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Royal Infirmary of Edinburgh

3 ARTICLES PUBLISHED IN JoVE

Medicine

In situ Transverse Rectus Abdominis Myocutaneous Flap: A Rat Model of Myocutaneous Ischemia Reperfusion Injury

Marie-Claire Edmunds¹, Stephen Wigmore¹, David Kluth²

¹Department of Surgery, Royal Infirmary of Edinburgh, ²Department of Nephrology, Royal Infirmary of Edinburgh

Free tissue transfer is widely employed in reconstructive surgery to restore form and function following oncological resection and trauma. Preconditioning this tissue prior to surgery may improve outcome. This article describes an in situ transverse rectus abdominis myocutaneous flap (TRAM) in rats as a means for testing preconditioning strategies.

Medicine

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Emily E. Hesketh¹, Alicja Czopek¹, Michael Clay¹, Gary Borthwick¹, David Ferenbach¹, David Kluth¹, Jeremy Hughes¹

¹MRC Centre for Inflammation Research, University of Edinburgh

The mouse model of renal ischaemia reperfusion injury described here comprises of a right nephrectomy that provides control tissue and clamping of the left renal pedicle to induce ischaemia that results in acute kidney injury. This model uses a midline laparotomy approach with all steps performed via one incision.

Bioengineering

High-throughput Identification of Bacteria Repellent Polymers for Medical Devices

Seshasailam Venkateswaran^*1, Peter J. Gwynne^*2, Mei Wu¹, Ailsa Hardman², Annamaria Lilienkampf¹, Salvatore Pernagallo¹, Garry Blakely², David G. Swann³, Mark Bradley¹, Maurice P. Gallagher²

¹School of Chemistry, EaStCHEM, University of Edinburgh, ²School of Biological Sciences, University of Edinburgh, ³Critical Care, NHS Lothian, Royal Infirmary of Edinburgh

A high-throughput microarray method for the identification of polymers which reduce bacterial surface binding on medical devices is described.