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Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Bushra Memon^1,2, Essam M. Abdelalim^1,2

¹College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation, ²Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.

Developmental Biology

Robust Differentiation of Human iPSCs into a Pure Population of Adipocytes to Study Adipocyte-Associated Disorders

Maryam Aghadi^1,2, Manale Karam¹, Essam M. Abdelalim^1,2

¹Diabetes Research Center, Qatar Biomedical Research Institute (QBRI), Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF), ²College of Health and Life Sciences, Hamad Bin Khalifa University (HBKU), Qatar Foundation

The protocol allows the generation of a pure adipocyte population from induced pluripotent stem cells (iPSCs). Retinoic acid is used to differentiate iPSCs into mesenchymal stem cells (MSCs) which are used for producing adipocytes. Then, a sorting approach based on Nile red staining is used to obtain pure adipocytes.