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Mahidol University

7 ARTICLES PUBLISHED IN JoVE

Medicine

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Panithi Sukho^1,2,3, Geesien S.A. Boersema⁴, Nicole Kops⁵, Johan F. Lange⁴, Jolle Kirpensteijn^1,6, Jan Willem Hesselink¹, Yvonne M. Bastiaansen-Jenniskens⁵, Femke Verseijden^1,5

¹Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, ²Department of Otorhinolaryngology, Erasmus MC University Medical Center, ³Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, ⁴Department of Surgery, Erasmus MC University Medical Center, ⁵Department of Orthopaedics, Erasmus MC University Medical Center, ⁶Hill's Pet Nutrition Inc.

The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

Medicine

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Sirada Srihirun^1,2, Alan N. Schechter², Barbora Piknova²

¹Department of Pharmacology, Faculty of Dentistry, Mahidol University, ²Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

Biology

Isolation of Human Neutrophils from Whole Blood and Buffy Coats

Alan Y. Hsu¹, Zhicheng Peng¹, Hongbo Luo¹, Fabien Loison^1,2

¹Department of Pathology, Harvard Medical School, Department of Lab Medicine, Children’s Hospital Boston, and Dana-Farber/Harvard Cancer Center, ²Department of Microbiology, Faculty of Science, Mahidol University

This protocol details a method for the isolation of neutrophils from whole blood, buffy coats, or leukapheresis membranes, achieving good yield, high purity, and minimal cell activation. We utilize gradient purification, red blood cell (RBC) sedimentation, and RBC lysis to obtain a high-quality/purity neutrophil preparation.

Biology

Detection of Plasmodium Sporozoites in Anopheles Mosquitoes using an Enzyme-linked Immunosorbent Assay

Chalermpon Kumpitak¹, Wang Nguitragool², Liwang Cui³, Jetsumon Sattabongkot¹, Sirasate Bantuchai⁴

¹Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University, ²Department of Molecular Tropical Medicine & Genetics, Faculty of Tropical Medicine, Mahidol University, ³Department of Internal Medicine, Morsani College of Medicine, University of South Florida, ⁴Mahidol Vivax Research Unit, Faculty of Tropical Medicine, Mahidol University

This protocol describes a sandwich enzyme-linked immunosorbent assay to detect salivary gland sporozoites in mosquitoes. Using easily available monoclonal antibodies, the method enables cost-effective, high-throughput detection of mosquitoes carrying Plasmodium falciparum or Plasmodium vivax. The method is suitable for malaria transmission research, including vector surveys.

Biochemistry

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Khanit Sa-ngiamsuntorn¹, Piyanoot Thongsri², Yongyut Pewkliang², Suparerk Borwornpinyo^3,4, Adisak Wongkajornsilp⁵

¹Department of Biochemistry, Faculty of Pharmacy, Mahidol University, ²Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, ³Excellent Center for Drug Discovery, Faculty of Science, Mahidol University, ⁴Department of Biotechnology, Faculty of Science, Mahidol University, ⁵Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University

We present a protocol to screen anti-hepatitis B virus (HBV) compounds targeting pre- and post-viral entry lifecycle stages, using isothermal titration calorimetry to measure binding affinity (K_D) with host sodium taurocholate cotransporting polypeptide. Antiviral efficacy was determined through the suppression of viral lifecycle markers (cccDNA formation, transcription, and viral assembly).

A Video Repository for Innovative Methods of Dietary Assessment and Analysis

Sabri Bromage^1,2, Teresa T. Fung³, Sharon I. Kirkpatrick⁴, Walter C. Willett²

¹Institute of Nutrition, Mahidol University, ²Department of Nutrition, Harvard T. H. Chan School of Public Health, ³Department of Nutrition, Simmons University, ⁴School of Public Health Sciences, University of Waterloo

A Video Repository for Innovative Methods of Dietary Assessment and Analysis

Behavior

Temporomandibular Joint Pain Measurement by Bite Force and Von Frey Filament Assays in Mice

Supawadee Jariyasakulroj^1,2, Qing Chang¹, Pao-Fen Ko¹, Yang Shu¹, Ziying Lin¹, Jingyi Chen¹, Jian-Fu Chen¹

¹Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, ²Department of Masticatory Science, Faculty of Dentistry, Mahidol University

This protocol describes how measuring bite force and head withdrawal threshold can capture pain behavior in Complete Freund's Adjuvant (CFA)-induced inflammatory temporomandibular joint (TMJ) pain.