우리는 그의 정신적 지원을위한 VR 마법의 비디오 클립 박사 Wael 엘 Gendy, 박사 클레멘스 바그너 감사하고 싶습니다. 저자의 개인 자금.

1. 목적 가상 현실 백내장 수술 시뮬레이터와 인간의 눈이 cystotome를 사용하여 구조 기법 아웃 capsulorhexis 방사형 눈물을 설명합니다. 2. 방법 capsulorhexis이 동공 마진 이외의 주변으로 반경 바꾸다 시작하면 다음 단계는 지체없이 적용되어야합니다. <ol><li> 추가 capsulorhexis 박사의 계획을 중지하고 상황을 재평가. </li><li> 안과 viscosurgical 장치 (OVD)과 앞쪽에 챔버를 입력합니다. 우리는 앞쪽에 챔버가 빠르게 reinflated 수 있도록 OVD 들어있는 주사기로 cystotome를 장착하는 것이 좋습니다. </li><li> capsulorhexis 플랩 그런 다음 렌즈 표면에 펼쳐진 남아 있습니다. </li><li> cystotome 팁는 플랩을 절단하거나 펑쳐링 피하기 위해 수평으로 기울어져 있으며, 눈물의 최첨단 근처의 플랩에 종사하지만 너무 눈물의 지점에 가까이하지 않습니다. </li><li> 부드럽게 눈물의 방향에 눈물 반대의 최첨단 밀어하거나 가져옵니다. </li><li> 최고의 찢어진 가장자리는 &#39;U - 턴&#39;을 할 시작합니다. 원하는 궤도에 끊어지고 가장자리를 반환 때까지 플랩에있는 긴장을 유지합니다. </li></ol> 3. 검색 결과 우리의 기술을 사용하여 외과 수술 도구를 변경하지 않고도 방사상 눈물 아웃에 즉시 응답할 수 있습니다. 이 중요 단계에서 수술 도구를 변경하면 인해 갑자기 유리체에서 앞으로 압력의 결과로 앞쪽에 챔버 shallowing을 추가로 방사상 눈물의 위험을 실행합니다. 우리의 기술은 각막 상처 왜곡 이후 앞쪽에 챔버 붕괴를 줄이는 장점도 있습니다.

<ol>
	<li>Little, B. C., Smith, J. H., Packer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> J Cataract Refract Surg. 32 (9), 1420-1422 (2006).</li><li>Neuhann, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Theorie+und+Operationstechnik+der+Kapsulorhexis.">Theorie und Operationstechnik der Kapsulorhexis.</a> Klin Monatsbl Augenheilkd. 190, 542-545 (1987).</li><li>Gimbel, H. V., Neuhann, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development,+advantages+and+methods+of+the+continuous+circular+capsulorhexis+technique.">Development, advantages and methods of the continuous circular capsulorhexis technique.</a> J Cataract Refract Surg. 16, 31-37 (1990).</li><li>Gimbel, H. V., Neuhann, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Continuous+curvilinear+capsulorhexis.+(letter).">Continuous curvilinear capsulorhexis. (letter).</a> J Cataract Refract Sur. 17, 110-111 (1991).</li><li>Gimbel, H. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evolving+techniques+of+cataract+surgery:+continuous+curvilinear+capsulorhexis,+down-slope+sculpting,+and+nucleofractis.">Evolving techniques of cataract surgery: continuous curvilinear capsulorhexis, down-slope sculpting, and nucleofractis.</a> Semin Ophthalmol. 7, 193-207 (1992).</li></ol>

관심 없음 충돌 선언하지 않습니다.

EYESI 외과 시뮬레이터 기술을 복잡한 capsulorhexis 찢어 아웃 연습 외과에 대한 현실적인 교육 플랫폼입니다. Capsulorhexis는 phacoemulsification 및 endocapsular intraocular 렌즈 이식 절차의 가장 중요하고 복잡한 부분입니다. 성공적인 백내장 수술은 좋은 capsulorhexis를 달성에 따라 달라집니다. capsulorhexis 동안, 외과 의사들은 눈물 아웃 capsulorhexis 방사형 같은 어려운 상황에 직면 수 있습니다. 외과 의사는 상황?...

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a novel technique of rescuing capsulorhexis radial tear-out using a cystotome

1 부 : 목적 : 백내장 수술 가상 현실 시뮬레이터와 인간의 눈이 cystotome를 사용하여 구조 기법 아웃 capsulorhexis 방사형 눈물을 설명합니다. 2 부 : 방법 : 단계 : capsulorhexis는 동공 마진 이외의 주변으로 반경 바꾸다 시작하면 다음 단계는 지체없이 적용되어야합니다. 2.1) 추가 capsulorhexis 박사의 계획을 중지하고 상황을 재평가. 2.2) 안과 viscosurgical 장치 (OVD)과 앞쪽에 챔버를 입력합니다. 우리는 앞쪽에 챔버가 빠르게 reinflated 수 있도록 OVD 들어있는 주사기로 cystotome를 장착하는 것이 좋습니다. capsulorhexis의 플랩이 후 남아있는 2.3) 렌즈 표면에 펼쳐진 거죠. 2.4) cystotome 끝 절단 또는 플랩을 펑쳐링 피하기 위해 수평으로 기울어져 있으며, 눈물의 최첨단 근처의 플랩에 종사하지만 너무 눈물의 지점에 가까이하지 않습니다. 2.5) 부드럽게 밀어 또는 눈물의 방향에 눈물 반대의 최첨단을 가져옵니다. 2.6) 선도 끊어지고 가장자리는 &#39;U - 턴&#39;을 할 시작합니다. 원하는 궤도에 끊어지고 가장자리를 반환 때까지 플랩에있는 긴장을 유지합니다. 파트 3 : 검색 결과 : 우리의 기술을 사용하여, 외과 수술 도구를 변경하지 않고도 방사상 눈물 아웃에 즉시 응답할 수 있습니다. 이 중요 단계에서 수술 도구를 변경하면 인해 갑자기 유리체에서 앞으로 압력의 결과로 앞쪽에 챔버 shallowing을 추가로 방사상 눈물의 위험을 실행합니다. 우리의 기술은 각막 상처 왜곡 이후 앞쪽에 챔버 붕괴를 줄이는 장점도 있습니다. 4 부 : 토론 EYESI 외과 시뮬레이터 기술을 복잡한 capsulorhexis 찢어 아웃 연습 외과에 대한 현실적인 교육 플랫폼입니다. Capsulorhexis는 phacoemulsification 및 endocapsular intraocular 렌즈 이식 절차의 가장 중요하고 복잡한 부분입니다. 성공적인 백내장 수술은 좋은 capsulorhexis를 달성에 따라 달라집니다. capsulorhexis 동안, 외과 의사들은 눈물 아웃 capsulorhexis 방사형 같은 어려운 상황에 직면 수 있습니다. 외과 의사는 상황이 더 악화하지 않고 신속하게 문제를 해결하기 위해 학습해야합니다. 상황을 구조의 일부 다른 방법은 capsulorhexis의 집게를 사용하여 설명하고 있습니다. 그러나, 우리는 우리의 방법은, 더 빠르고 더 효과적이고 EYESi 수술 시뮬레이터와 인간의 눈에 보여주으로 조작하기가 쉽습니다 믿습니다. 응답 : 응답 목록 및 자금 소스. 우리는 비디오 클립에 대한 박사 Wael 엘 Gendy을 감사드립니다. 공개 : 우리는 공개할 게 없다는 잠재적인 충돌 관심이나 상태를 설명합니다. 참조 : 1. 브라이언 C. 리틀, 제니퍼 H. 스미스, 마크 패커. 32:1420 1422, 이슈 - 9, J 백내장은 Surg 2006 굴절. 2. Neuhann T. Theorie 놀이 Operationstechnik 데르 Kapsulorhexis. 클린 Monatsbl가 Augenheilkd. 1987, 1990 542-545. 3. 김벌 HV, Neuhann T. 개발, 장점과 지속적인 원형 capsulorhexis 기술의 방법. J 백내장은 Surg을 굴절. 1990, 16 : 31-37. 4. 김벌 HV, Neuhann T. 연속 곡선 capsulorhexis. (편지) J 백내장 쉬르을 굴절. 1991, 17 : 110-111.

Watch this Scientific Journal Video about A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome at JoVE.com

A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome

Capsulorhexis는 phacoemulsification 수술에서 중요한 단계입니다. 외과 의사가 끊어지고 벡터 세력을 제어하여 앞쪽에 렌즈 캡슐에 지속적인 곡선 눈물을 만듭니다. peripherally 확장 눈물은 심각한 합병증이다. 이 동영상은 cystotome를 사용하여 밖으로 capsular 요골 눈물을 구조의 소설 기법을 보여줍니다.

Cystotome를 사용하여 방사형 구조 Capsulorhexis의 소설 기술 테어 아웃

Part 1 : Purpose:   To demonstrate a capsulorhexis radial tear out rescue technique using a cystotome on a virtual reality cataract surgery simulator and ...

a-novel-technique-rescuing-capsulorhexis-radial-tear-out-using

Research

JoVE Journal

Medicine

13.2K 조회수.  Hairmyres Hospital, NHS Lanarkshire. Capsulorhexis는 phacoemulsification 수술에서 중요한 단계입니다. 외과 의사가 끊어지고 벡터 세력을 제어하여 앞쪽에 렌즈 캡슐에 지속적인 곡선 눈물을 만듭니다. peripherally 확장 눈물은 심각한 합병증이다. 이 동영상은 cystotome를 사용하여 밖으로 capsular 요골 눈물을 구조의 소설 기법을 보여줍니다.

비디오: A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome

A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation

We describe a novel non surgical technique to maintain oxygenation and ventilation in a case of difficult intubation and difficult ventilation, which works especially well with poor mask fit.
Can not intubate, can not ventilate" (CICV) is a potentially life threatening situation. In this video we present a simulation of the technique we used in a case of CICV where oxygenation and ventilation were maintained by inserting an endotracheal tube (ETT) nasally down to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.
A 13 year old patient was taken to the operating room for incision and drainage of a neck abcess and direct laryngobronchoscopy. After preoxygenation, anesthesia was induced intravenously. Mask ventilation was found to be extremely difficult because of the swelling of the soft tissue. The face mask could not fit properly on the face due to significant facial swelling as well. A direct laryngoscopy was attempted with no visualization of the larynx. Oxygen saturation was difficult to maintain, with saturations falling to 80%. In order to oxygenate and ventilate the patient, an endotracheal tube was then inserted nasally after nasal spray with nasal decongestant and lubricant. The tube was pushed gently and blindly into the hypopharynx. The mouth and nose of the patient were sealed by hand and positive pressure ventilation was possible with 100% O2 with good oxygen saturation during that period of time. Once the patient was stable and well sedated, a rigid bronchoscope was introduced by the otolaryngologist showing extensive subglottic and epiglottic edema, and a mass effect from the abscess, contributing to the airway compromise. The airway was secured with an ETT tube by the otolaryngologist.This video will show a simulation of the technique on a patient undergoing general anesthesia for dental restorations.

We describe a technique to maintain oxygenation and ventilation using an endotracheal tube inserted nasally to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.

어려운 삽관법 어려운 환기를위한 소설 구조 기술

We describe a novel non surgical technique to maintain oxygenation and ventilation in a case of difficult intubation and difficult ventilation, which ...

연구

의학

A Novel Capsulorhexis Technique Using Shearing Forces with Cystotome

Purpose:
To demonstrate a capsulorhexis technique using predominantly shearing forces with a cystotome on a virtual reality simulator and on a human eye.
Method:
Our technique involves creating the initial anterior capsular tear with a cystotome to raise a flap. The flap left unfolded on the lens surface. The cystotome tip is tilted horizontally and is engaged on the flap near the leading edge of the tear. The cystotome is moved in a circular fashion to direct the vector forces. The loose flap is constantly swept towards the centre so that it does not obscure the view on the tearing edge.
Results:
Our technique has the advantage of reducing corneal wound distortion and subsequent anterior chamber collapse. The capsulorhexis flap is moved away from the tear leading edge allowing better visualisation of the direction of tear. This technique offers superior control of the capsulorhexis by allowing the surgeon to change the direction of the tear to achieve the desired capsulorhexis size.
Conclusions:
The EYESI Surgical Simulator is a realistic training platform for surgeons to practice complex capsulorhexis techniques. The shearing forces technique is a suitable alternative and in some cases a far better technique in achieving the desired capsulorhexis.

This video demonstrates a novel capsulorhexis technique in modern cataract surgery. Capsulorhexis is the most difficult and important part of phacoemulsification surgery.

Cystotome와 쉬어링 부대를 사용하여 소설 Capsulorhexis 기법

Purpose:
To demonstrate a capsulorhexis technique using predominantly shearing forces with a cystotome on a virtual reality simulator and on a human eye....

Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies

The accurate quantification of peripheral neuropathy is important to define at risk patients, anticipate deterioration, and assess new therapies. Conventional methods assess neurological deficits and electrophysiology and quantitative sensory testing quantifies functional alterations to detect neuropathy. However, the earliest damage appears to be to the small fibres and yet these tests primarily assess large fibre dysfunction and have a limited ability to demonstrate regeneration and repair. The only techniques which allow a direct examination of unmyelinated nerve fibre damage and repair are sural nerve biopsy with electron microscopy and skin-punch biopsy. However, both are invasive procedures and require lengthy laboratory procedures and considerable expertise. Corneal Confocal microscopy is a non-invasive clinical technique which provides in-vivo imaging of corneal nerve fibres. We have demonstrated early nerve damage, which precedes loss of intraepidermal nerve fibres in skin biopsies together with stratification of neuropathic severity and repair following pancreas transplantation in diabetic patients. We have also demonstrated nerve damage in idiopathic small fibre neuropathy and Fabry's disease.

Corneal Confocal microscopy is a non-invasive clinical technique which may be used to quantify C fibre damage to diagnose and stratify patients with increasing neuropathic severity.

각막 공촛점 현미경 : 주변 Neuropathies에 작은 광섬유 병리를 정할 소설 비침습 기술

The accurate quantification of peripheral neuropathy is important to define at risk patients, anticipate deterioration, and assess new therapies. ...

A Novel Application of Musculoskeletal Ultrasound Imaging

Ultrasound is an attractive modality for imaging muscle and tendon motion during dynamic tasks and can provide a complementary methodological approach for biomechanical studies in a clinical or laboratory setting. Towards this goal, methods for quantification of muscle kinematics from ultrasound imagery are being developed based on image processing. The temporal resolution of these methods is typically not sufficient for highly dynamic tasks, such as drop-landing. We propose a new approach that utilizes a Doppler method for quantifying muscle kinematics. We have developed a novel vector tissue Doppler imaging (vTDI) technique that can be used to measure musculoskeletal contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities using ultrasound. The goal of this preliminary study was to investigate the repeatability and potential applicability of the vTDI technique in measuring musculoskeletal velocities during a drop-landing task, in healthy subjects. The vTDI measurements can be performed concurrently with other biomechanical techniques, such as 3D motion capture for joint kinematics and kinetics, electromyography for timing of muscle activation and force plates for ground reaction force. Integration of these complementary techniques could lead to a better understanding of dynamic muscle function and dysfunction underlying the pathogenesis and pathophysiology of musculoskeletal disorders.

We describe a new ultrasound-based vector tissue Doppler imaging technique to measure muscle contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities. This approach provides complementary measurements of dynamic muscle function and could lead to a better understanding of mechanisms underlying musculoskeletal disorders.

근골격계 초음파 이미징의 소설 응용 프로그램

Ultrasound is an attractive modality for imaging muscle and tendon  motion during dynamic tasks and can provide a complementary methodological  approach ...

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&#246;m Technique

The percutaneous biopsy technique enables researchers and clinicians to collect skeletal muscle tissue samples. The technique is safe and highly effective. This video describes the percutaneous biopsy technique using a modified Bergstr&#246;m needle to obtain skeletal muscle tissue samples from the vastus lateralis of human subjects. The Bergstr&#246;m needle consists of an outer cannula with a small opening (&#8216;window&#8217;) at the side of the tip and an inner trocar with a cutting blade at the distal end. Under local anesthesia and aseptic conditions, the needle is advanced into the skeletal muscle through an incision in the skin, subcutaneous tissue, and fascia. Next, suction is applied to the inner trocar, the outer trocar is pulled back, skeletal muscle tissue is drawn into the window of the outer cannula by the suction, and the inner trocar is rapidly closed, thus cutting or clipping the skeletal muscle tissue sample. The needle is rotated 90&#176;&#160;and another cut is made. This process may be repeated three more times. This multiple cutting technique typically produces a sample of 100-200 mg or more in healthy subjects and can be done immediately before, during, and after a bout of exercise or other intervention. Following post-biopsy dressing of the incision site, subjects typically resume their activities of daily living right away and can fully participate in vigorous physical activity within 48-72 hr. Subjects should avoid heavy resistance exercise for 48 hr to reduce the risk of herniation of the muscle through the incision in the fascia.

Human Skeletal Muscle Biopsy Procedures Using the Modified Bergstr&amp;#246;m Technique

The purpose of this video is to present the modified Bergstr&#246;m skeletal muscle biopsy technique on human subjects.

수정 된 버그 스트롬 기술을 이용하여 인간의 골격 근육 생검 절차

The percutaneous biopsy technique enables researchers and clinicians to collect skeletal muscle tissue samples. The technique is safe and highly ...

Circumscribed Capsular Infarct Modeling Using a Photothrombotic Technique

Recent increase in the prevalence rate of white matter stroke demands specific research in the field. However, the lack of a pertinent animal model for white matter stroke has hampered research investigations. Here, we describe a novel method for creating a circumscribed capsular infarct that minimizes damage to neighboring gray matter structures. We used pre-surgery neural tracing with adeno-associated virus-green fluorescent protein (AAV-GFP) to identify somatotopic organization of the forelimb area within the internal capsule. The adjustment of light intensity based on different optical properties of gray and white matter contributes to selective destruction of white matter with relative preservation of gray matter. Accurate positioning of optical-neural interface enables destruction of entire forelimb area in the internal capsule, which leads to a marked and persistent motor deficit. Thus, this technique produces highly replicable capsular infarct lesions with a persistent motor deficit. The model will be helpful not only to study white matter stroke (WMS) at the behavioral, circuit, and cellular levels, but also to assess its usefulness for development of new therapeutic and rehabilitative interventions.

This manuscript describes a modeling technique of capsular infarct. Here we utilized a modified photothrombotic technique with low intensity of light after pre-surgery target mapping. Using this technique, we created a circumscribed capsular infarct model with persistent motor impairment.

Photothrombotic 기술을 이용하여 외접 캡슐 경색 모델링

Recent increase in the prevalence rate of white matter stroke demands specific research in the field. However, the lack of a pertinent animal model for ...

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Perspiration monitoring can be utilized for the detection of certain diseases, such as thermoregulation and mental disorders, particularly when the patients are unaware of such disorders or are having difficulty expressing their symptoms. Until now, several devices for perspiration monitoring have been developed; however, such devices tend to have a relatively large exterior, considerable power consumption, and/or less sensitivity.
Recently, we developed a small, wireless device for perspiration monitoring. The device consists of a temperature/relative humidity (T/RH) sensor, battery-driven small data logger, and silica gel as a desiccant in a small cylindrical exterior. The T/RH sensor is placed between the detection windows (through which the water vapor from the skin enters) and the silica gel. The underlying principle of the perspiration monitoring device is based on Fick&#39;s law of diffusion, which means that water vapor flux from the skin to the silica gel (i.e.&#160;transepidermal water loss and perspiration) can be captured by change in humidity at the T/RH sensor. In addition, a baseline subtraction method was adopted to distinguish perspiration and transepidermal water loss.
As shown in the previous report, the developed device can monitor the perspiration at any sites of the body in an easy, wireless manner. However, detailed methods of how to use the device have not been disclosed yet. In this article, therefore, we would like to show the point-by-point tutorials of how to use the device for perspiration monitoring, by showing the sympathetic activity test with the sympathetic skin response monitoring as an example.

Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test.

소설을 사용하여 땀 모니터링에 대한 자세한 프로토콜, 소형, 무선 장치

Perspiration monitoring can be utilized for the detection of certain diseases, such as thermoregulation and mental disorders, particularly when the ...

Mouse Model for Pancreas Transplantation Using a Modified Cuff Technique

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the availability of the widest range of molecular probes and reagents to perform in vivo as well as in vitro studies. Based on our experience with various murine transplantation models, we developed a heterotopic pancreas transplantation model in mice with the intent to analyze mechanisms underlying severe ischemia reperfusion injury-associated early graft damage. In contrast to previously described techniques using suture techniques, herein we describe a new procedure using a non-suture cuff technique. 
In recent years, we have performed more than 300 pancreas transplantations in mice with an overall success rate of &gt;90%, a success rate never described before in mouse pancreas transplantation. The backbone of this non-suture cuff technique for graft revascularization consists of two major steps: (I) pulling the recipient vessel over a polyethylene/polyamide cuff and fixing it with a circumferential ligature, and (II) placing the donor vessel over the everted recipient vessel and fixing it with a second circumferential ligature. The resultant continuity of the endothelial layer results in less thrombogenic lesions with high patency rates and, finally, high success rates.
In this model, arterial anastomosis is achieved by pulling the abdominal aorta of the donor graft over the everted common carotid artery of the recipient animal. Venous drainage of the graft is achieved by pulling the portal vein of the graft over the everted external jugular vein of the recipient. This manuscript provides details and crucial steps of the organ recovery and organ implantation procedures, which will allow researchers with microsurgical skills to perform the transplantation successfully in their laboratories.

Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. This protocol describes a model of murine pancreas transplantation using a non-suture cuff technique, ideally suited for analyzing these early, deleterious damages.

췌 장 이식 수정된 팔목 기술을 사용 하 여 마우스 모델

Mouse models have several advantages in transplantation research, including easy handling, a variety of genetically well-defined strains, and the ...

Minimally Invasive Muscle Embedding (MIME) - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

Skeletal muscle possesses regenerative capacity due to tissue-resident, muscle-fiber-generating (myogenic) satellite cells (SCs), which can form new muscle fibers under the right conditions. Although SCs can be harvested from muscle tissue and cultured in vitro, the resulting myoblast cells are not very effective in promoting myogenesis when transplanted into host muscle. Surgically exposing the host muscle and grafting segments of donor muscle tissue, or the isolated muscle fibers with their SCs onto host muscle, promotes better myogenesis compared to myoblast transplantation. We have developed a novel technique that we call Minimally Invasive Muscle Embedding (MIME). MIME involves passing a surgical needle through the host muscle, drawing a piece of donor muscle tissue through the needle track, and then leaving the donor tissue embedded in the host muscle so that it may act as a source of SCs for the host muscle. Here we describe in detail the steps involved in performing MIME in an immunodeficient mouse model that expresses a green fluorescent protein (GFP) in all of its cells. Immunodeficiency in the host mouse reduces the risk of immune rejection of the donor tissue, and GFP expression enables easy identification of the host muscle fibers (GFP+) and donor-cell-derived muscle fibers (GFP-). Our pilot data suggest that MIME can be used to implant an extensor digitorum longus (EDL) muscle from a donor mouse into the tibialis anterior (TA) muscle of a host mouse. Our data also suggest that when a myotoxin (barium chloride, BaCl2) is injected into the host muscle after MIME, there is evidence of donor-cell-derived myogenesis in the host muscle, with approximately 5%, 26%, 26% and 43% of the fibers in a single host TA muscle showing no host contribution, minimal host contribution, moderate host contribution, and maximal host contribution, respectively.

Minimally Invasive Muscle Embedding MIME - A Novel Experimental Technique to Facilitate Donor-Cell-Mediated Myogenesis

We describe a novel experimental technique that we call Minimally Invasive Muscle Embedding (MIME), which is based on the evidence that skeletal muscle tissue contains viable myogenic cells that can facilitate donor-cell-mediated myogenesis when implanted into a host muscle.

최소한 침략 적 근육 (MIME) 포함-기증자 세포 중재 하는 Myogenesis를 촉진 하기 위하여 새로운 실험 기법

Skeletal muscle possesses regenerative capacity due to tissue-resident, muscle-fiber-generating (myogenic) satellite cells (SCs), which can form new ...

Cardiopulmonary Bypass in a Mouse Model: A Novel Approach

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization and for minimizing organ damage resulting from prolonged extracorporal circulation. The goal of this paper was to demonstrate a fully functional and clinically relevant model of cardiopulmonary bypass in a mouse. We report on the device design, perfusion circuit optimization, and microsurgical techniques. This model is an acute model, which is not compatible with survival due to the need for multiple blood drawings. Because of the range of tools available for mice (e.g., markers, knockouts, etc.), this model will facilitate investigation into the molecular mechanisms of organ damage and the effect of cardiopulmonary bypass in relation to other comorbidities.

This paper describes how to perform cardiopulmonary bypass in mice. This novel model will facilitate the investigation of the molecular mechanisms involved in organ damage.

마우스 모델에서 심폐 바이패스: 새로운 접근

As prolonged cardiopulmonary bypass becomes more essential during cardiac interventions, an increasing clinical demand arises for procedure optimization ...