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University of Montpellier

3 ARTICLES PUBLISHED IN JoVE

Behavior

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks

Simone Dalla Bella^1,2,3,4, Jakub Sowiński³

¹Movement to Health Laboratory (EuroMov), University of Montpellier, ²Institut Universitaire de France, ³Department of Cognitive Psychology, University of Finance and Management in Warsaw, ⁴International Laboratory for Brain, Music, and Sound Research (BRAMS)

Behavioral tasks that allow for the assessment of perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) are presented. Synchronization of finger tapping to the beat of an auditory stimuli and detecting rhythmic irregularities provides a means of uncovering rhythm disorders.

Neuroscience

Optical Coherence Tomography: Imaging Mouse Retinal Ganglion Cells In Vivo

Jolanta Jagodzinska^*1, Emmanuelle Sarzi^*1, Mélanie Cavalier¹, Marie Seveno¹, Volker Baecker², Christian Hamel^1,3,4, Marie Péquignot¹, Cecile Delettre¹

¹INSERM U1051, Institut of Neurosciences of Montpellier, ²CNRS UMS3426, BioCampus Montepellier, ³University of Montpellier, ⁴CHRU Montpellier, Centre of Reference for Genetic Sensory Diseases, CHU Gui de Chauliac Hospital

This manuscript describes a protocol for the in vivo imaging of the mouse retina with high-resolution spectral domain optical coherence tomography (SD-OCT). It focuses on retinal ganglion cells (RGC) in the peripapillary region, with several scanning and quantifying approaches described.

Bioengineering

Cell-Free Protein Synthesis from Exonuclease-Deficient Cellular Extracts Utilizing Linear DNA Templates

Mahnaz Sabeti Azad^*1, Angelo Cardoso Batista^*1, Jean-Loup Faulon¹, Chase L. Beisel^2,3, Jerome Bonnet⁴, Manish Kushwaha¹

¹INRAe, AgroParisTech, Micalis Institute, Université Paris-Saclay, ²Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz-Centre for Infection Research (HZI), ³Medical Faculty, University of Würzburg, ⁴Centre de Biochimie Structurale, INSERM U1054, CNRS UMR 5048, University of Montpellier

Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 (ΔrecBCD and ΔrecB). This is a fast, easy, and direct approach for expression in cell-free protein synthesis systems using linear DNA templates.