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Arthur G. James Cancer Hospital and Richard J. Solove Research Institute

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Detection of Protease Activity by Fluorescent Peptide Zymography
Ameya A. Deshmukh 1,2, Jessica L. Weist 1, Jennifer L. Leight 1,2
1Comprehensive Cancer Center, James Cancer Hospital and Solove Research Center, Ohio State University, 2Department of Biomedical Engineering, College of Engineering, Ohio State University

Here, we present a detailed protocol for a modified zymographic technique in which fluorescent peptides are used as the degradable substrate in place of native proteins. Electrophoresis of biological samples in fluorescent peptide zymograms enables detection of a wider range of proteases than previous zymographic techniques.

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Bioengineering

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
Abdulaziz S. Fakhouri 1,2,3, Jennifer L. Leight 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

Here, a protocol is presented for encapsulating and culturing cells in poly(ethylene glycol) (PEG) hydrogels functionalized with a fluorogenic matrix metalloproteinase (MMP)-degradable peptide. Cellular MMP and metabolic activity are measured directly from the hydrogel cultures using a standard microplate reader.

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