로그인

University Witten/Herdecke

7 ARTICLES PUBLISHED IN JoVE

Biology

Monitoring Acupuncture Effects on Human Brain by fMRI

Kathleen K. S. Hui¹, Vitaly Napadow¹, Jing Liu¹, Ming Li¹, Ovidiu Marina^1,2, Erika E. Nixon¹, Joshua D. Claunch¹, Lauren LaCount¹, Tara Sporko¹, Kenneth K. Kwong¹

¹Department of Radiology, Massachusetts General Hospital and Harvard Medical School, ²William Beaumont Hospital

FMRI and physiological monitoring is used to study the effects of Acupuncture on the central and peripheral nervous systems. Acupuncture mobilizes a limbic-paralimbic-neocortical network, with great overlap with the default mode network, to modulate neurological activity, possibly related to its autonomic effect in the peripheral nervous system.

JoVE Journal

Fabrication and Testing of Microfluidic Optomechanical Oscillators

Kewen Han¹, Kyu Hyun Kim², Junhwan Kim¹, Wonsuk Lee^2,3, Jing Liu³, Xudong Fan³, Tal Carmon², Gaurav Bahl¹

¹Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, ²Electrical Engineering and Computer Science, University of Michigan, ³Biomedical Engineering, University of Michigan

Parametric optomechanical excitations have recently been experimentally demonstrated in microfluidic optomechanical resonators by means of optical radiation pressure and stimulated Brillouin scattering. This paper describes the fabrication of these microfluidic resonators along with methodologies for generating and verifying optomechanical oscillations.

Bioengineering

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Eric Ehrke-Schulz¹, Wenli Zhang¹, Maren Schiwon¹, Thorsten Bergmann¹, Manish Solanki¹, Jing Liu¹, Philip Boehme¹, Theo Leitner¹, Anja Ehrhardt¹

¹Institute of Virology and Microbiology, Center for Biomedical Education and Research (ZBAF), Department of Human Medicine, Faculty of Health, University Witten/Herdecke

A protocol for generation of high-capacity adenoviral vectors lacking all viral coding sequences is presented. Cloning of transgenes contained in the vector genome is based on homing endonucleases. Virus amplification in producer cells grown as adherent cells and in suspension relies on a helper virus providing viral genes in trans.

JoVE Journal

Combined Genetic and Chemical Capsid Modifications of Adenovirus-Based Gene Transfer Vectors for Shielding and Targeting

Franziska Jönsson¹, Claudia Hagedorn¹, Florian Kreppel¹

¹Center of Biomedical Education and Research, University Witten/Herdecke

The protocol described here enables researchers to specifically modify adenovirus capsids at selected sites by simple chemistry. Shielded adenovirus vectors particles and retargeted gene transfer vectors can be generated, and vector host interactions can be studied.

Medicine

Protocol and Guidelines for Point-of-Care Lung Ultrasound in Diagnosing Neonatal Pulmonary Diseases Based on International Expert Consensus

Jing Liu^1,2, Roberto Copetti³, Erich Sorantin⁴, Jovan Lovrenski⁵, Javier Rodriguez-Fanjul⁶, Dalibor Kurepa⁷, Xing Feng⁸, Luigi Cattaross⁹, Huayan Zhang^10,11, Misun Hwang¹², Tsu F. Yeh^13,14, Yisrael Lipener⁷, Abhay Lodha¹⁵, Jia-Qin Wang¹⁶, Hai-Ying Cao^2,17, Cai-Bao Hu^2,18, Guo-Rong Lyu¹⁹, Xin-Ru Qiu^1,2, Li-Qun Jia²⁰, Xiao-Man Wang²⁰, Xiao-Ling Ren^1,2, Jiu-Ye Guo^1,2, Yue-Qiao Gao^1,2, Jian-Jun Li^1,2, Ying Liu^1,2, Wei Fu^1,2, Yan Wang²¹, Zu-Lin Lu^1,2, Hua-Wei Wang⁸, Li-Li Shang²²

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The Neonatal Lung Ultrasound Training Base, Chinese College of Critical Ultrasound, ³Emergency Department, Cattinara University Hospital, ⁴Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ⁵Faculty of Medicine, University of Novi Sad, Radiology Department, Institute for Children and Adolescents Health Care of Vojvodina, ⁶Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ⁷Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁸Department of Neonatology, Children's Hospital of Soochow University, ⁹Department of Neonatology, Udine University Hospital, ¹⁰Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹¹Division of Neonatology, Children's Hospital of Philadelphia, ¹²Department of Radiology, Children's Hospital of Philadelphia, ¹³Division of Neonatology and NICU, Cook County Children's Hospital, University of Illinois, ¹⁴Division of Neonatology, Department of Pediatrics, Taipei Medical University, ¹⁵Department of Pediatrics and Community Health Sciences, University of Calgary, ¹⁶Department of Pediatrics, The Third Affiliated Hospital of Xinxiang Medical University, ¹⁷Department of Ultrasound, GE Healthcare, ¹⁸Intensive Care Unit, Zhejiang Hospital, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Beijing Children's Hospital Affiliated with Capital Medical University, ²¹Department of Neonatology and NICU, Tai'an City Central Hospital of Shandong Province, ²²Department of Intensive Care Unit, The Second Affiliated Hospital of Heilongjiang University of Chinese Medicine

Lung ultrasound is a noninvasive and valuable tool for bedside evaluation of neonatal lung diseases. However, a relative lack of reference standards, protocols and guidelines may limit its application. Here, we aim to develop a standardized neonatal lung ultrasound diagnostic protocol to be used in clinical decision-making.

JoVE Core

International Expert Consensus and Recommendations for Neonatal Pneumothorax Ultrasound Diagnosis and Ultrasound-guided Thoracentesis Procedure

Jing Liu^1,2, Dalibor Kurepa³, Francesco Feletti^4,5, Almudena Alonso-Ojembarrena⁶, Jovan Lovrenski⁷, Roberto Copetti⁸, Erich Sorantin⁹, Javier Rodriguez-Fanjul¹⁰, Karishma Katti³, Andrea Aliverti⁴, Huayan Zhang^11,12, Misun Hwang¹³, Tsu F. Yeh¹⁴, Cai-Bao Hu¹⁵, Xing Feng¹⁶, Ru-Xin Qiu^1,2, Jing-Han Chi¹⁷, Li-Li Shang¹⁸, Guo-Rong Lyu¹⁹, Shao-Zheng He²⁰, Yan-Fen Chai²¹, Zhan-Jun Qiu²², Hai-Ying Cao^2,23, Yue-Qiao Gao^1,2, Xiao-Ling Ren^1,2, Guo Guo^1,24, Li Zhang^1,2, Ying Liu^1,2, Wei Fu^1,2, Zu-Lin Lu^1,2, Hong-Lei Li^1,2

¹Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, ²The National Neonatal Lung Ultrasound Training Base, ³Division of Neonatal-Perinatal Medicine, Cohen Children's Medical Center, ⁴Department of Electronics, Information and Bioengineering, Politecnico di Milano, ⁵Dipartimento di Diagnostica per Immagini, Ausl della Romagna, S. Maria delle Croci Hospital, ⁶Neonatal Intensive Care Unit, Puerta del Mar University Hospital, ⁷Faculty of Medicine, University of Novi Sad, Serbia, Institute for Children and Adolescents Health Care of Vojvodina, ⁸Emergency Department, University Hospital of Cattinara, ⁹Division of Pediatric Radiology, Department of Radiology, Medical University Graz, ¹⁰Pediatric Intensive Care Unit, Pediatric Service Hospital Joan XXIII Tarragona, University Rovira i Virgil, ¹¹Center for Newborn Care, Guangzhou Women and Children's Medical Center, ¹²Division of Neonatology, Children's Hospital of Philadelphia, ¹³Section of Neonatal Imaging, Department of Radiology, Children's Hospital of Philadelphia, ¹⁴Maternal Child Health Research institute, Taipei Medical University and China Medical University, ¹⁵Intensive Care Unit, Zhejiang Hospital, ¹⁶Department of Neonatology, Children's Hospital of Soochow University, ¹⁷Department of Neonatology and NICU, Bayi Children's Hospital Affiliated to the Seventh Medical Center of Chinese PLA General Hospital, ¹⁸Intensive Care Unit, Second Affiliated Hospital of Heilongjiang University of Chinese Medicine, ¹⁹Collaborative Innovation Center for Maternal and Infant Health Service Application Technology, Quanzhou Medical College, ²⁰Department of Ultrasound, Second Affiliated Hospital of Fujian Medical University, ²¹Department of Emergency Medicine, Tianjin Medical University General Hospital, ²²Department of Emergency and Critical Care Medicine, Affiliated Hospital of Traditional Chinese Medicine, ²³Department of Ultrasound, GE Healthcare, ²⁴The Neonatal Intensive Care Unit, Fifth Medical Center of Chinese PLA General Hospital

Pneumothorax is a common emergency and critical disease in newborn infants that needs rapid, clear diagnosis and timely treatment. Diagnosis and treatment based on chest X-rays are associated with delayed management and radiation damage. Lung ultrasound (US) provides useful guidance for rapid, accurate diagnosis and the precise thoracentesis of pneumothorax.

Immunology and Infection

Contact-Free Co-Culture Model for the Study of Innate Immune Cell Activation During Respiratory Virus Infection

Zhe Zhang Ryan Lew^*1, Jing Liu^*1, Hsiao Hui Ong¹, Vivian Jiayi Tan², Annika Luukkainen³, Yew Kwang Ong^1,4, Mark Thong^1,4, Kia Joo Puan⁵, Vincent Tak Kwong Chow^2,6, Kai Sen Tan^1,2,6, De Yun Wang^1,6

¹Department of Otolaryngology, Yong Loo Lin School of Medicine, National University of Singapore, ²Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ³Haartman Institute, Medicum, University of Helsinki, ⁴Department of Otolaryngology, Head and Neck Surgery, National University Hospital, National University Health System, ⁵Agency for Science, Technology and Research (A*STAR), Singapore Immunology Network (SIgN), ⁶NUHS Infectious Diseases Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

This protocol details an investigation of the early interactions between virally infected nasal epithelial cells and innate cell activation. Individual subsets of immune cells can be distinguished based on their activation in response to viral infections. They can then be further investigated to determine their effects on early antiviral responses.