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Hosei University

2 ARTICLES PUBLISHED IN JoVE

Biology

Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Noriko Ueki¹, Atsuko Isu², Ken‒ichi Wakabayashi^2,3

¹Science Research Center, Hosei University, ²Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, ³School of Life Science and Technology, Tokyo Institute of Technology

The in vitro reactivation of motile cells is a crucial experiment in understanding the mechanisms of cell motility. The protocol describes reactivating the demembranated cell models of Chlamydomonas reinhardtii, a model organism to study cilia/flagella.

Biology

Observation of Photobehavior in Chlamydomonas reinhardtii

Noriko Ueki^*1, Atsuko Isu^*2, Ayaka Kyuji^*2,3, Yuma Asahina^*2,3, Satoaki So^*2,3, Rina Takahashi^*2,3, Toru Hisabori^2,3, Ken‒ichi Wakabayashi^2,3

Most swimming photoautotrophic organisms show photo-induced behavioral changes (photobehavior). The present protocol observes the said photobehavior in the model organism Chlamydomonas reinhardtii.