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shandong agricultural university

4 ARTICLES PUBLISHED IN JoVE

Biology

Observation and Quantification of Mating Behavior in the Pinewood Nematode, Bursaphelenchus xylophilus

Najie Zhu^*1, Liqun Bai^*1, Stefan Schütz², Baojun Liu³, Zhenyu Liu³, Xingyao Zhang⁴, Hongshi Yu¹, Jiafu Hu^1,5

¹Department of Forest Protection, Zhejiang Agricultural & Forestry University, ²Institute of Forest Zoology and Forest Conservation, Georg-August University Göttingen, ³College of Plant Protection, Shandong Agricultural University, ⁴Institute of Forest Protection, Chinese Academy of Forestry, ⁵Collaborative Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University

A protocol for investigating the mating behavior of the pinewood nematode, Bursaphelenchus xylophilus, is presented. Behavioral features of B. xylophilus are described in the mating process.

Neuroscience

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Zhenyu Liu¹, Katelynne B. Donnelly¹, Kara G. Pratt¹

¹Department of Zoology and Physiology and Program in Neuroscience, University of Wyoming

In this paper, we discuss three brain preparations used for whole cell patch clamp recording to study the retinotectal circuit of Xenopus laevis tadpoles. Each preparation, with its own specific advantages, contributes to the experimental tractability of the Xenopus tadpole as a model to study neural circuit function.

Biochemistry

Evaluating the Effect of Pesticides on the Larvae of the Solitary Bees

Yingying Song¹, Ruiping Li^1,2, Lili Li¹, Fang Ouyang³, Xingyuan Men¹

¹Institute of Plant Protection, Shandong Academy of Agricultural Sciences, ²College of Plant Protection, Shandong Agricultural University, ³State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences

The present protocol explains a method to feed pesticide-contaminated provisions to the larvae of the solitary bees, Osmia excavata. The procedure examines the ecotoxicity of the pesticide to the larvae of the solitary bees.

Environment

Determination of the Absorption, Translocation, and Distribution of Imidacloprid in Wheat

Jinhua Wang^*1, Chao Cheng^*1,2, Changyu Zhao¹, Lanjun Wang¹

¹Key Laboratory of Agricultural Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, ²College of Water Conservancy and Civil Engineering, Shandong Agricultural University

Presented here is a protocol for the determination of the absorption, translocation, and distribution of imidacloprid in wheat under hydroponic conditions using liquid chromatography-tandem mass spectrometry (LC-MS-MS). The results showed that imidacloprid can be absorbed by wheat, and imidacloprid was detected in both the wheat roots and leaves.