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Brigham and Women’s Hospital, Harvard Medical School

5 ARTICLES PUBLISHED IN JoVE

Bioengineering

Plasma Lithography Surface Patterning for Creation of Cell Networks

Michael Junkin¹, Siu Ling Leung¹, Yongliang Yang¹, Yi Lu¹, Justin Volmering¹, Pak Kin Wong^1,2

¹Aerospace and Mechanical Engineering, University of Arizona , ²Biomedical Engineering IDP and BIO5 Institute, University of Arizona

A versatile plasma lithography technique has been developed to generate stable surface patterns for guiding cellular attachment. This technique can be applied to create cell networks including those that mimic natural tissues and has been used for studying several, distinct cell types.

Medicine

Intraoperative Ultrasound in Spinal Surgery

Melissa M.J. Chua¹, Viren S. Vasudeva¹, Yi Lu¹

¹Brigham and Women's Hospital, Harvard Medical School

Here, we present a protocol on the use of intraoperative ultrasound in spinal surgery, particularly in cases of intradural lesions and lesions in the ventral spinal canal when using a posterior approach.

Immunology and Infection

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (T_HGM) Cells

Yi Lu^1,2,3, Xin-Yuan Fu³, Yongliang Zhang^1,2

¹Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, ²Immunology Programme, Life Sciences Institute, National University of Singapore, ³Cancer Science Institute of Singapore, Yong Loo Lin School of Medicine, National University of Singapore

Here, we present a protocol to differentiate murine granulocyte-macrophage-colony-stimulating-factor-producing T helper (T_HGM) cells from naive CD4+ T cells, including isolation of naive CD4+ T cells, differentiation of T_HGM, and analysis of differentiated T_HGM cells. This method can be applied to studies of the regulation and function of T_HGM cells.

Neuroscience

Three-dimensional Navigation-guided, Prone, Single-position, Lateral Lumbar Interbody Fusion Technique

Samantha E. Hoffman¹, Saksham Gupta¹, Kevin Huang¹, Neil Klinger¹, Yi Lu¹

¹Department of Neurosurgery, Brigham and Women’s Hospital, Harvard Medical School

The single-position, prone, lateral approach allows for both lateral lumbar interbody placement and direct posterior decompression with pedicle screw placement in one position.

Biology

Polycarbonate Ultracentrifuge Tube Re-Use in Proteomic Analyses of Extracellular Vesicles

Rachel M. E. Cahalane^*1,2, Mandy E. Turner^*1, Cassandra L. Clift¹, Mark C. Blaser¹, Gabrielle Bogut¹, Sydney Levy¹, Taku Kasai¹, Tom A. P. Driedonks^3,4, Esther N. M. Nolte-‘t Hoen⁴, Masanori Aikawa^1,5, Sasha A. Singh^1,5, Elena Aikawa^1,5

¹Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, ²Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering, University of Galway, ³Department of CDL Research, University Medical Center Utrecht, ⁴Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, ⁵Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School

A detailed protocol is provided for cleaning and re-using polycarbonate ultracentrifuge tubes to perform extracellular vesicle isolation suitable for proteomics experiments.