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INRS, Institut Armand-Frappier

2 ARTICLES PUBLISHED IN JoVE

Biology

Evaluation of Mammary Gland Development and Function in Mouse Models

Isabelle Plante¹, Michael K.G. Stewart¹, Dale W. Laird¹

¹Department of Anatomy and Cell Biology, University of Western Ontario

This method describes how to dissect and assess mammary gland development and function from mice. Excised mammary glands are assessed for the degree of development using whole mount while milk ejection is evaluated using an oxytocin-based myoepithelial cell contraction assay.

Developmental Biology

Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands

Elham Dianati^1,2, Isabelle Plante¹

¹INRS, Institut Armand-Frappier, ²Biomed, UQAM

Intercellular junctions are requisites for mammary gland stage-specific functions and development. This manuscript provides a detailed protocol for the study of protein-protein interactions (PPIs) and co-localization using murine mammary glands. These techniques allow for the investigation of the dynamics of the physical association between intercellular junctions at different developmental stages.