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H. Lee Moffitt Cancer Center and Research Institute

2 ARTICLES PUBLISHED IN JoVE

Medicine

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Ariosto Silva¹, Timothy Jacobson¹, Mark Meads¹, Allison Distler¹, Kenneth Shain¹

¹Department of Cancer Imaging and Metabolism, H. Lee Moffitt Cancer Center and Research Institute

We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.

Medicine

Cardiac Magnetic Resonance for the Evaluation of Suspected Cardiac Thrombus: Conventional and Emerging Techniques

Elizabeth M. Johnson¹, Kenneth L. Gage², Sebastian Feuerlein², Daniel Jeong²

¹Department of Radiology, University of South Florida, ²Department of Diagnostic Imaging, H. Lee Moffitt Cancer Center and Research Institute

The goal of this article is to describe how cardiac magnetic resonance can be used for the evaluation and diagnosis of a suspected cardiac thrombus. The method presented will describe data acquisition as well as the pre-procedure and post-procedure protocol.