ELISA technique is really useful for detecting malaria parasite in the mosquito vector with high sensitivity and specificity, and able to process lot of sample at the same time. This technique allow researchers to keep mosquito samples until they
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This protocol describes a sandwich enzyme-linked immunosorbent assay to detect salivary gland sporozoites in mosquitoes. Using easily available monoclonal antibodies, the method enables cost-effective, high-throughput detection of mosquitoes carrying Plasmodium falciparum or Plasmodium vivax. The method is suitable for malaria transmission research, including vector surveys.