JoVE Journal

Chemistry

A subscription to JoVE is required to view this content.

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

Transcript

Begin by sample pretreatment. Wash the whole herb of BFH in pure water until there are no visible deposits and impurities. Place the clean BFH in a dish and then place it in the oven at 50 degrees Celsius for 24 hours.

Crush the dried BFH in a high-speed multifunctional crusher. Transfer the coarse powder into a 50-mesh sieve. Collect the fine powder in an airtight bag and store it in a drying tower.

Then accurately weigh six BFH samples of 0.25 grams each and place them in six conical flasks. Add 30 milliliters of organic solvent to each conical flask. Transfer the mixture to an ultrasound bath sonicator for 30 minutes at 25 degrees Celsius.

Next, centrifuge the sample at 14, 000g for five minutes. Using an injection syringe and the 0.22-micrometer organic microporous membrane filter, filter all the supernatants into two-milliliter sample bottles.

We describe a general protocol that integrates high-resolution mass spectrometry analysis and molecular docking in traditional Chinese medicine research.

Chapters in this video

0:00

Introduction

1:14

Calibration and Data Acquisition on High‐Resolution Tandem Mass Spectrometer for Chemical Composition Analysis

4:16

Processing and Analysis of Mass Spectrometry Data for Chemical Composition Analysis of Gynura bicolor DC

Related Videos

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More