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Université Paris Cité

3 ARTICLES PUBLISHED IN JoVE

Biology

Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Brieuc Chauvin^*1, Koyomi Nakazawa^*1, Alexandre Beber^1,7, Aurélie Di Cicco¹, Bassam Hajj¹, François Iv², Manos Mavrakis², Gijsje H. Koenderink³, João T. Cabral⁴, Michaël Trichet⁵, Stéphanie Mangenot^*6, Aurélie Bertin^*1

¹Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, ²Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, ³Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, ⁴Department of Chemical Engineering, Imperial College London, ⁵Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Service de microscopie électronique (IBPS-SME), ⁶Laboratoire Matière et Systèmes Complexes (MSC), Université Paris Cité, ⁷Institute of Biotechnology, Czech Academy of Sciences, BIOCEV

Septins are cytoskeletal proteins. They interact with lipid membranes and can sense but also generate membrane curvature at the micron scale. We describe in this protocol bottom-up in vitro methodologies for analyzing membrane deformations, curvature-sensitive septin binding, and septin filament ultrastructure.

Medicine

Robot-Assisted Transcanal Endoscopic Ear Surgery for Congenital Cholesteatoma

François Simon^1,2, Yann Nguyen^3,4, Natalie Loundon², Françoise Denoyelle^1,2

¹Université Paris Cité, ²Pediatric Otolaryngology, Head and neck surgery department, AP-HP, Hôpital Necker-Enfants Malades, ³Sorbonne Université, ⁴Otolaryngology - head and neck surgery department AP-HP, Hôpital Pitié-Salpêtrière

This protocol describes the removal of congenital cholesteatoma using minimally invasive transcanal endoscopic ear surgery with a two-handed approach and a robotic endoscope holder.

Medicine

Assessing Intracardiac Vortices with High Frame-Rate Echocardiography-Derived Blood Speckle Imaging in Newborns

Edward Crendal¹, Koert De Waal², Damien Vitiello³

¹John Hunter Hospital, Department of Cardiology, University of Newcastle, ²John Hunter Children’s Hospital, Department of Neonatology, University of Newcastle, ³Institute of Sport and Health Sciences of Paris (IS3P - URP 3625), Université Paris Cité

The present protocol uses echocardiography-derived blood speckle imaging technology to visualize intracardiac hemodynamics in newborns. The clinical utility of this technology is explored, the rotational body of fluid within the left ventricle (known as a vortex) is accessed, and its significance in understanding diastology is determined.