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High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC

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07:16 min

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February 2nd, 2024

DOI :

10.3791/66612-v

February 2nd, 2024

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Yujuan Xiao*¹, Xiaofeng Han*¹, Xi Chen¹, Chunyan Zhou², Yanxi Li¹, Deming Liu¹

¹Chongqing Clinical Research Center for Dermatology, Chongqing Key Laboratory of Integrative Dermatology Research, Key Laboratory of External Therapies of Traditional Chinese Medicine in Eczema, Department of Dermatology, Chongqing Hospital of Traditional Chinese Medicine, ²General Surgery, Chongqing Hospital of Traditional Chinese Medicine

Transcript

Begin by sample pretreatment. Wash the whole herb of BFH in pure water until there are no visible deposits and impurities. Place the clean BFH in a dish and then place it in the oven at 50 degrees Celsius for 24 hours.

Crush the dried BFH in a high-speed multifunctional crusher. Transfer the coarse powder into a 50-mesh sieve. Collect the fine powder in an airtight bag and store it in a drying tower.

Then accurately weigh six BFH samples of 0.25 grams each and place them in six conical flasks. Add 30 milliliters of organic solvent to each conical flask. Transfer the mixture to an ultrasound bath sonicator for 30 minutes at 25 degrees Celsius.

Next, centrifuge the sample at 14, 000g for five minutes. Using an injection syringe and the 0.22-micrometer organic microporous membrane filter, filter all the supernatants into two-milliliter sample bottles.

Summary

We describe a general protocol that integrates high-resolution mass spectrometry analysis and molecular docking in traditional Chinese medicine research.

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Tandem Mass Spectrometry

Gynura Bicolor DC

Sample Pretreatment

Organic Solvent Extraction

UPLC Q TOF MS MS

Chemical Constituents

Traditional Chinese Medicine

Polyphenols

Flavonoids

Data Processing

Fragmentation Pattern

Medicinal Effects

Sample Analysis

Calibration Methods

Chapters in this video

0:00

Introduction

1:14

Calibration and Data Acquisition on High‐Resolution Tandem Mass Spectrometer for Chemical Composition Analysis