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Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Zhenan Liu¹, Jie Wen^1,2, Xue Leng¹, Qian Zhou¹, Changkuo Zhou³, Huaqiang Zhao¹, Xunwei Wu^1,2

¹Shandong Provincial Key Laboratory of Oral Tissue Regeneration and Laboratory for Tissue Engineering and Regeneration, School of Stomatology, Shandong University, ²Suzhou Institute of Shandong University, ³Department of Urology, Qilu Hospital of Shandong University

Here we present a protocol to efficiently isolate primary human keratinocytes from adult skin tissues. This method simplifies the conventional procedure by using the ROCK Inhibitor Y-27632 in the inoculation medium to spontaneously separate epidermal cells from dermal cells.

Biology

Y-27632 Enriches the Yield of Human Melanocytes from Adult Skin Tissues

Chang Liu¹, Shuangshuang Wang¹, Man Liu², Fuxiang Bai¹, Zhihong Chen³, Ping Wang⁴, Jun Mi^1,5, Xunwei Wu¹

This paper reports that the addition of Y-27632 to TIVA medium can significantly increase the yield of melanocytes from adult skin tissues.

Bioengineering

Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632

Zhiwei Xie^1,2,3, Jizhou Shi⁴, Min Zong³, Qiuping Xu¹, Chang Liu¹, Jie Wen¹, Qun Zhang¹, Panpan Liu¹, Guanyi Liu¹, Jing Guo^2,5, Xunwei Wu¹

¹Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ²Department of Orthodontics, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, ³Department of Stomatology, Shengli Oilfield Central Hospital, ⁴Department of Pediatric Surgery, Shengli Oilfield Central Hospital, ⁵Ningbo Stomatology Hospital of Savaid Stomatology School, Hangzhou Medical College

Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.

Bioengineering

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Rang Feng¹, Guo-Cheng Li¹, Hai-Ming Jing¹, Chang Liu¹, Ruo-Yi Xue¹, Quan-Ming Zou¹, Hai-Bo Li¹

¹National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, College of Pharmacy, Third Military Medical University

The present protocol describes the bioengineering of outer membrane vesicles to be a "Plug-and-Display" vaccine platform, including production, purification, bioconjugation, and characterization.