<meta charset="utf8"></head><body>UPLC-TOF質量分析による Gynura bicolor の化学組成の同定

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<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>chloroform</td>
			<td>Sinopharm Chemical ReagentCo., Ltd</td>
			<td>CAS 67-66-3</td>
			<td></td>
		</tr>
		<tr>
			<td>ethanol</td>
			<td>ChuandongChemical</td>
			<td>CAS 64-17-5</td>
			<td></td>
		</tr>
		<tr>
			<td>ethyl acetate</td>
			<td>ChuandongChemical</td>
			<td>CAS 141-78-6</td>
			<td></td>
		</tr>
		<tr>
			<td>liquid chromatograph</td>
			<td>Waters</td>
			<td>ACQUITY Class 1 plus</td>
			<td></td>
		</tr>
		<tr>
			<td>MassLynx</td>
			<td>Waters</td>
			<td>V4.2</td>
			<td>MS control software</td>
		</tr>
		<tr>
			<td>n-butyl alcohol</td>
			<td>ChuandongChemical</td>
			<td>CAS 71-36-3</td>
			<td></td>
		</tr>
		<tr>
			<td>petroleum ether</td>
			<td>ChuandongChemical</td>
			<td>CAS 8032-32-4</td>
			<td></td>
		</tr>
		<tr>
			<td>Quadrupole time-of-flight mass spectrometry</td>
			<td>Waters</td>
			<td>SYNAPT XS</td>
			<td></td>
		</tr>
		<tr>
			<td>UNIFI</td>
			<td>Waters</td>
			<td></td>
			<td>Data analysis software</td>
		</tr>
	</tbody>
</table>

high-resolution tandem mass spectrometry for studying chemical constituents of gynura bicolor dc

Traditional Chinese medicine (TCM) has been clinically practiced in China for thousands of years, and it plays a vital role in maintaining the health of Chinese people1. The composition of TCM is diverse and complex, and TCM has been widely reported in many qualitative studies focusing on the chemical composition2. The chemical components in TCM can be roughly divided into the following categories such as alkaloids, organic acids, phenylpropanoids, coumarins, lignans, quinones, flavonoids, terpenoids, triterpenoid saponins, steroid saponins, cardiac glycosides, and tannins3. Given the large numbers of unknown components and indistinguishable isomers in TCM, the separation and analysis of the desired chemical components are important subjects for the fundamental research of TCM4.
Ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) has been applied to analyze substances in traditional Chinese medicine (TCM), which can be separated by ultra-high-performance liquid chromatography5,6. The high resolution of MS can provide extensive ion information, which is used for database analysis with error less than 5 ppm7. After turning on the collision energy, the secondary MS mode can obtain secondary fragment ions, whose intensity and number are affected by the magnitude of energy8. 
Gynura bicolor DC. (BFH), a perennial stemless herb widely used for medicine and food (Figure 1A), is a rare and endangered plant unique to China9. BFH has abundant anthocyanins, polyphenols, flavonoids, and strong antioxidant capacity10. BFH has medicinal effects including clearing heat, cooling blood, moistening the lung, relieving cough, dispersing stasis, relieving swelling, relieving summer heat, and eliminating heat. Few studies have focused on the chemical composition of BFH11. Polyphenols and flavonoids contain numerous isomers, which make the identification of the complex compounds in BFH difficult. A universal method for the identification of chemical components must be developed, which can be applied to all kinds of TCM. This study aimed to report a systematic protocol for studying chemical constituents of BFH based on solvent extraction and integrated data via UPLC-Q-TOF-MS.

<meta charset="utf8"></head><body>高分解能タンデム質量分析のための GynuraバイカラーDC サンプルの調製

Gynura bicolor DCの化学成分を研究するための高分解能タンデム質量分析

Begin by sample pretreatment. Wash the whole herb of BFH in pure water until there are no visible deposits and impurities. Place the clean BFH in a dish and then place it in the oven at 50 degrees Celsius for 24 hours.Crush the dried BFH in a high-speed multifunctional crusher. Transfer the coarse powder into a 50-mesh sieve. Collect the fine powder in an airtight bag and store it in a drying tower.Then accurately weigh six BFH samples of 0.25 grams each and place them in six conical flasks. Add 30 milliliters of organic solvent to each conical flask. Transfer the mixture to an ultrasound bath sonicator for 30 minutes at 25 degrees Celsius.Next, centrifuge the sample at 14, 000g for five minutes. Using an injection syringe and the 0.22-micrometer organic microporous membrane filter, filter all the supernatants into two-milliliter sample bottles.

Read this Scientific Article Protocol about High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC at JoVE.com

The separation and analysis of the desired chemical components are important subjects for the fundamental research of traditional Chinese medicine (TCM). ...

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High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

283 ビュー.  Chongqing Hospital of Traditional Chinese Medicine. サンプルの前処理から始めます。目に見える堆積物や不純物がなくなるまで、BFHのハーブ全体を純水で洗います。きれいなBFHを皿に入れ、摂氏50度のオーブンに24時間置きます。乾燥させたBFHを高速多機能破砕機で粉砕します。粗い粉末を50メッシュのふるいに移します。微粉末を密閉袋に集め、乾燥塔に保管します。次に、それぞれ0....