Name: a detailed protocol for perspiration monitoring using a novel, small, wireless device
Uploaded: 2016-11-24T15:00:00.000+00:00
Duration: 5 min 32 s
Description: Watch this Scientific Journal Video about A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device at JoVE.com

We would like to acknowledge Mr. Ryohei Suganuma and Ms. Sakie Tachibana who helped perform the research. This study was supported in part by the JSPS KAKENHI Grant Numbers 15K20664, 24500848, and 21500405. This study was also funded in part by the MEXT/JST Tenure Track Promotion Program. A part of this study was based on the Japanese Unexamined Patent Application Publication No. 2011-169881 and the Japanese Patent No. 5708911.

주 : 분석의 방법을 포함하는 장치는, 의료 승인하고, 피험자로 실험 프로토콜을 포함한 일본 국 특허 출원 공개 번호 2011-169881 및 일본 특허 제 5708911. 본 연구에 의해 덮여 가나자와 대학의 윤리위원회 (# 553-1). 땀 모니터링 장치 1. 전제 조건 참고 : 처음 사용하기 전에 한 번만 다음 단계를 수행합니다. <ol><li> 컴퓨터 (27)에 범용 직렬 버스 (USB) 포트 - 시리얼 변환 인터페이스 드라이버를 설치한다. 드라이버가 이미 설치되어있는 경우,이 단계를 건너 뜁니다. </li><li> USB 케이블을 통해 컴퓨터에 USB 직렬 포트 변환 인터페이스를 연결합니다. 있는 경우 자동 드라이버 설치를 기다립니다. </li><li> 변환 인터페이스에 매개 변수를 설정하고 다음과 같이 직렬 포트의 ID 번호를 확인한다. <ol><li> 장치 관리자를 열고 선택 [보기]→ [유형에 의하여 장치]. </li><li> 제목 &quot;포트 (COM &amp; LPT)&quot;을 찾아 목록에서 &quot;UART 브리지하는 CP210x의 USB&quot;를 포함하는 제목을 찾을 수 섹션을 확장합니다. </li><li> 은 &quot;UART 브리지하는 CP210x의 USB&quot;호에 포트 (예를 들어, &quot;COM5&quot;)의 ID 번호를 암기. </li><li> 더블 &quot;, UART 브리지하는 CP210x의 USB의&quot;를 클릭하고 &quot;포트 설정&quot;탭을 엽니 다. </li><li> 다음과 같이 매개 변수를 설정합니다. &quot;초당 비트&quot;= 9600, &quot;데이터 비트&quot;= 8, &quot;패리티&quot;= &quot;없음&quot;, &quot;&quot;= &quot;없음 컨트롤을&quot;= 1, 및 &quot;비트를 중지하지 흐름을&quot; </li><li> 창을 닫고, 장치 관리자를 닫습니다 &quot;OK&quot;버튼을 클릭합니다. </li></ol></li></ol> 땀 모니터링 장치 2. 설치 주 : 디바이스를 사용하기 전에 다음과 같은 녹화 설정을 설정한다. 여러 장치를 사용하는 경우 각 장치에 대해 이러한 단계를 반복합니다. <ol><li> 배터리가 땀 감시 장치에 삽입되지 않았는지 확인합니다. </li><li> 발한 감시 장치의 상단에있는 소켓에 USB 직렬 포트 인터페이스 변환 흰색 커넥터 플러그. </li><li> 다음의 장치 매개 변수를 설정 하였다 발한 기록 소프트웨어를 실행한다. <ol><li> &#39;설정&#39;탭을 엽니 다. </li><li> 시리얼 포트의 ID 번호에 &quot;COM&quot;수를 설정한다 (단계 1.3.3 참조). </li><li> &quot;연결 확인&quot;버튼을 클릭합니다. 메시지 &quot;연결이 ...&quot;가 표시되었는지 확인합니다. </li><li> &quot;데이터 파일 폴더&quot;설정에서 드라이브를 선택하고 땀 데이터가 저장 될 폴더를 선택합니다. 하위 폴더를 선택하려면 두 번 폴더를 열려면 클릭합니다. </li><li> &quot;4&quot;에 &quot;구멍의 수&quot;로 설정 </li></ol></li><li> 은 &quot;측정 및 촬영 설정&quot;탭을 열고 원하는 샘플 시간에 &quot;시간 간격&quot;을 설정합니다. </li><li> 배트를 삽입발한 감시 장치 ERY. </li><li> 건조 실리카 젤 장치 (컬러 표시가 가능한 경우 색이 파란색 또는 녹색이어야 함)를 입력하고 뚜껑을 닫습니다. 덮개가 완전히 닫히지 않으면, 실리카 겔의 양을 감소시킨다. </li><li> 땀 레코딩 소프트웨어에서 &quot;지금 로깅 시작&quot;버튼을 클릭합니다. 주 : 발한 기록 막 절단 후에 시작하기 때문에 모니터링이 개시 될 때까지, 발한 감시 장치가 연결된 상태로있다. </li></ol> 교감 신경 피부 반응의 측정 3. 설정 (SSR) 참고 :이 단계는 손바닥 SSR 등의 교감 활동을 모니터하기위한 것입니다, 반드시 땀 모니터링 자체가 필요하지 않습니다. SSR은 화가 농도 (28, 29)과 교감 각성 자극에 따라 피부 전위의 변화이다. <ol><li> SSR이 기록 될 피부를 깨끗하게알코올 면봉으로. </li><li> 전극 용 페이스트를 이용하여 각각의 손등, 손바닥에 양극, 음극, 및 접지 전극을 넣고 손목. 의료 테이프로 전극을 고정합니다. </li><li> 감도 = 1 MV / V, 하이 컷 필터를 ( &quot;HI CUT&quot;) = 3 kHz에서, 그리고 로우 컷 필터 ( &quot;LO CUT&quot;) = 0.5 : 다음과 같이 앰프의 해당 노브를 돌려 계측 증폭기의 조건을 설정 Hz에서. </li><li> SSR의 기록 소프트웨어를 실행하고 소프트웨어의 &quot;측정 시작 &#39;버튼을 클릭하여 200 Hz의 샘플링 레이트에서의 기록을 시작한다. </li></ol> 땀 4. 기록 <ol><li> 땀 감시 장치의 바닥에 의료 양면 테이프를 넣습니다. 테이프를 놓을 때 측정 창 (장치의 하단에, 즉 4 개의 구멍)가 막혀 있지 않은지 확인합니다. </li><li> 금연실,의 USB 직렬 포트에서 땀 감시 장치를 분리rsion 인터페이스를 제공합니다. 즉시 분리 한 후, 땀 모니터링이 자동으로 시작 관찰합니다. 는 LED 램프가 점멸되어 있는지 확인합니다. </li><li> 의료용 양면 테이프의 박리 라이너를 제거하고, 땀을 모니터링 할 수있는 피부에 발한 감시 장치를 넣어. </li><li> 수증기 확산의 안정화를위한 10 분을 기다립니다. 주 :이 프로세스를 모니터링 할 수 있지만, 이전의 연구는 10 분 대기하여 안정적인 감시 26에 충분한 것으로 확인되었다. </li><li> 시험 (예를 들어, 교감 신경 활성 시험)을 시작합니다. </li><li> 시험 후, 피부에서 땀 감시 장치를 제거합니다. SSR의 기록을 중지하려면 SSR 레코딩 ​​소프트웨어에서 &quot;중지 측정&quot;버튼을 클릭하고 피부에서 모든 전극을 제거합니다. </li></ol> 5. 땀 분석 <ol><li> 의 USB 직렬 포트 변환 interfa에 땀 감시 장치를 연결CE (단계 2.2에서와 같이). </li><li> 땀 모니터링 소프트웨어를 실행합니다. </li><li> &#39;설정&#39;탭을 열고 &quot;연결 확인&quot;버튼을 클릭합니다. </li><li> 은 &quot;측정 및 촬영 설정&quot;탭을 열고 지정된 폴더에있는 장치의 원시 데이터를 저장하기 위해 &quot;다운로드&quot;버튼을 클릭한다 (단계 2.3.4 참조). </li><li> 다음과 같이 땀 분석을 수행합니다. <ol><li> 은 &quot;분석&quot;탭을 열고 클릭 &quot;데이터를 읽어 파일을.&quot; &quot;열기 파일&quot;대화 팝 업을 준수하십시오. 단계 5.4에서 저장된 파일을 선택하고 &quot;열기&quot;버튼을 클릭합니다. 주 : 소프트웨어가 자동적으로 문서 (26) 방법에 기초하여 땀의 분석을 수행하고, 결과를 화면에 표시한다. 동시에, 쉼표로 구분 된 값 형식으로 땀 데이터는 같은 폴더에 저장됩니다. </li></ol></li></ol>

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Phys. 38 (4), 391-397 (2016).</li><li>Claus, D., Schondorf, R., Deuschl, G., Eisen, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sympathetic+skin+response.">Sympathetic skin response.</a> Recommendations for the Practice of Clinical Neurophysiology Guidelines of the International Federation of Clinical Physiology (EEG Suppl. 52). , 277-282 (1999).</li><li>Emad, M., Roshanzamir, S., Dabbaghmanesh, A., Ghasempoor, M. Z., Eivazlou, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inclusion+of+Height+and+Limb+Length+when+Interpreting+Sympathetic+Skin+Response.">Inclusion of Height and Limb Length when Interpreting Sympathetic Skin Response.</a> Iran J Med Sci. 41 (1), 48-52 (2016).</li></ol>

The author Fukuoka Masakazu is the president of the Rousette Strategy Inc., where the developed device was assembled. No financial support was received from either Fukuoka Masakazu or the Rousette Strategy Inc.

이 문서의 목적은 신규 무선 땀 감시 장치의 사용을 소개하는 것이다. 기술의 최근 진보에 의해, 시간 발한 모니터링보다 정확하고 취급이 용이 한 방법이 제안되어있다; 통풍 실 (24, 25) 및 방법 증기압 확산법 (23)는 대표적인 예이다. 그러나, 통풍 실 방법은 건조 질소 혹은 건조 분위기를 생성하기 위해 건조제 펌프의 사용을 필요로하고, 이에 따라, 외부 커지는 경향이있다. 증기압 확산법은 개방 챔버 시스템은 주로 외부 환경에 의해 영향을받을 수 있지만, 작은 외부 채용하고 밀폐 챔버 시스템은 수증기 포화 중 하나에 문제 (챔버 포화 수증기로 채워진다 보유 할 수있다 ) 또는 수증기 응축기 상당한 전력 소비 (예를 들어 펠티에 소자). 상황을 해결하기 위해, 우리최근 발한 모니터 (26)에 대한 새로운 무선 장치를 개발했다. 장치에있어서, 피부에서 수증기가 일정하지만, 자연적인 흐름을 가능 건조제에 의해 포착 될 수있다. 수증기 주행하는 과정에서, T / RH 센서는 온도와 상대 습도의 변화와 수증기의 흐름을 캡처한다. 픽의 법률에 따라 이러한 변화를 계산함으로써, 우리는 기존의 환기 챔버 방법 (26)에 비해 땀의 양 &lt;5 % 오차 (밀리그램 / cm 2 / 분)의 시간 변화를 추정 할 수있다. 개발 된 장치는 용이하게하여 정신적 스트레스 (도 2), 열 발한, 궁극적으로 땀과 관련된 조절 곤란을 모니터링하는데 사용될 수있다. 개발 된 장치는 취급이 용이하고, 너무 고려할 몇 점이있다. 실리카겔 건조없는 경우에는 땀 측정 실패. 따라서, 실험 전에0; 심사관은 실리카 겔 (색상이 파란색 즉) 완전히 건조되었는지 확인해야합니다. 이전의 연구는 실리카 겔 (26)을 변경하지 않고 4 시간 이상되는 측정의 최대 지속 기간을 추정 하였다. 색이 청색으로 변할 때까지 실리카 겔을 자연을 다시하기 위해서, 건조 오븐에서 건조시킨다. 종래의 전자 레인지도 유용합니다. 장치의 결합이 충분하지 않은 경우, 땀이 측정은 또한 실패 할 것이다. 우리는 &quot;도넛 모양의&quot;양면 테이프의 사용을 권장합니다. 그럼에도 불구하고, 개발 된 장치는 한계가있다. 때문에 정밀한 계산 및 건조제 흡수율의 한계 때문에, 땀의 양의 오차가 특히 발한의 높은 수준에서 고려되어야한다. 우리가 종래의 방법 (26) &lt;5 %의 상대로서 오류율 확인되었지만, 계산 땀 값의 절대 값은주의 처리되어야. 장치의 소형 단순 무선 설계에 의해, 제한 조건 하에서 발한 지점 모니터링 할 수있다. 도 3에 도시 된 바와 같이, 땀의 다른 시간 프로파일 (등, 음식을 섭취 자동차, 쇼핑, 운전 이야기 등). 생활 조건의 피부 (즉, 손바닥과 전방 가슴)의 서로 다른 위치에서 검출 될 수있다 . 이 장치는, 따라서, 정신 및 열 조절 시스템 오작동으로부터 모두 비정상 발한 유래의 동시 검출을 위해 이용 될 수도있다. 좌우 발바닥 사이의 불균형 땀이 검출한다면 예를 들어, 당뇨병 말초 신경계의 조절 이상을 검출 할 수있다. 우리는 지금 임상 실험으로 입원 환자에 땀 프로파일의 관찰 연구를 계획하고있다.

일반적으로 &quot;땀&quot;로 알려진 인간의 땀은 체온 1 단기구 아니지만, 또한 질환의 특정 유형에 관한 것이다. 열사병, 하이퍼 또는 갑상선 기능 저하증이, 뇌 경색 (3), 당뇨병 (4), 자율 신경 (5), 폐경 ( &quot;핫 플래시&quot;로 알려진) 6, 낭포 성 섬유증 (7), 파킨슨 병 (8), 사회 : 이상 땀의 원인을 포함하여 광범위 불안 장애 (9). 땀 습관병의 수의 관점에서, 그러한 질병의 조기 진단 또는 예측 발한 속도를 모니터링하는 유익한 고려되고있다 (예를 들면, 열사병 예방) 유비쿼터스 방식 십인치 현재까지, 땀 모니터링 장치 소수만이 제안되어있다. 초기에, 피부 전도도와 상대 습도는 간접 내가 사용 하였다땀 (11, 12)의 양 ndices. 이들은 땀 전해질 분석보다는 양이나 땀의 시간적 패턴 의도되지만 최근 발한 모니터링 유연 착용 센서 여러 가지가 13-19 제안되어있다. 수증기 확산 계산 피부 20-23 물 교환을 모니터링하는 양적인 방법에 이용되었다. 그러나이 소모 수증기 (21, 22), 또는 (3)에 냉매 (예를 들어, 펠티에 소자의 자연적인 흐름을 감지하는 (1) 외부의 분위기가 여전히 있음을 가정하고 일정 20 (2)에 충분한 감도를 필요 전기 상당량)을 액체 (23)에 수증기를 응축하는 단계; 따라서, 그들은 매일 장기간 모니터링 어려울 수도있다. 대안으로서, 통풍 실 20,24,25 방법을 개발 하였다. 통풍 실 방법, 건조 질소 또는 제습 공기에서피부로부터 증발 된 수증기의 질소 가스 탱크 나 펌프, 가스의 피부에 인접하는 작은 챔버 내에 침투되어 수집된다. 피부로부터의 수증기의 양은 출구 및 입구 가스의 습도의 차이로부터 계산 될 수있다. 이 방법은 매우 정밀 땀의 양을 추정 할 수 있지만, 질소 가스 탱크 또는 기계적 펌프 충분히 매일 모니터링을 방해하는 것은 일반적으로 크다. 이러한 단점을 해결하기 위해 최근에는 건조제 구동 적용 수증기 흐름 밀폐 챔버 민감한 장기 모니터링 26 활성화되는 땀 모니터링을위한 신규 한 장치를 개발했다. 이 장치는 기록 프로세서, 실리카겔 (도 1)를 원통형 플라스틱 외부 온도 / 상대 습도 (T / RH) 센서로 구성된다. 원칙적으로, 외부 분위기가 수증기 흘러 냉매 또는 ventilatin 방해하지 않아야g 실이 필요하지 않습니다. 땀 프로파일은 스프레드 시트 소프트웨어 (26)를 이용하여 해결하는 식으로 얻을 수있다. 이전의 연구는 현상 장치의 원리를 도시하고 있기 때문에 공간을 제한하는 장치를 사용하는 방법에 대한 구체적인 방법은 생략되었다. 이 문서의 목적은, 따라서, 예로서 동정 활성 시험 동안 스트레스 - 유도 손바닥 땀의 기록을 표시하여, 땀 모니터링을 위해 개발 된 장치를 사용하는 방법에 대한 상세한 방법을 도시한다.

<table><tbody><tr><td>Required for perspiration monitoring</td><td></td><td></td><td></td></tr><tr><td>Perspiration monitoring device</td><td>Rousette Strategy Inc.</td><td>SNT-200</td><td></td></tr><tr><td>USB-serial port conversion interface</td><td>Rousette Strategy Inc.</td><td>UUI-200</td><td></td></tr><tr><td>Perspiration recording software</td><td>Rousette Strategy Inc.</td><td>TED99</td><td></td></tr><tr><td>Silica gel</td><td>Wako Pure Chemical Industries Ltd.</td><td>194-16665</td><td>Type A silica gel should be used.</td></tr><tr><td>Medical double-sided tape</td><td>3M</td><td>2181</td><td>Medical grade is recommended because of the attachment to the skin.</td></tr><tr><td>Computer</td><td></td><td></td><td>Requires Windows operating system.</td></tr><tr><td>&lt;strong&gt;Name&lt;/strong&gt;</td><td>&lt;strong&gt;Company&lt;/strong&gt;</td><td>&lt;strong&gt;Catalog&lt;/strong&gt;</td><td>&lt;strong&gt;Comments&lt;/strong&gt;</td></tr><tr><td>&lt;strong&gt;Required for the monitoring of sympathetic skin response&lt;/strong&gt;</td><td></td><td></td><td></td></tr><tr><td>Instrumentation amplifier</td><td>Nihon Kohden Corp.</td><td>AB-611J</td><td></td></tr><tr><td>Amplifier chassis</td><td>Nihon Kohden Corp.</td><td>MEG-6108</td><td></td></tr><tr><td>Input box</td><td>Nihon Kohden Corp.</td><td>JB-610B</td><td></td></tr><tr><td>Alcohol swab</td><td>Suzuran Sanitary Goods Co., Ltd.</td><td>4545766050846</td><td></td></tr><tr><td>Electrodes</td><td>Nihon Kohden Corp.</td><td>NE-114A</td><td></td></tr><tr><td>Electrode paste</td><td>Nihon Kohden Corp.</td><td>Z-401CE</td><td></td></tr><tr><td>Medical tape</td><td>Nichiban Co., Ltd.</td><td>SG257</td><td></td></tr><tr><td>Analog signal interface</td><td>Micro Science K.K.</td><td>C BOX-014</td><td></td></tr><tr><td>Analog-to-digital converter</td><td>Micro Science K.K.</td><td>ADM-686PCI</td><td></td></tr><tr><td>SSR recording software</td><td>Matsuyama Advance Co., Ltd.</td><td>LaBDAQ2000</td><td></td></tr></tbody></table>

a detailed protocol for perspiration monitoring using a novel, small, wireless device

땀 모니터링이 환자는 질환 또는 모르고 곤란 증상 발현을 데 특히 체온 조절 및 정신 질환과 같은 특정 질환의 검출에 이용 될 수있다. 지금까지 땀 모니터링을위한 여러 장치가 개발되어왔다; 그러나, 이러한 장치는 상대적으로 큰 외부 상당한 전력 소비 및 / 또는 덜 민감도를 갖는 경향이있다. 최근에, 우리는 땀 모니터링을위한 소형 무선 장치를 개발했다. 이 장치는 작은 원통형의 외부에서 건조제로서 온도 / 상대 습도 (T / RH) 센서, 배터리 구동 작은 데이터 로거 실리카겔 구성된다. T 개의 / RH 센서는 검출 창 (통해 피부로부터의 수증기가 유입)하고 실리카겔 사이에 배치된다. 발한 감시 장치의 기본 원리는 수증기 플럭스 F 것을 의미 픽 (Fick)의 확산 법칙에 기초실리카 겔 (예 : 경피 수분 손실 땀)에 피부 ROM하는 T 개의 / RH 센서의 습도 변화에 의해 포착 될 수있다. 또한, 기준 감산 방법이 땀 경피 수분 손실을 구별하기 위해 채택되었다. 이전의 리포트에 도시 된 바와 같이, 현상 장치는 쉬운 무선으로 신체의 어느 부위에서의 발한을 모니터링 할 수있다. 그러나, 장치를 사용하는 방법에 대한 상세한 방법은 아직 개시되지 않았다. 이 글에서, 그러므로, 우리는 예로서 교감 피부 반응 모니터링과 교감 활성 시험을 보여줌으로써, 땀 모니터링 장치를 사용하는 방법의 포인트 별 튜토리얼을 보여 드리고자합니다.

땀 모니터링 (도 1)와 픽의 법칙 기반 연산이 새로운 장치를 사용하여 시간적 땀 프로필 쉬운 무선 방식으로 얻을 수있다. (2)은 교감 활성 시험 중에 무선 땀 모니터링의 대표 데이터를 도시한다. 실험에서, 발한 모니터링 장치는, 교감 신경 피부 반응 (SSR) 모니터링 전극과 함께, 환자의 손바닥에 부착 하였다. 교감 신경 활성 시험의 경우, 피사체가 앉아서 다음 작업을 수행하도록 요청 하였다 : (1) 1 분 간격으로 깊은 감흥을 5 회 수행하고, (2) 암산을 수행 (예를 들어, 연속적으로 (100)로부터 7 뺄셈 참가자 수가 컴퓨터 화면에 차례로 표시 요약되는 &quot;플래시 Anzan&quot;)은 교감 신경 활성을 보여주고있다. 땀과 SSR은 시뮬레이션했다<html> ultaneously 응력 조건에서 기록 하였다. 깊은 영감 암산 결과, 교감 신경 활성 유도 손바닥 땀이 현상 장치를 사용하여 측정 될 수있다. (3)는 일상 활동 중에 다중 측정의 대표 데이터를 도시한다. 손바닥 (감정 땀) 및 전방 가슴 (열 땀)에서 땀 약 1 시간 녹화 활동에 따라 서로 다른 패턴을 보였다. <img alt="그림 1" src="/files/ftp_upload/54837/54837fig1.jpg" /> 도 1 : 발한 모니터링을위한 신규 한 디바이스 (A) 건식 실리카 겔을 포함하는 장치의 외관.. 이 연구와 디바이스의 접속에 사용되는 (B, C) A &quot;도넛 모양&quot;양면 테이프. (D) 피부에의 부착 장치.톰 / 파일 / ftp_upload / 54837 / 54837fig1large.jpg &quot;대상 =&quot;_ 빈 &quot;&gt;이 그림의 더 큰 버전을 보려면 여기를 클릭하십시오. <img alt="그림 2" src="/files/ftp_upload/54837/54837fig2.jpg" /> 교감 활동 테스트에서 땀의 대표적인 기록 교감 피부 반응 (SSR) 손바닥에 전극 땀 감시 장치의 (A) 첨부 파일 : 그림 2.. 교감 신경 활성 시험의 결과 (B)는 SSR 반응은 교감 신경 활동에 대한 응답으로 관찰과 함께 손바닥 땀. <a href="https://www.jove.com/files/ftp_upload/54837/54837fig2large.jpg" target="_blank">이 그림의 더 큰 버전을 보려면 여기를 클릭하십시오.</a> <img alt="그림 3" src="/files/ftp_upload/54837/54837fig3.jpg" /> 그림 3 : <str옹&gt; 데일리 활동시 땀의 대표 지점 기록 신체의 다른 부분에 따라 땀의 독특한 패턴. (손바닥에 빨간 선; 전방 가슴에 파란색 선은) 예를 들어,가는 (여러 활동을하는 동안 관찰되었다 아래층) 이야기와 함께 점심을 먹고, 쇼핑, 차를 운전. <a href="https://www.jove.com/files/ftp_upload/54837/54837fig3large.jpg" target="_blank">이 그림의 더 큰 버전을 보려면 여기를 클릭하십시오.</a>

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A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test.

소설을 사용하여 땀 모니터링에 대한 자세한 프로토콜, 소형, 무선 장치

Perspiration monitoring can be utilized for the detection of certain diseases, such as thermoregulation and mental disorders, particularly when the ...

a-detailed-protocol-for-perspiration-monitoring-using-novel-small

Nanyang Technological University

Research

JoVE Journal

Medicine

7.8K 조회수.  Kanazawa University. Recently, we developed a small wireless device for perspiration monitoring. In this article, we present detailed protocols on how to use the device for perspiration monitoring with an example of the sympathetic activity test.

비디오: A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

A Community-based Stress Management Program: Using Wearable Devices to Assess Whole Body Physiological Responses in Non-laboratory Settings

A pragmatic breath-based intervention to benefit human performance and stress management is timely and valuable to individuals seeking holistic approaches for emotional regulation and optimizing compensatory reserve mechanisms. This protocol is designed to not only teach mind-body awareness but also to provide feedback utilizing physiological data and survey results. The primary findings of this study showed that heart coherence and alpha variables were significantly correlated after four weeks of the breath-based meditation stress protocol. Meditation and rhythmic breathing produced significant increases in alpha brain activity. These brain physiological responses conformed to the Pleth Variability Index (PVI) changes, suggesting the ability of the human body to enter into a meditative state and effectively manage stress. When assessed after four weeks of daily practicing the techniques employed in the stress management protocol, based on the Five Facet Mindfulness Questionnaire, subjects improved in applying mindfulness skills. The overall mindfulness score, Pleth variability index (PVI), and perfusion index (PI) increased after the 4-week intervention period. Results from electroencephalography (brain waves) were consistent with a meditative state during the post-study follow-up session. This provides evidence that wearable devices are feasible for data collection during a breath-based stress management intervention. This protocol can be easily and efficiently implemented into any study design in which physiological data are desired in a non-laboratory-based setting.

Stress is an unavoidable and persistent component of life and holistic approaches for its management are being considered. A standardized methodology was created to demonstrate the feasibility of a breath-based stress management protocol that can be used with commercially available portable technology.

커뮤니티 기반 스트레스 관리 프로그램: 전체를 평가 하기 위해 착용 할 수 있는 장치를 사용 하 여 바디 비 실험실 설정에서 생리 적인 응답

A pragmatic breath-based intervention to benefit human performance and stress management is timely and valuable to individuals seeking holistic approaches ...

연구

행동분석학

Methodology for Establishing a Community-Wide Life Laboratory for Capturing Unobtrusive and Continuous Remote Activity and Health Data

An end-to-end suite of technologies has been established for the unobtrusive and continuous monitoring of health and activity changes occurring in the daily life of older adults over extended periods of time. The technology is aggregated into a system that incorporates the principles of being minimally obtrusive, while generating secure, privacy protected, continuous objective data in real-world (home-based) settings for months to years. The system includes passive infrared presence sensors placed throughout the home, door contact sensors installed on exterior doors, connected physiological monitoring devices (such as scales), medication boxes, and wearable actigraphs. Driving sensors are also installed in participants' cars and computer (PC, tablet or smartphone) use is tracked. Data is annotated via frequent online self-report options that provide vital information with regard to the data that is difficult to infer via sensors such as internal states (e.g., pain, mood, loneliness), as well as data referent to activity pattern interpretation (e.g., visitors, rearranged furniture). Algorithms have been developed using the data obtained to identify functional domains key to health or disease activity monitoring, including mobility (e.g., room transitions, steps, gait speed), physiologic function (e.g., weight, body mass index, pulse), sleep behaviors (e.g., sleep time, trips to the bathroom at night), medication adherence (e.g., missed doses), social engagement (e.g., time spent out of home, time couples spend together), and cognitive function (e.g., time on computer, mouse movements, characteristics of online form completion, driving ability). Change detection of these functions provides a sensitive marker for the application in health surveillance of acute illnesses (e.g., viral epidemic) to the early detection of prodromal dementia syndromes. The system is particularly suitable for monitoring the efficacy of clinical interventions in natural history studies of geriatric syndromes and in clinical trials.

Unobtrusive sensors and pervasive computing technology incorporated into the daily home life of older adults enables meaningful health and activity changes to be recorded continuously for months to years, providing ecologically valid, high frequency, multi-domain data for research or clinical use.

눈에 거슬리지 하 고 지속적인 원격 활동 및 건강 데이터를 캡처에 대 한 사회 전반의 생활 연구소 설립을 위한 방법론

An end-to-end suite of technologies has been established for the unobtrusive and continuous monitoring of health and activity changes occurring in the ...

A Novel Digital Platform for a Monitored Home-based Cardiac Rehabilitation Program

Despite the evidence that cardiac rehabilitation (CR) reduces the risk of recurrent cardiac events, only a minority of eligible patients are willing to join existing programs at cardiac rehabilitation centers. The unique remote patient monitoring system presented here enables healthcare providers to monitor CR patients at home in real-time and at low cost. The system combines mobile technology, artificial intelligence, and supportive services, expanding the delivery of medical care to the patient&#39;s home. The primary aim of the study is to increase the long-term adherence to physical activity in patients who participate in CR via the addition of a home-based digitally monitored CR component to the standard CR program in patients with ischemic heart disease (IHD), with the idea of forming new habitual health behaviors and increasing the long-term motivation for physical exercise (PE) habits at home. Secondary aims are to assess the program&#39;s impact on the physical activity level measured by average steps per day, minutes of exercise per week, blood pressure, metabolic parameters, body mass index, and waist-to-hip ratio, as well as a quality-of-life (QoL) questionnaire.The study has two arms: (1) home-based monitored exercise using a smart digital garment and wristband, in addition to motivation and reinforcement via text messages; (2) standard CR facility-based exercise. The study design is a randomized, controlled trial comparing standard CR to a home-based monitoring and reinforcement program. The study program is designed for 12 weeks.Clinical tests and anthropometric measurements are performed before and after the study, measuring height, weight, waist circumference, visceral fat and body mass index (BMI), blood pressure, and HbA1c and lipid profile. Patients have to complete a baseline survey including socio-demographic characteristics and QoL questionnaire SF-36. At the end of the study, patients complete a survey regarding the use of the smart digital garment&#39;s benefits and usability. The study is currently underway.

The aim is to promote a new approach to cardiac rehabilitation (CR), using a unique remote patient monitoring system that will enable healthcare providers to monitor CR patients at home at low cost, with the intention of making CR services more accessible and improving compliance. The study is currently underway.

Despite the evidence that cardiac rehabilitation (CR) reduces the risk of recurrent cardiac events, only a minority of eligible patients are willing to ...

Evaluation of Commercial-Off-The-Shelf Wrist Wearables to Estimate Stress on Students

Wearable commercial-off-the-shelf (COTS) devices have become popular during the last years to monitor sports activities, primarily among young people. These devices include sensors to gather data on physiological signals such as heart rate, skin temperature or galvanic skin response. By applying data analytics techniques to these kinds of signals, it is possible to obtain estimations of higher-level aspects of human behavior. In the literature, there are several works describing the use of physiological data collected using clinical devices to obtain information on sleep patterns or stress. However, it is still an open question whether data captured using COTS wrist wearables is sufficient to characterize the learners' psychological state in educational settings. This paper discusses a protocol to evaluate stress estimation from data obtained using COTS wrist wearables. The protocol is carried out in two phases. The first stage consists of a controlled laboratory experiment, where a mobile app is used to induce different stress levels in a student by means of a relaxing video, a Stroop Color and Word test, a Paced Auditory Serial Addition test, and a hyperventilation test. The second phase is carried out in the classroom, where stress is analyzed while performing several academic activities, namely attending to theoretical lectures, doing exercises and other individual activities, and taking short tests and exams. In both cases, both quantitative data obtained from COTS wrist wearables and qualitative data gathered by means of questionnaires are considered. This protocol involves a simple and consistent method with a stress induction app and questionnaires, requiring a limited participation of support staff.

A protocol to evaluate solutions based on commercial-off-the-shelf (COTS) wrist wearables to estimate stress in students is proposed. The protocol is carried out in two phases, an initial laboratory-based stress induction test, and a monitoring stage taking place in the classroom while the student is performing academic activities.

상업-오프-더-선반 손목 Wearables 추정 학생에 대 한 스트레스의 평가

Wearable commercial-off-the-shelf (COTS) devices have become popular during the last years to monitor sports activities, primarily among young people. ...

공학

An Application for Pairing with Wearable Devices to Monitor Personal Health Status

The current protocol aims to showcase the technology integration, providing a detailed description of adopting the HealthCloud app, developed by the Healthy Landscape and Healthy People Lab, National Taiwan University (HLHP-NTU), on smartphones and smartwatches to collect data on users' real-time psychological and physiological responses and environmental information. A flexible and integrated research method was proposed because it can be difficult to measure multi-dimensional aspects of personal data in on-site studies in landscape and outdoor recreation research. An on-site study conducted in 2020 at the National Taiwan University campus was used as an application example. A dataset of 385 participants was used after excluding invalid samples. During the experiment, participants were asked to walk around campus for 30 min when their heart rate and psychological-scale items were measured, together with several environmental metrics. This work aimed to provide a possible solution to help on-site studies track real-time human responses that match ambient factors. Due to the app's flexibility, its use on wearable devices shows excellent potential for multidisciplinary research studies.

The present protocol introduces a non-commercial self-developed application for collecting real-time on-site data, including psychological scales, GPS location, heart rate, and blood-oxygen saturation level, as well as the application's operating procedures. An empirical study conducted in Taiwan in 2020 was used as an application example.

개인 건강 상태를 모니터링하기 위해 웨어러블 장치와 페어링하는 응용 프로그램

The current protocol aims to showcase the technology integration, providing a detailed description of adopting the HealthCloud app, developed by the ...

환경과학

Fabrication and Characterization of a Conformal Skin-like Electronic System for Quantitative, Cutaneous Wound Management

Recent advances in the development of electronic technologies and biomedical devices offer opportunities for non-invasive, quantitative assessment of cutaneous wound healing on the skin. Existing methods, however, still rely on visual inspections through various microscopic tools and devices that normally include high-cost, sophisticated systems and require well trained personnel for operation and data analysis. Here, we describe methods and protocols to fabricate a conformal, skin-like electronics system that enables conformal lamination to the skin surface near the wound tissues, which provides recording of high fidelity electrical signals such as skin temperature and thermal conductivity. The methods of device fabrication provide details of step-by-step preparation of the microelectronic system that is completely enclosed with elastomeric silicone materials to offer electrical isolation. The experimental study presents multifunctional, biocompatible, waterproof, reusable, and flexible/stretchable characteristics of the device for clinical applications. Protocols of clinical testing provide an overview and sequential process of cleaning, testing setup, system operation, and data acquisition with the skin-like electronics, gently mounted on hypersensitive, cutaneous wound and contralateral tissues on patients.

This article presents methods to fabricate and characterize a conformal, skin-like electronic system and protocols for the use in clinical applications, particularly on cutaneous wound management.

양적, 피부가 상처 관리를위한 등각 피부와 같은 전자 시스템의 제조 및 특성

Recent advances in the development of electronic technologies and biomedical devices offer opportunities for non-invasive, quantitative assessment of ...

생체공학

Wireless Intramuscular EMG System for Locomotion Studies in Stroke Model Rats

A Real-Time Wearable Electromyography Measurement System for Small Animals

The intramuscular electromyography (EMG) measurement method for experimental animals has been implemented in various ways. Among these methods, tethering cables to external measurement devices can restrict the movement of experimental animals, while implantable devices may cause unwanted side effects due to the constant presence of a device with considerable size and weight. To address these issues, we propose a low-cost, wireless, detachable EMG measurement system and experimental procedure. This article focuses on the surgical installation of intramuscular wire electrodes with small connectors and the development of the wireless system. Notably, in this system, only the wire electrodes are inserted into the animal's body. Using this system, EMG measurements can be easily performed by attaching the circuit system to a connector installed on the animal's back, with real-time monitoring achievable on a laptop. The proposed method is explained in a detailed, step-by-step manner, followed by a demonstration involving the insertion of intramuscular electrodes into the hindlimbs of a rat. A treadmill experiment is conducted for a locomotion study, and the resulting electrophysiological signals are subsequently obtained and analyzed.

A protocol is presented for the installation and preparation of intramuscular electrodes, along with an animal-mountable, miniaturized measurement device for electromyography (EMG) analysis in locomotion studies. This system enables the wireless transmission of real-time EMG data for investigating locomotion recovery in stroke model rats.

The intramuscular electromyography (EMG) measurement method for experimental animals has been implemented in various ways. Among these methods, tethering ...

Home-Based Monitor for Gait and Activity Analysis

Current outcomes in neuromuscular disorder clinical trials include motor function scales, timed tests, and strength measures performed by trained clinical evaluators. These measures are slightly subjective and are performed during a visit to a clinic or hospital and constitute therefore a point assessment. Point assessments can be influenced by daily patient condition or factors such as fatigue, motivation, and intercurrent illness. To enable home-based monitoring of gait and activity, a wearable magneto-inertial sensor (WMIS) has been developed. This device is a movement monitor composed of two very light watch-like sensors and a docking station. Each sensor contains a tri-axial accelerometer, gyroscope, magnetometer, and a barometer that record linear acceleration, angular velocity, the magnetic field of the movement in all directions, and barometric altitude, respectively. The sensors can be worn on the wrist, ankle, or wheelchair to record the subject&#8217;s movements during the day. The docking station enables data uploading and recharging of sensor batteries during the night. Data are analyzed using proprietary algorithms to compute parameters representative of the type and intensity of the performed movement. This WMIS can record a set of digital biomarkers, including cumulative variables, such as total number of meters walked, and descriptive gait variables, such as the percentage of the most rapid or longest stride that represents the top performance of patient over a predefined period of time.

This innovative device uses magneto-inertial sensors to permit gait and activity analysis in uncontrolled environments. Currently in the qualification process as an outcome measure in the European Medical Agency, one of the applications will be to serve as a clinical endpoint in clinical trials in neuromuscular diseases.

걸음걸이 및 활동 분석을 위한 홈 기반 모니터

Current outcomes in neuromuscular disorder clinical trials include motor function scales, timed tests, and strength measures performed by trained clinical ...

Conformable Wearable Electrodes: From Fabrication to Electrophysiological Assessment

Wearable electronic devices are becoming key players in monitoring the body signals predominantly altered during physical activity tracking. Considering the growing interest in telemedicine and personalized care driven by the rise of the Internet of Things era, wearable sensors have expanded their field of application into healthcare. To ensure the collection of clinically relevant data, these devices need to establish conformable interfaces with the human body to provide high-signal-quality recordings and long-term operation. To this end, this paper presents a method to easily fabricate conformable thin tattoo- and soft textile-based sensors for their application as wearable organic electronic devices in a broad spectrum of surface electrophysiological recordings. 
The sensors are developed through a cost-effective and scalable process of cutaneous electrode patterning using poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate) (PEDOT:PSS), the most popular conductive polymer in bioelectronics, on off-the-shelf, wearable substrates. This paper presents key steps in electrode characterization through impedance spectroscopy to investigate their performance in signal transduction when coupled with the skin. Comparative studies are required to position the performance of novel sensors with respect to the clinical gold standard. To validate the fabricated sensors' performance, this protocol shows how to perform various biosignal recordings from different configurations through a user-friendly and portable electronic setup in a laboratory environment. This methods paper will allow multiple experimental initiatives to advance the current state of the art in wearable sensors for human body health monitoring.

Two recent technologies-tattoo and textiles-have demonstrated promising results in cutaneous sensing. Here, we present the fabrication and evaluation methods of tattoo and textile electrodes for cutaneous electrophysiological sensing. These electronic interfaces made of conductive polymers outperform the existing standards in terms of comfort and sensitivity.

적합성 웨어러블 전극: 제작에서 전기생리학적 평가에 이르기까지

Wearable electronic devices are becoming key players in monitoring the body signals predominantly altered during physical activity tracking. Considering ...

Setup of Consumer Wearable Devices for Exposure and Health Monitoring in Population Studies

Wearable sensors, which are often embedded in commercial smartwatches, allow for continuous and non-invasive health measurements and exposure assessments in clinical studies. Nevertheless, the real-life application of these technologies in studies involving a large number of participants for a significant observation period may be hindered by several practical challenges. 
In this study, we present a modified protocol from a previous intervention study for the mitigation of health effects from desert dust storms. The study involved two distinct population groups: asthmatic children aged 6-11 years and elderly patients with atrial fibrillation (AF). Both groups were equipped with a smartwatch for the assessment of physical activity (using a heart rate monitor, pedometer, and accelerometer) and location (using GPS signals to locate individuals in indoor "at home" or outdoor microenvironments). The participants were required to wear the smartwatch equipped with a data collection application on a daily basis, and data were transmitted via a wireless network to a centrally administered data collection platform for the near real-time assessment of compliance. 
Over a period of 26 months, more than 250 children and 50 patients with AF participated in the aforementioned study. The main technical challenges identified included restricting access to standard smartwatch features, such as gaming, internet browser, camera, and audio recording applications, technical issues, such as loss of GPS signal, especially in indoor environments, and the internal smartwatch settings interfering with the data collection application.
The aim of this protocol is to demonstrate how the use of publicly available application lockers and device automation applications made it possible to address most of these challenges in a simple and cost-effective way. In addition, the inclusion of a Wi-Fi received signal strength indicator significantly improved indoor localization and largely minimized GPS signal misclassification. The implementation of these protocols during the roll-out of this intervention study in the spring of 2020 led to significantly improved results in terms of data completeness and data quality.

Commercial smartwatches equipped with wearable sensors are increasingly being used in population studies. However, their utility is often constrained by their limited battery duration, memory capacity, and data quality. This report provides examples of cost-effective solutions to real-life technical challenges encountered during studies involving asthmatic children and elderly cardiac patients.

인구 연구에서 노출 및 건강 모니터링을 위한 소비자 웨어러블 장치 설정

Wearable sensors, which are often embedded in commercial smartwatches, allow for continuous and non-invasive health measurements and exposure assessments ...