로그인

JoVE Journal

Bioengineering

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments

11.6K Views

11:15 min

February 16th, 2012

February 16th, 2012

11,586 Views

0:05

Title

1:39

Preparation of the Electrostatic Chamber

2:54

Neural Progenitor Cell Isolation

4:09

Application of the Electric Field to the Electrostatic Chamber

7:18

Preparation of the Organotypic Spinal Cord Slice

8:40

Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice

9:38

Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice

10:46

Conclusion

This protocol demonstrates methods used to establish 2D and 3D environments in custom-designed electrotactic chambers, which can track cells in vivo/ex vivo using time-lapse recording at the single cell level, in order to investigate galvanotaxis/electrotaxis and other cellular responses to direct current (DC) electric fields (EFs).

당사 웹 사이트에서는 사용자의 경험을 향상시키기 위해 쿠키를 사용합니다.

당사 웹 사이트를 계속 사용하거나 '계속'을 클릭하는 것은 당사 쿠키 수락에 동의하는 것을 의미합니다.