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Academy of Scientific and Innovative Research (AcSIR)

4 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Generation of Neurospheres from Mixed Primary Hippocampal and Cortical Neurons Isolated from E14-E16 Sprague Dawley Rat Embryo
Gaurav Das 1,2, Varsha Gupta 1, Juhee Khan 1, Deepshikha Mukherjee 1, Surajit Ghosh 1,2
1Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 2Academy of Scientific and Innovative Research (AcSIR)

Presented here is a protocol for the spontaneous generation of neurospheres enriched in neural progenitor cells from high density plated neurons. During the same experiment, when neurons are plated at a lower density, the protocol also results in prolonged primary rat neuron cultures.

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Biology

A Simple Protocol for Mapping the Plant Root System Architecture Traits
Devesh Shukla 1,2, Prabodh Kumar Trivedi 2,3, Shivendra Sahi 1,4
1Department of Biology, Western Kentucky University, 2Plant Biotechnology Division, CSIR Central Institute of Medicinal and Aromatic Plants, 3Academy of Scientific and Innovative Research (AcSIR), 4Department of Biology, St. Joseph's University

We use simple laboratory tools to examine the root system architecture (RSA) of Arabidopsis and Medicago. The plantlets are grown hydroponically over mesh and spread using an art brush to reveal the RSA. Images are taken using scanning or a high-resolution camera, then analyzed with ImageJ to map traits.

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Biology

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera
Sharada D. Mohite 1,2, Yogita P. Patil 1,2, Vikram J. Nichit 1,2, Rakesh S. Joshi 1,2
1Biochemical Sciences Division, CSIR-National Chemical Laboratory, 2Academy of Scientific and Innovative Research (AcSIR)

This protocol describes the obligate feeding assay to evaluate the potentially toxic effect of a phytochemical on the lepidopteran insect larvae. This is a highly scalable insect bioassay, easy to optimize the sublethal and lethal dose, deterrent activity, and physiological effect. This could be used for screening eco-friendly insecticides.

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Biology

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
Anamika Singh 1,2, Mohd Adnan Siddiqui 1, Afzal Azim 3, Neeraj Sinha 1,2
1Centre of Biomedical Research, SGPGIMS Campus, 2Academy of Scientific and Innovative Research (AcSIR), 3Department of Critical Care Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences

Nuclear magnetic resonance (NMR) spectroscopy is used to identify dysregulation in the metabolites in patients with various diseases. This technique allows the quantification of the deranged metabolites, unraveling the pathophysiological insights. Here, we describe the step-by-step procedure of the NMR-based approach for the metabolic characterization of the patients.

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