Wei-Jun Qian

Phosphoprotein isotope-coded solid-phase tag approach for enrichment and quantitative analysis of phosphopeptides from complex mixtures.

High-throughput comparative proteome analysis using a quantitative cysteinyl-peptide enrichment technology.

Simultaneous monitoring of Zn2+ secretion and intracellular Ca2+ from islets and islet cells by fluorescence microscopy.

Comparative proteome analyses of human plasma following in vivo lipopolysaccharide administration using multidimensional separations coupled with tandem mass spectrometry.

Targeted comparative proteomics by liquid chromatography-tandem Fourier ion cyclotron resonance mass spectrometry.

Probability-based evaluation of peptide and protein identifications from tandem mass spectrometry and SEQUEST analysis: the human proteome.

Improved proteome coverage by using high efficiency cysteinyl peptide enrichment: the human mammary epithelial cell proteome.

Quantitative proteome analysis of human plasma following in vivo lipopolysaccharide administration using 16O/18O labeling and the accurate mass and time tag approach.

High-throughput proteomics using Fourier transform ion cyclotron resonance mass spectrometry.

Utilizing human blood plasma for proteomic biomarker discovery.

Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.

Development and evaluation of a micro- and nanoscale proteomic sample preparation method.

Advances in proteomics data analysis and display using an accurate mass and time tag approach.

Normalization approaches for removing systematic biases associated with mass spectrometry and label-free proteomics.

Characterization of the mouse brain proteome using global proteomic analysis complemented with cysteinyl-peptide enrichment.

High dynamic range characterization of the trauma patient plasma proteome.

Endogenously nitrated proteins in mouse brain: links to neurodegenerative disease.

Evaluation of multiprotein immunoaffinity subtraction for plasma proteomics and candidate biomarker discovery using mass spectrometry.

Advances and challenges in liquid chromatography-mass spectrometry-based proteomics profiling for clinical applications.

Characterization of the human pancreatic islet proteome by two-dimensional LC/MS/MS.

Spatial mapping of protein abundances in the mouse brain by voxelation integrated with high-throughput liquid chromatography-mass spectrometry.

Quantitative proteomic approaches for studying phosphotyrosine signaling.

The use of a quantitative cysteinyl-peptide enrichment technology for high-throughput quantitative proteomics.

Quantitative analysis of human immunodeficiency virus type 1-infected CD4+ cell proteome: dysregulated cell cycle progression and nuclear transport coincide with robust virus production.

Profiling signaling polarity in chemotactic cells.

A method for selective enrichment and analysis of nitrotyrosine-containing peptides in complex proteome samples.

A genome-scale map of expression for a mouse brain section obtained using voxelation.

Accurate mass measurements in proteomics.

Capillary-based multi nanoelectrospray emitters: improvements in ion transmission efficiency and implementation with capillary reversed-phase LC-ESI-MS.

Application of proteomics in the discovery of candidate protein biomarkers in a diabetes autoantibody standardization program sample subset.

Elimination of systematic mass measurement errors in liquid chromatography-mass spectrometry based proteomics using regression models and a priori partial knowledge of the sample content.

Mitochondrial dysfunction, oxidative stress, and apoptosis revealed by proteomic and transcriptomic analyses of the striata in two mouse models of Parkinson's disease.

Proteome-wide identification of proteins and their modifications with decreased ambiguities and improved false discovery rates using unique sequence tags.

Quantitative proteome mapping of nitrotyrosines.

DAnTE: a statistical tool for quantitative analysis of -omics data.

Characterization of the mouse pancreatic islet proteome and comparative analysis with other mouse tissues.

Enhanced detection of low abundance human plasma proteins using a tandem IgY12-SuperMix immunoaffinity separation strategy.

Quantitative phosphoproteome analysis of lysophosphatidic acid induced chemotaxis applying dual-step (18)O labeling coupled with immobilized metal-ion affinity chromatography.

Large-scale multiplexed quantitative discovery proteomics enabled by the use of an (18)O-labeled "universal" reference sample.

A simple procedure for effective quenching of trypsin activity and prevention of 18O-labeling back-exchange.

Dual spatial maps of transcript and protein abundance in the mouse brain.

An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells.

A statistical framework for protein quantitation in bottom-up MS-based proteomics.

Mapping protein abundance patterns in the brain using voxelation combined with liquid chromatography and mass spectrometry.

Mouse-specific tandem IgY7-SuperMix immunoaffinity separations for improved LC-MS/MS coverage of the plasma proteome.

N-terminal enrichment: developing a protocol to detect specific proteolytic fragments.

Endogenous 3,4-dihydroxyphenylalanine and dopaquinone modifications on protein tyrosine: links to mitochondrially derived oxidative stress via hydroxyl radical.

Region-specific protein abundance changes in the brain of MPTP-induced Parkinson's disease mouse model.

Quantitative analysis of cell surface membrane proteins using membrane-impermeable chemical probe coupled with 18O labeling.

Enhanced sensitivity for selected reaction monitoring mass spectrometry-based targeted proteomics using a dual stage electrodynamic ion funnel interface.

Sensitive immunoassays of nitrated fibrinogen in human biofluids.

Shotgun proteomics identifies proteins specific for acute renal transplant rejection.

CD90/THY1 is overexpressed in prostate cancer-associated fibroblasts and could serve as a cancer biomarker.

Plasma proteome response to severe burn injury revealed by 18O-labeled "universal" reference-based quantitative proteomics.

Clinical microfluidics for neutrophil genomics and proteomics.

Improved LC-MS/MS spectral counting statistics by recovering low-scoring spectra matched to confidently identified peptide sequences.

Trypsin-catalyzed oxygen-18 labeling for quantitative proteomics.

Liquid chromatography-mass spectrometry-based quantitative proteomics.

Identification of c-type heme-containing peptides using nonactivated immobilized metal affinity chromatography resin enrichment and higher-energy collisional dissociation.

Unraveling pancreatic islet biology by quantitative proteomics.

IgY14 and SuperMix immunoaffinity separations coupled with liquid chromatography-mass spectrometry for human plasma proteomics biomarker discovery.

Quantitative proteomics analysis of adsorbed plasma proteins classifies nanoparticles with different surface properties and size.

18O-labeled proteome reference as global internal standards for targeted quantification by selected reaction monitoring-mass spectrometry.

Mapping N-linked glycosylation sites in the secretome and whole cells of Aspergillus niger using hydrazide chemistry and mass spectrometry.

Simple sodium dodecyl sulfate-assisted sample preparation method for LC-MS-based proteomics applications.

Identification of phosphorylated butyrylcholinesterase in human plasma using immunoaffinity purification and mass spectrometry.

Advancing the sensitivity of selected reaction monitoring-based targeted quantitative proteomics.

Analysis of serum total and free PSA using immunoaffinity depletion coupled to SRM: correlation with clinical immunoassay tests.

Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum.

In-source fragmentation and the sources of partially tryptic peptides in shotgun proteomics.

Quantitative Site-specific Reactivity Profiling of S-nitrosylation in Mouse Skeletal Muscle Using Cysteinyl Peptide Enrichment Coupled with Mass Spectrometry.

Liver-Derived Systemic Factors Drive β Cell Hyperplasia in Insulin-Resistant States.

Synthesis and application of an environmentally insensitive Cy3-based arsenical fluorescent probe to identify adaptive microbial responses involving proximal dithiol oxidation.

Determination of burn patient outcome by large-scale quantitative discovery proteomics.

Trauma-associated human neutrophil alterations revealed by comparative proteomics profiling.

Evaluation of selected binding domains for the analysis of ubiquitinated proteomes.

Targeted quantification of low ng/mL level proteins in human serum without immunoaffinity depletion.

Resin-assisted enrichment of N-terminal peptides for characterizing proteolytic processing.

Long-gradient separations coupled with selected reaction monitoring for highly sensitive, large scale targeted protein quantification in a single analysis.

Impact of alg3 gene deletion on growth, development, pigment production, protein secretion, and functions of recombinant Trichoderma reesei cellobiohydrolases in Aspergillus niger.

A highly sensitive targeted mass spectrometric assay for quantification of AGR2 protein in human urine and serum.

Proteomic identification and quantification of S-glutathionylation in mouse macrophages using resin-assisted enrichment and isobaric labeling.

The identification of novel potential injury mechanisms and candidate biomarkers in renal allograft rejection by quantitative proteomics.

Resin-assisted enrichment of thiols as a general strategy for proteomic profiling of cysteine-based reversible modifications.

Optimization for peptide sample preparation for urine peptidomics.

High and low doses of ionizing radiation induce different secretome profiles in a human skin model.

Detecting differential protein expression in large-scale population proteomics.

Proteome-wide light/dark modulation of thiol oxidation in cyanobacteria revealed by quantitative site-specific redox proteomics.

A method to determine lysine acetylation stoichiometries.

Expediting SRM assay development for large-scale targeted proteomics experiments.

Antibody-independent targeted quantification of TMPRSS2-ERG fusion protein products in prostate cancer.

Brain insulin lowers circulating BCAA levels by inducing hepatic BCAA catabolism.

Proteomic approaches for site-specific O-GlcNAcylation analysis.

Sensitive targeted quantification of ERK phosphorylation dynamics and stoichiometry in human cells without affinity enrichment.

Perturbations in the urinary exosome in transplant rejection.

Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods.

Compensatory Islet Response to Insulin Resistance Revealed by Quantitative Proteomics.

Preserved DNA Damage Checkpoint Pathway Protects against Complications in Long-Standing Type 1 Diabetes.

The clinical impact of recent advances in LC-MS for cancer biomarker discovery and verification.

The Measurement of Reversible Redox Dependent Post-translational Modifications and Their Regulation of Mitochondrial and Skeletal Muscle Function.

Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function during Nanoparticle-Induced Oxidative Stress.

SerpinB1 Promotes Pancreatic β Cell Proliferation.

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry-Based Assays.

SNaPP: Simplified Nanoproteomics Platform for Reproducible Global Proteomic Analysis of Nanogram Protein Quantities.

Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays.

Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids.

9-Fluorenylmethyl (Fm) Disulfides: Biomimetic Precursors for Persulfides.

The current state of the art of quantitative phosphoproteomics and its applications to diabetes research.

Mining the human urine proteome for monitoring renal transplant injury.

Advances in targeted proteomics and applications to biomedical research.

Conservation of protein abundance patterns reveals the regulatory architecture of the EGFR-MAPK pathway.

An efficient method for native protein purification in the selected range from prostate cancer tissue digests.

Biodegradation of alkaline lignin by L1.

Quantitative proteomic characterization of redox-dependent post-translational modifications on protein cysteines.

Targeted quantification of functional enzyme dynamics in environmental samples for microbially mediated biogeochemical processes.

Quantitative proteomics identifies altered O-GlcNAcylation of structural, synaptic and memory-associated proteins in Alzheimer's disease.

Targeted proteomic assays for quantitation of proteins identified by proteogenomic analysis of ovarian cancer.

Deep-Dive Targeted Quantification for Ultrasensitive Analysis of Proteins in Nondepleted Human Blood Plasma/Serum and Tissues.

Advances in microscale separations towards nanoproteomics applications.

Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics.

Functional Enzyme-Based Approach for Linking Microbial Community Functions with Biogeochemical Process Kinetics.

-Nitrosylation inhibits the kinase activity of tomato phosphoinositide-dependent kinase 1 (PDK1).

LC-SRM-Based Targeted Quantification of Urinary Protein Biomarkers.

Multiplexed targeted mass spectrometry assays for prostate cancer-associated urinary proteins.

Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells.

Subnanogram proteomics: impact of LC column selection, MS instrumentation and data analysis strategy on proteome coverage for trace samples.

Targeted Quantification of Phosphorylation Dynamics in the Context of EGFR-MAPK Pathway.

Optimization for Peptide Sample Preparation for Urine Peptidomics.

Urinary Virome Perturbations in Kidney Transplantation.

Fatiguing contractions increase protein S-glutathionylation occupancy in mouse skeletal muscle.

Spatially Resolved Proteome Mapping of Laser Capture Microdissected Tissue with Automated Sample Transfer to Nanodroplets.

Mass spectrometry-based proteomics for system-level characterization of biological responses to engineered nanomaterials.

Serum biomarkers for diagnosis and prediction of type 1 diabetes.

Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells.

Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells.

Insulin receptor-mediated signaling regulates pluripotency markers and lineage differentiation.

Nanoproteomics comes of age.

Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes.

Carrier-Assisted Single-Tube Processing Approach for Targeted Proteomics Analysis of Low Numbers of Mammalian Cells.

Improving mitochondrial function with SS-31 reverses age-related redox stress and improves exercise tolerance in aged mice.

Genomics and biochemistry investigation on the metabolic pathway of milled wood and alkali lignin-derived aromatic metabolites of SP-35.

Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells.

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of strains.

Architecture and subunit arrangement of native AMPA receptors elucidated by cryo-EM.

Response to Comment on Sims et al. Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes. Diabetes Care 2019;42:258-264.

Stable Acinar Progenitor Cell Model Identifies Treacle-Dependent Radioresistance.

The role of proteomics in assessing beta-cell dysfunction and death in type 1 diabetes.

Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples.

Increased β-cell proliferation before immune cell invasion prevents progression of type 1 diabetes.

High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform.

Comprehensive Proteomics Analysis of Stressed Human Islets Identifies GDF15 as a Target for Type 1 Diabetes Intervention.

A proteome-wide assessment of the oxidative stress paradigm for metal and metal-oxide nanomaterials in human macrophages.

Evaluation of the cytotoxic and cellular proteome impacts of food-grade TiO (E171) using simulated gastrointestinal digestions and a tri-culture small intestinal epithelial model.

Accurate Identification of Deamidation and Citrullination from Global Shotgun Proteomics Data Using a Dual-Search Delta Score Strategy.

Development of Multiplexed Immuno-N-Terminomics to Reveal the Landscape of Proteolytic Processing in Early Embryogenesis of .

Block Design with Common Reference Samples Enables Robust Large-Scale Label-Free Quantitative Proteome Profiling.

Proteomic Tissue-Based Classifier for Early Prediction of Prostate Cancer Progression.

Late-life restoration of mitochondrial function reverses cardiac dysfunction in old mice.

Stochiometric quantification of the thiol redox proteome of macrophages reveals subcellular compartmentalization and susceptibility to oxidative perturbations.

Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.

Protein thiol oxidation in the rat lung following e-cigarette exposure.

Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.

Characterization of cellular oxidative stress response by stoichiometric redox proteomics.

Enhancement of polyhydroxyalkanoate production by co-feeding lignin derivatives with glycerol in Pseudomonas putida KT2440.

The Identification of Novel Potential Injury Mechanisms and Candidate Biomarkers in Renal Allograft Rejection by Quantitative Proteomics.

Corrigendum: Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney.

Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics.

Defective insulin receptor signaling in hPSCs skews pluripotency and negatively perturbs neural differentiation.

Stoichiometric Thiol Redox Proteomics for Quantifying Cellular Responses to Perturbations.

Enhancing Top-Down Proteomics of Brain Tissue with FAIMS.

Proteomic Profiling of the Substantia Nigra to Identify Determinants of Lewy Body Pathology and Dopaminergic Neuronal Loss.

A Comprehensive Urine Proteome Database Generated From Patients With Various Renal Conditions and Prostate Cancer.

Altered β-Cell Prohormone Processing and Secretion in Type 1 Diabetes.

Repetitive diacetyl vapor exposure promotes ubiquitin proteasome stress and precedes bronchiolitis obliterans pathology.

Meta-analysis of peptides to detect protein significance.

Assessment of TMT Labeling Efficiency in Large-Scale Quantitative Proteomics: The Critical Effect of Sample pH.