curing the resin and finally removing the ink.&#160;Self-supported 3D structures with other geometries and many layers (e.g.&#160;a few hundreds layers) could be built using this method. The resulting tubular micro&#64258;uidic networks are then infiltrated with thermosetting nanocomposite suspensions containing nanofillers (e.g.&#160;single-walled carbon nanotubes), and subsequently cured. The infiltration is done by applying a pressure gradient between two ends of the empty network (either by applying a vacuum or vacuum-assisted microinjection). Prior to the infiltration, the nanocomposite suspensions are prepared by dispersing nanofillers into polymer matrices using ultrasonication and three-roll mixing methods. The nanocomposites (i.e.&#160;materials infiltrated) are then solidified under UV exposure/heat cure, resulting in a 3D-reinforced composite structure. The technique presented here enables the design of functional nanocomposite macroscopic products for microengineering applications such as actuators and sensors.

Three-dimensional (3D) microstructured composite beams are fabricated through the directed and localized infiltration of nanocomposites into 3D porous microfluidic networks. The flexibility of this manufacturing method enables the utilization of different thermosetting materials and nanofillers in order to achieve a variety of functional 3D reinforced nanocomposite macroscopic products.

マイクロ流体浸透を通じて立体的微細構造ナノ複合材料の製造

Microstructured composite beams reinforced with complex three-dimensionally (3D) patterned nanocomposite microfilaments are fabricated via nanocomposite ...

化学

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

The extracellular matrix (ECM) in tissues is synthesized and assembled by cells to form a 3D fibrillar, protein network with tightly regulated fiber diameter, composition and organization. In addition to providing structural support, the physical and chemical properties of the ECM play an important role in multiple cellular processes including adhesion, differentiation, and apoptosis. In vivo, the ECM is assembled by exposing cryptic self-assembly (fibrillogenesis) sites within proteins. This process varies for different proteins, but fibronectin (FN) fibrillogenesis is well-characterized and serves as a model system for cell-mediated ECM assembly. Specifically, cells use integrin receptors on the cell membrane to bind FN dimers and actomyosin-generated contractile forces to unfold and expose binding sites for assembly into insoluble fibers. This receptor-mediated process enables cells to assemble and organize the ECM from the cellular to tissue scales. Here, we present a method termed surface-initiated assembly (SIA), which recapitulates cell-mediated matrix assembly using protein-surface interactions to unfold ECM proteins and assemble them into insoluble fibers. First, ECM proteins are adsorbed onto a hydrophobic polydimethylsiloxane (PDMS) surface where they partially denature (unfold) and expose cryptic binding domains. The unfolded proteins are then transferred in well-defined micro- and nanopatterns through microcontact printing onto a thermally responsive poly(N-isopropylacrylamide) (PIPAAm) surface. Thermally-triggered dissolution of the PIPAAm leads to final assembly and release of insoluble ECM protein nanofibers and nanostructures with well-defined geometries. Complex architectures are possible by engineering defined patterns on the PDMS stamps used for microcontact printing. In addition to FN, the SIA process can be used with laminin, fibrinogen and collagens type I and IV to create multi-component ECM nanostructures. Thus, SIA can be used to engineer ECM protein-based materials with precise control over the protein composition, fiber geometry and scaffold architecture in order to recapitulate the structure and composition of the ECM in vivo.

A method to obtain nanofibers and complex nanostructures from single or multiple extracellular matrix proteins is described. This method uses protein-surface interactions to create free-standing protein-based materials with tunable composition and architecture for use in a variety of tissue engineering and biotechnology applications.

表面開始アセンブリの使用ECMタンパク質のナノファイバーおよびナノ構造エンジニア

The extracellular matrix (ECM) in tissues is synthesized and assembled by cells to form a 3D fibrillar, protein network with tightly regulated fiber ...

生物工学

Fabricating Reactive Surfaces with Brush-like and Crosslinked Films of Azlactone-Functionalized Block Co-Polymers

In this paper, fabrication methods that generate novel surfaces using the azlactone-based block co-polymer, poly (glycidyl methacrylate)-block-poly (vinyl dimethyl azlactone) (PGMA-b-PVDMA), are presented. Due to the high reactivity of azlactone groups towards amine, thiol, and hydroxyl groups, PGMA-b-PVDMA surfaces can be modified with secondary molecules to create chemically or biologically functionalized interfaces for a variety of applications. Previous reports of patterned PGMA-b-PVDMA interfaces have used traditional top-down patterning techniques that generate non-uniform films and poorly controlled background chemistries. Here, we describe customized patterning techniques that enable precise deposition of highly uniform PGMA-b-PVDMA films in backgrounds that are chemically inert or that have biomolecule-repellent properties. Importantly, these methods are designed to deposit PGMA-b-PVDMA films in a manner that completely preserves azlactone functionality through each processing step. Patterned films show well-controlled thicknesses that correspond to polymer brushes (~90 nm) or to highly crosslinked structures (~1-10 &#956;m). Brush patterns are generated using either the parylene lift-off or interface directed assembly methods described and are useful for precise modulation of overall chemical surface reactivity by adjusting either the PGMA-b-PVDMA pattern density or the length of the VDMA block. In contrast, the thick, crosslinked PGMA-b-PVDMA patterns are obtained using a customized micro-contact printing technique and offer the benefit of higher loading or capture of secondary material due to higher surface area to volume ratios. Detailed experimental steps, critical film characterizations, and trouble-shooting guides for each fabrication method are discussed.

Surface fabrication methods for patterned deposition of nanometer thick brushes or micron thick, crosslinked films of an azlactone block co-polymer are reported. Critical experimental steps, representative results, and limitations of each method are discussed. These methods are useful for creating functional interfaces with tailored physical features and tunable surface reactivity.

ブラシのような反応性表面を加工し、アズラクトン官能基化ブロック共重合体の架橋膜

In this paper, fabrication methods that generate novel surfaces using the azlactone-based block co-polymer, poly (glycidyl methacrylate)-block-poly (vinyl ...

Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization

The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible applications in controlling cell morphology/orientation. Nanofiber organization is controlled with a new fabrication apparatus that enables the gradual decrease of fiber organization in a scaffold. Changing the alignment of fibers is achieved through decreasing deposition time of random electrospun fibers on a uniaxially aligned fiber mat. By covering the collector with a moving barrier/mask, along the same axis as fiber deposition, the organizational structure is easily controlled. For tissue engineering purposes, adipose-derived stem cells can be seeded to these scaffolds. Stem cells undergo morphological changes as a result of their position on the varied organizational structure, and can potentially differentiate into different cell types depending on their locations. Additionally, the graded organization of fibers enhances the biomimicry of nanofiber scaffolds so they more closely resemble the natural orientations of collagen nanofibers at tendon-to-bone insertion site compared to traditional scaffolds. Through nanoencapsulation, the gradated fibers also afford the possibility to construct chemical gradients in fiber scaffolds, and thereby further strengthen their potential applications in fast screening of cell-materials interaction and interfacial tissue regeneration. This technique enables the production of continuous gradient scaffolds, but it also can potentially produce fibers in discrete steps by controlling the movement of the moving barrier/mask in a discrete fashion.

Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.

繊維組織における階調の電界紡糸ナノファイバー足場

The goal of this protocol is to report a simple method for generating nanofiber scaffolds with gradations in fiber organization and test their possible ...

Expanding Nanopatterned Substrates Using Stitch Technique for Nanotopographical Modulation of Cell Behavior

Substrate nanotopography has been shown to be a potent modulator of cell phenotype and function. To dissect nanotopography modulation of cell behavior, a large area of nanopatterned substrate is desirable so that enough cells can be cultured on the nanotopography for subsequent biochemical and molecular biology analyses. However, current nanofabrication techniques have limitations to generate highly defined nanopatterns over a large area. Herein, we present a method to expand nanopatterned substrates from a small, highly defined nanopattern to a large area using stitch technique. The method combines multiple techniques, involving soft lithography to replicate poly(dimethylsiloxane) (PDMS) molds from a well-defined mold, stitch technique to assemble multiple PDMS molds to a single large mold, and nanoimprinting to generate a master mold on polystyrene (PS) substrates. With the PS master mold, we produce PDMS working substrates and demonstrate nanotopographical modulation of cell spreading. This method provides a simple, affordable yet versatile avenue to generate well-defined nanopatterns over large areas, and is potentially extended to create micro-/nanoscale devices with hybrid components.

A protocol for producing a large area of nanopatterned substrate from small nanopatterned molds for study of nanotopographical modulation of cell behavior is presented.

細胞の挙動のNanotopographical変調のステッチ法を用いたナノパターン基板を拡大

Substrate nanotopography has been shown to be a potent modulator of cell phenotype and function. To dissect nanotopography modulation of cell behavior, a ...

Fabrication of a Multiplexed Artificial Cellular MicroEnvironment Array

Cellular microenvironments consist of a variety of cues, such as growth factors, extracellular matrices, and intercellular interactions. These cues are well orchestrated and are crucial in regulating cell functions in a living system. Although a number of researchers have attempted to investigate the correlation between environmental factors and desired cellular functions, much remains unknown. This is largely due to the lack of a proper methodology to mimic such environmental cues in vitro, and simultaneously test different environmental cues on cells. Here, we report an integrated platform of microfluidic channels and a nanofiber array, followed by high-content single-cell analysis, to examine stem cell phenotypes altered by distinct environmental factors. To demonstrate the application of this platform, this study focuses on the phenotypes of self-renewing human pluripotent stem cells (hPSCs). Here, we present the preparation procedures for a nanofiber array and the microfluidic structure in the fabrication of a Multiplexed Artificial Cellular MicroEnvironment (MACME) array. Moreover, overall steps of the single-cell profiling, cell staining with multiple fluorescent markers, multiple fluorescence imaging, and statistical analyses, are described.

This article describes the detailed methodology to prepare a Multiplexed Artificial Cellular MicroEnvironment (MACME) array for high-throughput manipulation of physical and chemical cues mimicking in vivo cellular microenvironments and to identify the optimal cellular environment for human pluripotent stem cells (hPSCs) with single-cell profiling.

多重の人工細胞の微小環境配列の作製

Cellular microenvironments consist of a variety of cues, such as growth factors, extracellular matrices, and intercellular interactions. These cues are ...

Fabrication of Gradient Nanopattern by Thermal Nanoimprinting Technique and Screening of the Response of Human Endothelial Colony-forming Cells

Nanotopography can be found in various extracellular matrices (ECMs) around the body and is known to have important regulatory actions upon cellular reactions. However, it is difficult to determine the relation between the size of a nanostructure and the responses of cells owing to the lack of proper screening tools. Here, we show the development of reproducible and cost-effective gradient nanopattern plates for the manipulation of cellular responses. Using anodic aluminum oxide (AAO) as a master mold, gradient nanopattern plates with nanopillars of increasing diameter ranges [120-200&#8239;nm (GP 120/200), 200-280&#8239;nm (GP 200/280), and 280-360&#8239;nm (GP 280/360)] were fabricated by a thermal imprinting technique. These gradient nanopattern plates were designed to mimic the various sizes of nanotopography in the ECM and were used to screen the responses of human endothelial colony-forming cells (hECFCs). In this protocol, we describe the step-by-step procedure of fabricating gradient nanopattern plates for cell engineering, techniques of cultivating hECFCs from human peripheral blood, and culturing hECFCs on nanopattern plates.

Here, we present a protocol for the fabrication of gradient nanopattern plates via thermal nanoimprinting and the method of screening responses of human endothelial progenitor cells to the nanostructures. By using the described technology, it is possible to produce a scaffold that can manipulate cell behavior by physical stimuli.

熱ナノイン プリント技術とヒトの内皮細胞コロニー形成細胞の応答のスクリーニングによるグラデーションによるナノパターンの作製

Nanotopography can be found in various extracellular matrices (ECMs) around the body and is known to have important regulatory actions upon cellular ...

Procedure for Fabricating Biofunctional Nanofibers

Electrospinning is an effective processing method for preparing nanofibers decorated with functional groups. Nanofibers decorated with functional groups may be utilized to study material-biomarker interactions i.e. act as biosensors with potential as single molecule detectors. We have developed an effective approach for preparing functional polymers where the functionality has the capacity of specifically binding with a model protein. In our model system, the functional group is 2,4-dinitrophenyl (DNP) and the protein is anti-DNP IgE (Immunoglobulin E). The functional polymer, &alpha;,&omega;-bi[2,4-dinitrophenyl caproic][poly(ethylene oxide)-b-poly(2-methoxystyrene)-b-poly(ethylene oxide)] (CDNP-PEO-P2MS-PEO-CDNP), is prepared by anionic living polymerization. The difunctional initiator utilized in the polymerization was prepared by electron transfer reaction of &alpha;-methylstyrene and potassium (mirror) metal. The 2-methoxystyrene monomer was added first to the initiator, followed by the addition of the second monomer, ethylene oxide, and finally the living polymer was terminated by methanol. The &alpha;,&omega;-dihydroxyl polymer [HO-PEO-P2MS-PEO-OH] was reacted with N-2,4-DNP-&isin;-amino caproic acid, by DCC coupling, resulting in the formation of &alpha;,&omega;-bi[2,4-dinitrophenylcaproic][poly(ethyleneoxide)-b-poly(2-methoxystyrene)-b-poly(ethylene oxide)] (CDNP-PEO-P2MS-PEO-CDNP). The polymers were characterized by FT-IR, 1H NMR and Gel Permeation Chromatography (GPC). The molecular weight distributions of the polymers were narrow (1.1-1.2) and polymers with molecular weights greater than 50,000 was used in this study. The polymers were yellow powders and soluble in tetrahydrofuran. A water soluble CDNP-PEO-P2MS-PEO-CDNP/ DMEG (dimethoxyethylene glycol) complex binds and achieves steady state binding with solution IgE within a few seconds. Higher molecular weight (water insoluble i.e. around 50,000) CDNP-PEO-P2MS-PEO-CDNP polymers, containing 1% single wall carbon nanotubes (SWCNT) were processed into electroactive nanofibers (100 nm to 500 nm in diameter) on silicon substrate. Fluorescence spectroscopy shows that anti-DNP IgE interacts with the nanofibers by binding with the DNP functional groups decorating the fibers. These observations suggest that appropriately functionalized nanofibers hold promise for developing biomarker detection device.

An efficient approach for preparing nanofibers decorated with functional groups capable of specifically interacting with proteins is described. The approach first requires the preparation of a polymer functionalized with the appropriate functional group. The functional polymer is fabricated into nanofibers by electrospinning. The effectiveness of the binding of the nanofibers with a protein is studied by confocal microscopy.

バイオ機能ナノファイバーを製造するための手順

Electrospinning is an effective processing method for preparing nanofibers decorated with functional groups. Nanofibers decorated with functional groups ...

生物学

カーボンナノファイバーアレイを用いた効率的な生体分子および植物への色素送達

Using Vertically Aligned Carbon Nanofiber Arrays on Rigid or Flexible Substrates for Delivery of Biomolecules and Dyes to Plants

The delivery of biomolecules and impermeable dyes to intact plants is a major challenge. Nanomaterials are up-and-coming tools for the delivery of DNA to plants. As exciting as these new tools are, they have yet to be widely applied. Nanomaterials fabricated on rigid substrate (backing) are particularly difficult to successfully apply to curved plant structures. This study describes the process for microfabricating vertically aligned carbon nanofiber arrays and transferring them from a rigid to a flexible substrate. We detail and demonstrate how these fibers (on either rigid or flexible substrates) can be used for transient transformation or dye (e.g., fluorescein) delivery to plants. We show how VACNFs can be transferred from rigid silicon substrate to a flexible SU-8 epoxy substrate to form flexible VACNF arrays. To overcome the hydrophobic nature of SU-8, fibers in the flexible film were coated with a thin silicon oxide layer (2-3 nm). To use these fibers for delivery to curved plant organs, we deposit a 1 &#181;L droplet of dye or DNA solution on the fiber side of VACNF films, wait 10 min, place the films on the plant organ and employ a swab with a rolling motion to drive fibers into plant cells. With this method, we have achieved dye and DNA delivery in plant organs with curved surfaces.

著者スポットライト:カーボンナノファイバーアレイによるイノベーションによるプラント変革の解き放つ 

Here we describe methods for microfabricating vertically aligned carbon nanofibers (VACNFs), transferring VACNFs to flexible substrates, and applying VACNFs on both rigid and flexible substrates to plants for biomolecule and dye delivery.

硬質または軟質基板上に垂直に配向したカーボンナノファイバーアレイを使用して、生体分子および色素を植物に送達

The delivery of biomolecules and impermeable dyes to intact plants is a major challenge. Nanomaterials are up-and-coming tools for the delivery of DNA to ...

マルチスケール構造機能表面の刻印ナノファイバーによる集計

要約

さらに動画を探す

この動画の章

機能材料のNanomoulding、ナノインプリントへ多目的相補パターンレプリケーション方法

マイクロ流体浸透を通じて立体的微細構造ナノ複合材料の製造

表面開始アセンブリの使用ECMタンパク質のナノファイバーおよびナノ構造エンジニア

ブラシのような反応性表面を加工し、アズラクトン官能基化ブロック共重合体の架橋膜

繊維組織における階調の電界紡糸ナノファイバー足場

細胞の挙動のNanotopographical変調のステッチ法を用いたナノパターン基板を拡大

多重の人工細胞の微小環境配列の作製

熱ナノインプリント技術とヒトの内皮細胞コロニー形成細胞の応答のスクリーニングによるグラデーションによるナノパターンの作製

バイオ機能ナノファイバーを製造するための手順

硬質または軟質基板上に垂直に配向したカーボンナノファイバーアレイを使用して、生体分子および色素を植物に送達

マルチ スケール構造機能表面の刻印ナノファイバーによる集計

要約

さらに動画を探す

この動画の章

機能材料のNanomoulding、ナノインプリントへ多目的相補パターンレプリケーション方法

マイクロ流体浸透を通じて立体的微細構造ナノ複合材料の製造

表面開始アセンブリの使用ECMタンパク質のナノファイバーおよびナノ構造エンジニア

ブラシのような反応性表面を加工し、アズラクトン官能基化ブロック共重合体の架橋膜

繊維組織における階調の電界紡糸ナノファイバー足場

細胞の挙動のNanotopographical変調のステッチ法を用いたナノパターン基板を拡大

多重の人工細胞の微小環境配列の作製

熱ナノイン プリント技術とヒトの内皮細胞コロニー形成細胞の応答のスクリーニングによるグラデーションによるナノパターンの作製

バイオ機能ナノファイバーを製造するための手順

硬質または軟質基板上に垂直に配向したカーボンナノファイバーアレイを使用して、生体分子および色素を植物に送達

マルチスケール構造機能表面の刻印ナノファイバーによる集計

熱ナノインプリント技術とヒトの内皮細胞コロニー形成細胞の応答のスクリーニングによるグラデーションによるナノパターンの作製