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Children’s Mercy Kansas City

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

Adnan Islam¹, Joshua L. Wheatley², Susana Chavez-Bueno^1,2

¹University of Missouri-Kansas City School of Medicine, ²Pediatric Infectious Diseases, Children’s Mercy Kansas City

Escherichia coli causes sepsis in neonates who ingest the bacteria around the time of birth. The process involved in E. coli’s ability to travel from the enteric tract to the bloodstream is poorly understood. This in vitro model assesses the ability of E. coli strains to travel through the intestinal epithelial cells.

Cancer Research

Real-Time Quantitative Measurement of Tumor Cell Migration and Invasion Following Synthetic mRNA Transfection

Taeju Park^1,2, Neka Large¹

¹Children’s Mercy Research Institute, Children’s Mercy Kansas City, ²Department of Pediatrics, University of Missouri-Kansas City School of Medicine

Many upregulated genes stimulate tumor cell migration and invasion, leading to poor prognosis. Determining which genes regulate tumor cell migration and invasion is critical. This protocol presents a method for investigating the effects of the increased expression of a gene on the migration and invasion of tumor cells in real time.