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Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

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09:50 min

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August 14th, 2019

DOI :

10.3791/59336-v

August 14th, 2019

•

9,240 Views

Zhiping Zhang¹, Shuhang Fan¹, Zhiqiang Chen¹, Yanzhi He¹, Mengfei Huang¹, Li Ding¹, Yanyan Zhang⁵, Lin Chen¹, Xinyue Zhang¹^,²^,³^,⁴

¹College of Bioscience and Biotechnology, Yangzhou University, ²Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, ³Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, ⁴Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ⁵The Testing Center, Yangzhou University

章

文字起こし

要約

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Flavonol Biosynthesis

Flavanone

One pot Bienzymatic Cascade

Recombinant Enzymes

Secondary Metabolite Production

Synthetic Buffer

Flavonoid Stock Solution

TLC Analysis

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