Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade

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9.3K Views

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09:50 min

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August 14th, 2019

DOI :

10.3791/59336-v

August 14th, 2019

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Zhiping Zhang¹, Shuhang Fan¹, Zhiqiang Chen¹, Yanzhi He¹, Mengfei Huang¹, Li Ding¹, Yanyan Zhang⁵, Lin Chen¹, Xinyue Zhang¹^,²^,³^,⁴

¹College of Bioscience and Biotechnology, Yangzhou University, ²Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, ³Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, ⁴Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, ⁵The Testing Center, Yangzhou University

Podsumowanie

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Biosynthesis

Flavonol

Flavanone

One pot Bienzymatic Cascade

Alpha Ketoglutaric Acid

Glycerin

Ferrous Sulfate Heptahydrate

Flavonoid Stock Solution

TLC Analysis

Ethyl Acetate

Organic Phase

Rozdziały w tym wideo

0:04

Title

1:13

Produce a Flavonol from a Flavanone in an in vitro Bienzyme Synthetic System

2:48

Thin Layer Chromatography (TLC) Analysis

5:21

High Performance Liquid Chromatography (HPLC) and Liquid Chromatography/ Mass Spectrometry (LC/MS) Analyses

7:50

Results: Conversion of Flavanone into Flavono

9:16

Conclusion

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