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JoVE Journal

Developmental Biology

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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

4.9K Views

06:53 min

July 10th, 2021

July 10th, 2021

4,860 Views

0:00

Introduction

1:11

Protocol

3:52

Results

5:38

Conclusion

文字起こし

The maintaining of oocyte potential viability after longterm storage represent a tool of great opportunity, as it would improve domestic animal breeding by genetic selection programs, contribute to preserve biodiversity through wildlife species co

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The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

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