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CIBER de Enfermedades Cardiovasculares

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

Raquel Toribio-Fernandez¹, Virginia Zorita¹, Beatriz Herrero-Fernandez², Jose M. Gonzalez-Granado^1,2,3

¹LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), ²LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), ³CIBER de Enfermedades Cardiovasculares

Here, we present a protocol for the in vivo determination of naïve CD4 T cell (T cell) activation, proliferation, and Th1 differentiation induced by GM-CSF bone marrow (BM)-derived dendritic cells (DCs). In addition, this protocol describes BM and T-cell isolation, DC generation, and DC and T-cell adoptive transfer.

Developmental Biology

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Tamara Gonzalez-Costa^1,2, Jose Luis de la Pompa^1,2, Joaquim Grego-Bessa^1,2

¹Intercellular Signalling in Cardiovascular Development and Disease Laboratory, Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Ciber de Enfermedades Cardiovasculares

Classically, the endocardium of the mouse embryonic valve primordium has been analyzed using transversal, coronal, or sagittal sections. Our novel approach for en face, two-dimensional imaging of the endocardium at valvulogenic regions allows planar polarity and cell rearrangement analysis of the endocardium during valve development.