이 연구는과 Ethicon Biosurgery 주식 회사 뉴저지, 미국의 &#39;비엔나의 도시의 주요의 의료 과학 기금&#39;에 의해 지원되었다. 저자는 교수 헤버 Ferraz - Leite, 그의 가치 microsurgical 교육에 대한 &#39;미세에서 유럽 워크샵 및 대뇌 Revascularization&#39;비엔나, 오스트리아의 의과 대학에서 이사하기 위해 감사를 표현한다.

1. 위치 및 동물의 준비 실험은 책임 지역 윤리위원회에 의해 승인되고 Felasa 지침에 따라 수행되었다. <ol><li> 여성의 뉴질랜드 화이트 Ketamin의 근육내 주사와 토끼 (2.5-3.5 kg) (30mg/kg)과 Xylazine (6mg/kg)의 전신 마취를 시작하고 (가스 마취 기계 삽관법 (튜브 직경 4mm, 길이 18mm) 이후 계속 ECG 모니터링 아래 Isoflurane). 발가락 핀치 모든 십오분를 통해 고통 모니터링을 수행하고 긍정적인 반응의 경우 마취를 적응. </li><li> bodywarm 접시에 위로 향한 위치에 토끼를 수정. </li><li> 흉부의 중간에 아래 턱뼈의 각도에서 접근 머리카락을 제거하여 수술에 대한 영역을 준비합니다. 무균 조건 하에서 모든 절차를 수행합니다. </li></ol> 2. 외부 경정맥 정맥의 준비 <ol><li> 아래 manubrium sterni에 턱 각도에서 정중선 절개를 수행합니다. </li><li> 수술 현미경을 사용하여 정맥 가지 않고 왼쪽 외부 경정맥 정맥의 1cm 긴 세그먼트를 선택하고 (그림 1A) microsurgically 그것을 분리. 전체 작업하는 동안 자주 4퍼센트 papaverin HCL 솔루션과 vasospasm과 지역​​ 감염을 방지하기 위해 몸에 붙이기도 혈관과 anastomoses에 항생 솔루션 (네오 마이신 황산의 5mg/ml)을 적용합니다. </li><li> 4-0 polyfilament의 봉합 (Vicryl, Ethicon 병원, 뉴저지, 미국)와 함께 proximally와 distally 정맥을 Ligate 다음 그것을 잘라 내다 및 (20 ML 0.9 %의 식염수 1 ML 4퍼센트 papaverin HCL 1000 IU의 헤파린 heparinized 호수에 보관 ). </li></ol> 3. 둘 다 일반적인 경동맥 동맥 (CCAs)의 준비 <ol><li> 대동맥 아치 아래로 경동맥 분기점에서 도달 왼쪽 CCA의 세그먼트를 준비합니다. 가능한 동맥 가지가 medially 실행 및 후두 및 tracheal 구조뿐만 아니라 모든 신경 구조를 제공 한까지 유지하고있다. </li><li> 오른쪽 CCA를 준비합니다. 경동맥 분기점과 아래 bracchiocephalic 트렁크에까지 동원. </li><li> 정맥 1,000 IU의 헤파린을 관리할 수 있습니다. </li><li> 일시적으로 바로 경동맥 분기점 아래 오른쪽 CCA를 클립. </li><li> proximally 직접 brachiocephalic 트렁크 위에 오른쪽 CCA를 Ligate. 끈 위의 오른쪽 CCA를 잘라 heparinized 호수와 몸통 관개. </li><li> 오른쪽 CCA의 무료 끝에 adventitia 잘라 조심스럽게 잘라 내다. 자유는 꼼꼼하게 왼쪽 CCA의 세그먼트는 adventitia의 문합 계획 및 distally 및 proximally을 클립. </li></ol> 4. 두 CCAs의 문합 <ol><li> 오른쪽 CCA와 정맥 주머니와 계획 문합의 크기에 따라 클립 사이에 왼쪽 CCA의 타원형 arteriotomy을 수행합니다. heparinized 식염수에 왼쪽 CCA를 관개. (그림 1B) </li><li> 4-5 비 resorbable 10-0 monofilament의 봉합 (Ethilon 10-0, Ethicon 병원, 뉴저지, 미국)를 사용하여, 왼쪽 CCA의 arteriotomy에 오른쪽 CCA - 유세의 사후 둘레를 봉합해. </li><li> 정맥 주머니의 절반 원주에 적응 권리 CCA의 그루터기에 세로 절개를합니다. 다시 4-5 10-0 봉합을 사용하여 왼쪽 CCA의 arteriotomy로 먼저 정맥 주머니 벽의 뒷면을 Anastomose. 그런 다음 3-4 봉합과 오른쪽 CCA 뒷면과 정맥 파우치의 뒷면을 anastomose. (그림 1C) </li><li> 앞쪽에 측면에서 같은 순서로 문합을 수행합니다. (그림 1D) </li><li> 오른쪽 CCA에있는 말초 클립을 제거합니다. 동맥류는 일반적으로 완전히 밀폐되지 않습니다, 그러므로 갇힌 공기와 파편이 밖으로 씻는다. </li><li> 지방 패드와 섬유소 접착제 (Evicel, Ethicon Biosurgery 주식, 뉴저지, 미국)와 문합 주변의 봉합 라인과 동맥류의 목을 인감. 나머지 클립을 제거합니다. </li><li> 실행중인 cutaneus의 봉합을 위해 다음 중단 피하 봉합과에 대해 4-0 resorbable 봉합 (Monocryl, Ethicon 병원, 뉴저지, 미국)를 사용합니다. </li></ol> 5. 수술후 관리 <ol><li> 정맥 10 MG / kg 아세틸 살리실산, 그리고 subcutaneously 60 ML 5 % 포도당을 관리할 수 있습니다. </li><li> 72시간 동안 효과적인 진통제에 대한 동물의 면도 목 지역에서 12.5 μg / 시간을 발표 transdermal 펜타 닐 매트릭스 패치를 적용합니다. </li><li> 2 주 동안 매일 250 IU subcutaneously / kg 낮은 분자 헤파린을 관리할 수 있습니다. </li></ol> 6. 대표 결과 : 절차가 올바르게 완료되면 동맥류가 특허를 유지하고 동물에 대해 3 일 이내에 회복됩니다. 우리의 경험에서 이미징 절차는 한 일주일 뒤 최소한 완료해야합니다. 그렇지 않으면 동물 가능성이 절차를 용납하지 않을 것이며 손실 수 있습니다. embolization을 위해 우리는 복잡한를 보장하기 위해 약 4 주 동안 기다려야 권장제공 기술 및 관리 저자 neck.Using 동맥류의 테의 endothelialization는 최근에 출판된 일련의 동맥류의 85.7 %에 명백없이 죽음에 도달 수 있습니다. 14 20 %까지보고 창자 합병증의 높은 비율을 최소화하기 위해, 우리가 제공하는 12 일상적인 24 시간 analgesia.This 강화 관리에 비해 72 시간 진통제의 장기 수술 analgetic 관리없이 위장 합병증 결과. 적극적인 anticoagulation 관리 충분한 동맥 명백 속도에 도달하는 것이 중요합니다. 이것은 그런왈드 외의 결과와 함께 간다. 통합 anticoagulation의 긍정적인 효과를 보여주는. 17 그것이 적극적인 anticoagulation 때문에 문합의 효과적인 씰링을 가지고 결정. 우리는 새로운 섬유소 아교를 (Evicel, Ethicon Biosurgery 주식, 뉴저지, 미국)를 사용하여 아주 좋은 경험을했다. 또한 우리는 종종 과체중 주제와 관련된 경험의 합병증과 같이, 작은 토끼 (최대 3.5 kg)을 사용하는 것이 좋습니다.

<ol>
	<li>Molyneux, A., Kerr, R., Yu, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=International+subarachnoid+aneurysm+trial+(ISAT)+of+neurosurgical+clipping+versus+endovascular+coiling+in+2143+patients+with+ruptured+intracranial+aneurysms:+a+randomised+comparison+of+effects+on+survival,+dependency,+seizures,+rebleeding,+subgroups,+and+aneurysm+occlusion.">International subarachnoid aneurysm trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised comparison of effects on survival, dependency, seizures, rebleeding, subgroups, and aneurysm occlusion.</a> Lancet. 366, 809-817 (2005).</li><li>Mitchell, P., Kerr, R., Mendelow, A., Molyneux, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Could+late+rebleeding+overturn+the+superiority+of+cranial+aneurysm+coil+embolization+over+clip+ligation+seen+in+the+International+Subarachnoid+Aneurysm+Trial.">Could late rebleeding overturn the superiority of cranial aneurysm coil embolization over clip ligation seen in the International Subarachnoid Aneurysm Trial.</a> J Neurosur. 108, 437-442 (2008).</li><li>Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histopathological+findings+in+experimental+aneurysms+embolized+with+conventional+and+thrombogenic/antithrombolytic+Guglielmi+coils.">Histopathological findings in experimental aneurysms embolized with conventional and thrombogenic/antithrombolytic Guglielmi coils.</a> Minim Invasive Neurosurg. 42, 167-174 (1999).</li><li>Bocher-Schwarz, H. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histological+findings+in+coil-packed+experimental+aneurysms+3+months+after+embolization.">Histological findings in coil-packed experimental aneurysms 3 months after embolization.</a> Neurosurgery. 50, 379-384 (2002).</li><li>Sherif, C., Plenk, H., Grossschmidt, K., Kanz, F., Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Computer-assisted+quantification+of+occlusion+and+coil+densities+on+angiographic+and+histological+images+of+experimental+aneurysms.">Computer-assisted quantification of occlusion and coil densities on angiographic and histological images of experimental aneurysms.</a> Neurosurgery. 58, 559-566 (2006).</li><li>Massoud, T., Guglielmi, G., Ji, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experiemental+saccular+aneurysms.+1.+Review+of+surgically+constructed+models+and+their+laboratory+applications.">Experiemental saccular aneurysms. 1. Review of surgically constructed models and their laboratory applications.</a> Neurorad. 36, 537-546 (1994).</li><li>Anidjar, S., Salzmann, J., Gentric, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase+induced+experimental+aneurysms+in+rats.">Elastase induced experimental aneurysms in rats.</a> Circulation. 82, 973-981 (1990).</li><li>Wakhloo, A., Schellhammer, F., de Vries, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Self-expanding+and+balloon-expandable+stents+in+the+treatment+of+carotid+aneurysms:+an+experimental+study+in+a+canine+model.">Self-expanding and balloon-expandable stents in the treatment of carotid aneurysms: an experimental study in a canine model.</a> AJNR. 15, 493-502 (1994).</li><li>Dai, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histopathologic+and+immunohistochemical+comparison+of+human,+rabbit,+and+swine+aneurysms+embolized+with+platinum+coils.">Histopathologic and immunohistochemical comparison of human, rabbit, and swine aneurysms embolized with platinum coils.</a> AJNR Am J Neuroradiol. 26, 2560-2568 (2005).</li><li>Shin, Y. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Creation+of+four+experimental+aneurysms+with+different+hemodynamics+in+one+dog.">Creation of four experimental aneurysms with different hemodynamics in one dog.</a> AJNR. 26, 1764-1767 (2005).</li><li>Abruzzo, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histologic+and+morphologic+comparison+of+experimental+aneurysms+with+human+intracranial+aneurysms.">Histologic and morphologic comparison of experimental aneurysms with human intracranial aneurysms.</a> AJNR Am J Neuroradiol. 19, 1309-1314 (1998).</li><li>Bavinzski, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+bifurcation+aneurysm:+a+model+for+in+vivo+evaluation+of+endovascular+techniques.">Experimental bifurcation aneurysm: a model for in vivo evaluation of endovascular techniques.</a> Minim Invasive Neurosurg. 41, 129-132 (1998).</li><li>Spetzger, U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microsurgically+produced+bifurcation+aneurysms+in+a+rabbit+model+for+endovascular+coil+embolization.">Microsurgically produced bifurcation aneurysms in a rabbit model for endovascular coil embolization.</a> J Neurosurg. 85, 488-495 (1996).</li><li>Sherif, C., Marbacher, S., Erhardt, S., Fandino, J., J, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+microsurgical+creation+of+venous-pouch+arterial-bifurcation+aneurysms+in+rabbits.">Improved microsurgical creation of venous-pouch arterial-bifurcation aneurysms in rabbits.</a> AJNR. , .</li><li>Marbacher, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complex+Bi-Lobular,+Bi-Saccular,+and+Broad-Necked+Microsurgical+Aneurysms+Formation+in+the+Rabbit+Bifurcation+Model+for+the+Study+of+Upcoming+Endovascular+Techniques.">Complex Bi-Lobular, Bi-Saccular, and Broad-Necked Microsurgical Aneurysms Formation in the Rabbit Bifurcation Model for the Study of Upcoming Endovascular Techniques.</a> AJNR. , .</li><li>Sherif, C., Plenk, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+angiographic+and+histopathologic+evaluation+of+experimental+aneurysms.">Quantitative angiographic and histopathologic evaluation of experimental aneurysms.</a> AJNR. , .</li><li>Grunwald, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anticoagulation+regimes+and+their+influence+on+the+occlusion+rate+of+aneurysms:+an+experimental+study+in+rabbits.">Anticoagulation regimes and their influence on the occlusion rate of aneurysms: an experimental study in rabbits.</a> Neurosurg. 57, 1048-1054 (2005).</li><li>Forrest, M. D., O'Reilly, G. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Production+of+experimental+aneurysms+at+a+surgically+created+arterial+bifurcation.">Production of experimental aneurysms at a surgically created arterial bifurcation.</a> AJNR Am J Neuroradiol. 10, 400-402 (1989).</li></ol>

관심 없음 충돌 선언하지 않습니다.

저자는 배려 좋은 결과와 특허 동맥류를 달성하기 위해 다음과 같은 중요한 수술 핵심 단계를 권장합니다 <ol><li> 신경 작은 혈관의 파괴없이 쉽게 문합의 왼쪽 CCA의 긴 세그먼트의주의 해부. </li><li> 이전 문합의 창조에 매우 thrombogenic periadventitial 섬유 조직의 제거 조심하세요. </li><li> tensionless 문합의 창조. </li><li> 적을 봉합 : 이전 제안 절차에 비해 뒷면에서 시작 anastomsis 사이트의 제안 순서?...

<table border="1"><tbody><tr><th> 시약의 이름 </th><th> 회사 </th><th> 카탈로그 번호 </th><th> 댓글 </th></tr><tr><td> Vicryl4 - 0 polyfilament restorable 봉합 </td><td> Ethicon 부동산 </td><td> J386H </td><td></td></tr><tr><td> Ethilon 10-0 monofil 비 resorbable 봉합 </td><td> Ethicon 부동산 </td><td> 2814 </td><td> 테이퍼 포인트 바늘 </td></tr><tr><td> Evicel Bioglue </td><td> Ethicon Biosurgery 주식 회사 </td><td> 3901 </td><td></td></tr><tr><td> 펜타 닐 피부 패치 12.5 μg / H </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> 헤파린 </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> 0.9 %의 살린 </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> 4퍼센트 Papaverin HCL </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> 네오 마이신 황산 5 MG / ML </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> Ketamin 50mg/ml </td><td></td><td></td><td> 모든 genericon </td></tr><tr><td> Xylazine 20mg/ml </td><td></td><td></td><td> 모든 genericon </td></tr></tbody></table>

microsurgical venous pouch arterial-bifurcation aneurysms in the rabbit model: technical aspects

파열 인간의 대뇌 동맥류에 대한 endovascular embolization는 동맥류의 클리핑에 상응하는 대안이되고있다. 1 단, 큰 임상 시험은 동맥 recanalization 및 지연 동맥류 파열의 허용되지 않는 높은 속도로 실망 장기 결과를 표시합니다. 2는 이러한 문제를 극복하기 위해 동물 실험 연구 더 나은 endovascular 장치의 개발에 중요한 있습니다. 3-5 쥐, 토끼, 개부대 및 돼지 여러 동물 모델을 사용할 수 있습니다. 다른 동물 모델 6-8 비교 hemodynamics 및 응집 시스템의 comparability 및 비용 효율성에 관한 토끼 모델의 우수성을 보여주었다. 9-11 토끼의 정맥 주머니 동맥 분기점 모델은 모두 공통 경동맥 동맥 (CCA)의 인위적으로 만들어진 사실이 분기점에 봉합 정맥 주머니에 의해 형성됩니다. 이 모델의 주요 장점은 사실 bifurcational hemodynamics 있습니다. 12 주요 단점은 sofar 높은 microsurgical 기술적 요구 사항과 높은 병적 상태와 최대 50 %의 사망률 속도입니다. 13 이러한 제한은 최근이 동맥류 모델의 적은 자주 사용하는 결과가 년. 이러한 단점이 개선된 수술 절차를 극복하고 수정할 수있는 페리와 수술 analgetic 관리 anticoagulation. 14-16 우리 기술은 동맥 분기 동맥류의 microsurgical 생성이 최적화된 기술을 보여주는이 논문에보고했다.

Watch this Scientific Journal Video about Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects at JoVE.com

Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects

분기점 인간의 대뇌 동맥류을 흉내낸 동맥 분기 동맥류의 microsurgical 생성을위한 최적화된 기술이 설명되어 있습니다. 정맥 주머니는 모두 공통 경동맥 동맥의 인위적으로 만들어진 사실이 분기로 봉합니다. 촉진 microsurgical 기법과 적극적인 수술 anticoagulation와 진통제는 최소화 병적 속도와 높은 동맥 명백 속도로 이어집니다.

토끼 모델에서 Microsurgical 정맥 주머니 아티 - 분기점의 동맥류 : 기술적인 관점

For ruptured human cerebral aneurysms endovascular embolization has become an equivalent alternative to aneurysm clipping.1 However, large clinical trials ...

microsurgical-venous-pouch-arterial-bifurcation-aneurysms-rabbit

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13.9K 조회수.  Hospital Rudolfstiftung. 분기점 인간의 대뇌 동맥류을 흉내낸 동맥 분기 동맥류의 microsurgical 생성을위한 최적화된 기술이 설명되어 있습니다. 정맥 주머니는 모두 공통 경동맥 동맥의 인위적으로 만들어진 사실이 분기로 봉합니다. 촉진 microsurgical 기법과 적극적인 수술 anticoagulation와 진통제는 최소화 병적 속도와 높은 동맥 명백 속도로 이어집니다.

비디오: Microsurgical Venous Pouch Arterial-Bifurcation Aneurysms in the Rabbit Model: Technical Aspects

Technical Aspects of the Mouse Aortocaval Fistula

Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta and inferior vena cava (IVC) are exposed. The proximal infrarenal aorta and the distal aorta are dissected for clamp placement and needle puncture, respectively. Special attention is paid to avoid dissection between the aorta and the IVC. After clamping the aorta, a 25 G needle is used to puncture both walls of the aorta into the IVC. The surrounding connective tissue is used for hemostatic compression. Successful creation of the AVF will show pulsatile arterial blood flow in the IVC. Further confirmation of successful AVF can be achieved by post-operative Doppler ultrasound.

The goal is to produce an arteriovenous fistula that is simple and reproducible. This method does not use sutures or glue adhesive. Therefore the samples can be used with the least amount of foreign materials for analysis.

마우스 Aortocaval 누관의 기술적 측면

Technical aspects of creating an arteriovenous fistula in the mouse are discussed. Under general anesthesia, an abdominal incision is made, and the aorta ...

연구

의학

The Helsinki Rat Microsurgical Sidewall Aneurysm Model

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced into clinical practice. Furthermore, experimental models are needed to elucidate the complex aneurysm biology leading to rupture of saccular aneurysms.
Several different kinds of experimental models for saccular aneurysms have been established in different species. Many of them, however, require special skills, expensive equipment, or special environments, which limits their widespread use. A simple, robust, and inexpensive experimental model is needed as a standardized tool that can be used in a standardized manner in various institutions.
The microsurgical rat abdominal aortic sidewall aneurysm model combines the possibility to study both novel endovascular treatment strategies and the molecular basis of aneurysm biology in a standardized and inexpensive manner. Standardized grafts by means of shape, size, and geometry are harvested from a donor rat&#39;s descending thoracic aorta and then transplanted to a syngenic recipient rat. The aneurysms are sutured end-to-side with continuous or interrupted 9-0 nylon sutures to the infrarenal abdominal aorta.
We present step-by-step procedural instructions, information on necessary equipment, and discuss important anatomical and surgical details for successful microsurgical creation of an abdominal aortic sidewall aneurysm in the rat.

Microsurgical sidewall aneurysms in rats are created by end-to-side anastomosis of an aortic graft to the abdominal aorta. We present step-by-step instructions and discuss anatomical and surgical details for successful experimental saccular aneurysm creation.

헬싱키 쥐 미세 측벽 동맥류 모델

Experimental saccular aneurysm models are necessary for testing novel surgical and endovascular treatment options and devices before they are introduced ...

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and controllable animal models that simulate both conditions are presently uncommon. Therefore, new models are needed in order to mimic human pathophysiological conditions resulting from SAH.
This report describes the technical nuances of a rabbit blood-shunt SAH model that enables control of intracerebral pressure (ICP). An extracorporeal shunt is placed between the arterial system and the subarachnoid space, which enables examiner-independent SAH in a closed cranium. Step-by-step procedural instructions and necessary equipment are described, as well as technical considerations to produce the model with minimal mortality and morbidity. Important details required for successful surgical creation of this robust, simple and consistent ICP-controlled SAH rabbit model are described.

The experimental intracranial pressure-controlled blood shunt subarachnoid hemorrhage (SAH) model in the rabbit combines the standard procedures &#8212; subclavian artery cannulation and transcutaneous cisterna magna puncture, which enables close mimicking of human pathophysiological conditions after SAH. We present step-by-step instructions and discuss key surgical points for successful experimental SAH creation.

지주막 하 출혈의 급성과 늦은 후유증의 연구에 대한 토끼 피 션트 모델 : 기술적 측면

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and ...

A Murine Model of Arterial Restenosis: Technical Aspects of Femoral Wire Injury

Cardiovascular disease caused by atherosclerosis is the leading cause of death in the developed world. Narrowing of the vessel lumen, due to atherosclerotic plaque development or the rupturing of established plaques, interrupts normal blood flow leading to various morbidities such as myocardial infarction and stroke. In the clinic endovascular procedures such as angioplasty are commonly performed to reopen the lumen. However, these treatments inevitably damage the vessel wall as well as the vascular endothelium, triggering an excessive healing response and the development of a neointimal plaque that extends into the lumen causing vessel restenosis (re-narrowing). Restenosis remains a major cause of failure of endovascular treatments for atherosclerosis. Thus, preclinical animal models of restenosis are vitally important for investigating the pathophysiological mechanisms as well as translational approaches to vascular interventions. Among several murine experimental models, femoral artery wire injury is widely accepted as the most suitable for studies of post-angioplasty restenosis because it closely resembles the angioplasty procedure that injures both endothelium and vessel wall. However, many researchers have difficulty utilizing this model due to its high degree of technical difficulty. This is primarily because a metal wire needs to be inserted into the femoral artery, which is approximately three times thinner than the wire, to generate sufficient injury to induce prominent neointima. Here, we describe the essential surgical details to effectively overcome the major technical difficulties of this model. By following the presented procedures, performing the mouse femoral artery wire injury becomes easier. Once familiarized, the whole procedure can be completed within 20 min.

The mouse femoral artery wire injury model of restenosis is technically challenging. In this protocol we show the key technical details essential for successfully performing wire injury to induce consistent&#160;neointima for studies of restenosis.

동맥 재 협착의 쥐 모델 : 대퇴 와이어 부상의 기술적 측면

Cardiovascular disease caused by atherosclerosis is the leading cause of death in the developed world. Narrowing of the vessel lumen, due to ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and drugs. It is widely used in many studies on the physiological functions of vascular smooth muscle, the pathogenesis of vascular diseases such as hypertension, and the development of smooth muscle relaxant drugs. The mouse is a widely used model animal with a large pool of disease models and genetically modified strains. We introduced this method to measure the isometric contraction of mouse mesenteric artery in detail. A 1.4-mm segment of mouse mesenteric resistance artery was isolated and mounted on a myograph chamber by passing two steel wires through its lumen. After equilibration and normalization steps, the vessel segment was potentiated by a high-K+ solution twice prior to the contraction assay. As an example of the application of this method in drug development, we measured the relaxant effect of a novel natural substance, neoliensinine, isolated from a Chinese herb, embryos of the lotus seed (Nelumbo nucifera Gaertn.) on mouse mesenteric arteries. The vessel segments mounted on the myograph chamber were stimulated with a high-K+ solution. When the force tension reached a stable sustained phase, cumulative doses of neoliensinine were added to the chamber. We found that neoliensinine had a dose-dependent relaxant effect on smooth muscle contraction, thus suggesting that it bears potential activity against hypertension. In addition, as the vessel segment can survive at least 4 hours after mounting and maintain contractility induced by the high-K+ solution for many times, we suggest that the wire myograph system may be used for the time-consuming process of drug screening.

The wire myograph technique is used to investigate vascular smooth muscle functions and screen new drugs. We report a detailed protocol for measuring the isometric contractility of the mouse mesenteric artery and for screening new relaxants of vascular smooth muscle.

Myography 와이어를 사용 하 여 마우스 Mesenteric 동맥의 isometric 수축 측정

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...

Transthoracic Echocardiographic Examination in the Rabbit Model

Large animal models such as the rabbit are valuable for translational preclinical research. Rabbits have a similar cardiac electrophysiology compared to that of humans and that of other large animal models such as dogs and pigs. However, the rabbit model has the additional advantage of lower maintenance costs compared to other large animal models. The longitudinal evaluation of cardiac function using echocardiography, when appropriately implemented, is a useful methodology for preclinical assessment of novel therapies for heart failure with reduced ejection fraction (e.g. cardiac regeneration). The correct use of this non-invasive tool requires the implementation of a standardized examination protocol following international guidelines. Here we describe, step by step, a detailed protocol supervised by veterinary cardiologists for performing echocardiography in the rabbit model, and demonstrate how to correctly obtain the different echocardiographic views and imaging planes, as well as the different imaging modes available in a clinical echocardiography system routinely used in human and veterinary patients.

Here we describe, step by step, a detailed protocol for performing echocardiography in the rabbit model. We show how to correctly obtain the different echocardiographic views and imaging planes, as well as the different imaging modes available in a clinical echocardiography system routinely used in human and veterinary patients.

토끼 모델의 흉부 심초음파 검사

Large animal models such as the rabbit are valuable for translational preclinical research. Rabbits have a similar cardiac electrophysiology compared to ...

A Rabbit Venous Interposition Model Mimicking Revascularization Surgery using Vein Grafts to Assess Intimal Hyperplasia under Arterial Blood Pressure

Although vein grafts have been commonly used as autologous grafts in revascularization surgeries for ischemic diseases, the long-term patency remains poor because of the acceleration of intimal hyperplasia due to the exposure to arterial blood pressure. The present protocol is designed for the establishment of experimental venous intimal hyperplasia by interposing rabbit jugular veins to the ipsilateral carotid arteries. The protocol does not require surgical procedures deep in the body trunk and the extent of the incision is limited, which is less invasive for the animals, allowing long-term observation after implantation. This simple procedure enables researchers to investigate strategies to attenuate the progression of intimal hyperplasia of the implanted vein grafts. Using this protocol, we reported the effects transduction of microRNA-145 (miR-145), which is known to control the phenotype of vascular smooth muscle cells (VSMCs) from the proliferative to the contractile state, into harvested vein grafts. We confirmed the attenuation of intimal hyperplasia of vein grafts by transducing miR-145 before implantation surgery through the phenotype change of the VSMCs. Here we report a less invasive experimental platform to investigate the strategies that can be used to attenuate intimal hyperplasia of vein grafts in revascularization surgeries.

The present protocol aims to experimentally create venous intimal hyperplasia by subjecting veins to arterial blood pressure for developing strategies to attenuate venous intimal hyperplasia following revascularization surgery using vein grafts.

정맥 이식편을 사용하여 동맥 혈압하에서 인티맥 증식을 평가하는 재혈관 외과를 모방한 토끼 정맥 중개 모델

Although vein grafts have been commonly used as autologous grafts in revascularization surgeries for ischemic diseases, the long-term patency remains poor ...

Creation of Two Saccular Elastase-Digested Aneurysms with Different Hemodynamics in One Rabbit

Preclinical animal models with hemodynamic, morphologic, and histologic characteristics close to human intracranial aneurysms play a key role in the understanding of the pathophysiological processes and the development and testing of new therapeutic strategies. This study aims to describe a new rabbit aneurysm model that allows the creation of two elastase-digested saccular aneurysms with different hemodynamic conditions within the same animal.
Five female New Zealand white rabbits with a mean weight of 4.0 (&#177; 0.3) kg and mean age of 25 (&#177;5) weeks underwent microsurgical stump and bifurcation aneurysm creation. One aneurysm (stump) was created by right common carotid artery (CCA) exposure at its origin at the brachiocephalic trunk. A temporary clip was applied at the CCA origin and another, 2 cm above. This segment was treated with a local injection of 100 U of elastase for 20 min. A second aneurysm (bifurcation) was created by suturing an elastase-treated arterial pouch into the end-to-side anastomosis of the right CCA to left CCA. Patency was controlled by fluorescence angiography immediately after creation.
The average duration of surgery was 221 min. The creation of two aneurysms in the same animal was successful in all rabbits without complication. All aneurysms were patent immediately after surgery except for one bifurcation aneurysm, which showed an extreme tissue reaction due to elastase incubation and an immediate intraluminal thrombosis. No mortality was observed during surgery and up to one-month follow-up. Morbidity was limited to a transient vestibular syndrome (one rabbit), which recovered spontaneously within one day.
Demonstrated here for the first time is the feasibility of creating a two-aneurysm rabbit model with stump and bifurcation hemodynamic characteristics and highly degenerated wall conditions. This model allows the study of the natural course and potential treatment strategies on the basis of aneurysm biology under different flow conditions.

This protocol describes the steps for the creation of a rabbit model with two elastase-digested aneurysms with different hemodynamics (stump and bifurcation constellation). This allows the testing of novel endovascular devices in aneurysms with different angioarchitecture and hemodynamic conditions within a single animal.

한 토끼에서 서로 다른 혈역학을 가진 두 개의 천골 엘라스타제 소화 동맥류 생성

Preclinical animal models with hemodynamic, morphologic, and histologic characteristics close to human intracranial aneurysms play a key role in the ...

Isolated Lung Perfusion System in the Rabbit Model

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and macroscopically. This technique is useful in the characterization of pulmonary physiology and pathology by measuring metabolic activities and respiratory functions, including interactions between circulatory substances and the effects of inhaled or perfused substances, as in drug testing. While in vitro methods involve the slicing and culturing of tissues, the isolated ex vivo lung perfusion system allows to work with a complete functional organ making possible the study of a continuous physiological function while recreating ventilation and perfusion. However, it should be noted that the effects of the absence of central innervation and lymphatic drainage still have to be fully assessed. This protocol aims to describe the assembly of the isolated lung apparatus, followed by the surgical extraction and cannulation of lungs and heart from experimental lab animals, as well as to display the perfusion technique and signal processing of data. The average viability of the isolated lung ranges between 5-8 h; during this period, the pulmonary capillary permeability increases, causing edema and lung injury. The functionality of preserved pulmonary tissue is measured by the capillary filtration coefficient (Kfc), used to determine the extent of pulmonary edema through time.

The isolated rabbit lung preparation is a gold standard tool in pulmonary research. This publication aims to describe the technique as developed for the study of physiological and pathological mechanisms involved in airway reactivity, lung preservation, and preclinical research in lung transplantation and pulmonary edema.

토끼 모델의 절연 폐 관류 시스템

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and ...

A Rabbit Aortic Valve Stenosis Model Induced by Direct Balloon Injury

Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of access to reliable sources of diseased human aortic valves. Among the various animal models, AVS rabbit models are one of the most commonly used in large animal studies. However, traditional AVS rabbit models require a long-term period of dietary supplementation and genetic manipulation to induce significant stenosis in the aortic valve, limiting their use in experimental studies. To address these limitations, a new AVS rabbit model is proposed, in which stenosis is induced by a direct balloon injury to the aortic valve. The present protocol describes a successful technique for inducing AVS in New Zealand white (NZW) rabbits, with step-by-step procedures for the preparation, the surgical procedure, and the post-operative care. This simple and reproducible model offers a promising approach for studying the initiation and progression of AVS and provides a valuable tool for investigating the underlying pathological mechanisms of the disease.

An appropriate animal model is needed to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) and to evaluate the efficacy of therapeutic interventions. The present protocol describes a new procedure for developing the AVS rabbit model via a direct balloon injury in vivo.

직접적인 풍선 손상으로 인한 토끼 대동맥 판막 협착증 모델

Animal models are emerging as an important tool to understand the pathologic mechanisms underlying aortic valve stenosis (AVS) because of the lack of ...