NIH HL-10342에 의해 지원.

1. 절차 준비 하나는 먼저 획득하고 다음 항목을 준비해야합니다 : <ol><li> 캐뉼라. 20-35g 마우스의 삽관을 위해, 우리는 긴 1 또는 1.5 인치, 20 게이지 IV 카테터를 (BD Insylte, 스파크, MD 또는 Jelco Optiva, Carlsbad, CA)을 사용합니다. 새로운 멸균 카테터는 각 마우스에 사용할 수 있지만, 카테터는 하루 아침에 70 %의 에탄올에 몸을 담글 수에 의해 살균 후 재사용 할 수 있습니다. 마우스의 인두 나 기관도는 멸균 장갑과 악기의 사용을 포함한 멸균 적절한 청결 절차이지만, 준수해야합니다. </li><li> fiberoptic 케이블. 우리 오빠 광학에서 0.5 mm 광학 케이블의 ≈ 70cm를 사용하지만, 길이가 중요하지 않습니다. 이 레이저와 길이로 케이블을 절단 한 후, 에지가 상대적으로 좌회전되기 때문에 섬유, 그 가장자리 부드럽게이 있는지 확인하는 것이 중요합니다, 그것은 tracheal 벽 피어스에 많은 노력을하지 않습니다. 그러나, 전s은 (는) 매우 간단 1,000 그릿 멀리 종이를 (의 비디오 및 그림 1의 데모를 볼 터치 끝의 가장자리에 끝에서 2cm에 대한 섬유를 개최 한 후 몇 초 동안 작은 원을 만들어이 가장자리를 부드럽게하기 맥도널드, 외. 10). 다른 쪽 끝은 고무 마개를 통해 삽입됩니다. 이것은 가장 쉽게 첫번째 다음 바늘을 인출, 마개를 통해 18 게이지 바늘을 밀어 바늘 구멍을 통해 광섬유를 삽입하여 수행됩니다. 고무 마개 (예 NCL-150, Volpi 미국, 또는 기타 나 광원, 심지어 미만 150w) 150 와트 할로겐 라이트 소스에 연결되어 있습니다. 그것은 뜨거운 광원에 너무 가까이 위치하고 때 일반 고무 또는 코르크 굽기 수 있으므로, 실리콘 고무 (또는 다른 열 저항 소재)로 만든 마개를 사용하도록하십시오하는 것이 중요합니다. </li></ol> 2. 삽관 법을 수행 <ol><li> 도 1 및도 2를 참조하십시오. 광섬유 케이블 t를 삽입hrough 실리콘 고무 튜브의 짧은 부분 (≈ 0.8 mm ID X 4mm OD, 콜 - 팔머, EW-96410-13). 여전히 광섬유 케이블을 조정할 수 있도록하면서 매우 긴밀한이 고무 튜브를 묶어주세요. 캐뉼라의 Luer 끝에 석판 실리콘 튜브를 삽입하면 삽입 관 내부의 광섬유 케이블의 위치를​​ 수정합니다. 이 캐뉼라 끝의 앞에 캐뉼라 ≈ 4mm 통해 연장하도록 fiberoptic 케이블의 위치를​​ 조정합니다. </li><li> 의 상단 incisors (그림 3)에 의해 중지 수직 지원에 anesthetized 마우스를 놓습니다. 대부분의 조사는 자신이 직면하고있는 마우스의 복부 측면과 최고의 시각화를 찾으십시오. 아주 부드럽게 혀를 뽑아서 엄지와 집게 손가락으로 누르고 있습니다. 가운데 손가락은 목 및 플라스틱 지원 사이에 위치합니다. 집게 손가락과 엄지 손가락으로 혀 견인가 입을 열 및 삽관 경로를 바르게하는 데 사용됩니다, 머리의 각도는 목 뒤의 가운데 손가락으로 조정됩니다그림 3에 표시된. </li><li> 가벼운 소스와 소개 자로서 광섬유 케이블을 사용하여 시각화 성대를 통해 밀어. 코드가 표시되지 않으면 부드럽게 지원으로 가운데 손가락을 사용하여 혀에 더 당긴다. 삽입 할 때 더 캐뉼라 ≈ 5mm을 부활 시켰습니다. 그런 다음 매우 조심하는 것은, 캐뉼라를 이동 광섬유 케이블을 인출 할 수 없습니다. 마우스를 누워 캐뉼라를 확보 테이프 조각을하고 Plasticine (모델링 점토)의 작품에 캐뉼라 허브를 지원 그림 4에 표시. </li><li> 가 솔로 작업이기 때문에 3 단계의 절차는 쉽게 가르쳐거나, 입증 할 수 없습니다. 그러나, 혀와 머리 뒤의 지원에 견인의 미묘한 조정이 거의이 시도하는 모든 곧 성대를 시각화하기 위해 마우스를 위치 할 수있는 올바른 방법을 찾으십시오. </li></ol>

<ol>
	<li>Brown, R. H., Walters, D. M., Greenberg, R. S., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+of+endotracheal+intubation+and+pulmonary+functional+assessment+for+repeated+studies+in+mice.">A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice.</a> J. Appl. Physiol. 87, 2362-2365 (1999).</li><li>Walters, D. M., Wills-Karp, M., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+cellular+profile+and+lung+function+with+repeated+bronchoalveolar+lavage+in+individual+mice.">Assessment of cellular profile and lung function with repeated bronchoalveolar lavage in individual mice.</a> Physiol. Genomics. 2, 29-36 (2000).</li><li>Rivera, B., Miller, S., Brown, E., Price, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+method+for+endotracheal+intubation+of+mice+and+rats+used+in+imaging+studies.">A novel method for endotracheal intubation of mice and rats used in imaging studies.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 44, 52-55 (2005).</li><li>Hamacher, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+wire+guide-based+orotracheal+mouse+intubation:+description,+evaluation+and+comparison+with+transillumination.">Microscopic wire guide-based orotracheal mouse intubation: description, evaluation and comparison with transillumination.</a> Laboratory Animals. 42, 222-230 (2008).</li><li>Spoelstra, E. N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+and+simple+method+for+endotracheal+intubation+of+mice.">A novel and simple method for endotracheal intubation of mice.</a> Laboratory Animals. 41, 128-135 (2007).</li><li>Zhao, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+technique+for+retrograde+intubation+in+mice.">A technique for retrograde intubation in mice.</a> Lab Animal. 35, 39-42 (2006).</li><li>Vergari, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+new+method+of+orotracheal+intubation+in+mice.">A new method of orotracheal intubation in mice.</a> European Review for Medical and Pharmacological Sciences. 8, 103-106 (2004).</li><li>Vergari, A., Polito, A., Musumeci, M., Palazzesi, S., Marano, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Video-assisted+orotracheal+intubation+in+mice.">Video-assisted orotracheal intubation in mice.</a> Laboratory Animals. 37, 204-206 (2003).</li><li>Hastings, R. H., Summers-Torres, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+Laryngoscopy+in+Mice.">Direct Laryngoscopy in Mice.</a> Contemporary Topics in Laboratory Animal Science / American Association for Laboratory Animal Science. 38, 33-35 (1999).</li><li>MacDonald, K. D., Chang, H. Y., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+improved+simple+method+of+mouse+lung+intubation.">An improved simple method of mouse lung intubation.</a> J. Appl. Physiol. 106, 984-987 (2009).</li><li>Ewart, S. L., Levitt, R. C., Mitzner, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Respiratory+system+mechanics+in+mice+measured+by+end-inflation+occlusion.">Respiratory system mechanics in mice measured by end-inflation occlusion.</a> Journal of Applied Physiology. 79, 560-566 (1995).</li><li>MacDonald, K. D., McKenzie, K. R., Mitzner, W., Zeitlin, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lung+Mechanics+in+Heterozygous+CF+Mice+after+Repeated+LPS+Dosing.">Lung Mechanics in Heterozygous CF Mice after Repeated LPS Dosing.</a> Am. J. Respir. Crit. Care Med. 175 (4), A930 (2007).</li></ol>

저자는 모두 공개 할 관심의 충돌이 없습니다.

여기에 설명 된 절차는 몇 가지 장점이 있습니다. 먼저 장치 간단하고 상대적으로 저렴 .. 장치의 제조는 특별한 도구 나 비싼 장비가 필요하지 않습니다. 카테터의 사용은 또한 광원이 하나가 소개 자의 tracheal 개방에 접근로 tracheal 개방의 시력을 잃게 없다는 의미입니다 소개합니다. 0.5 mm의의 소개 자 사용은 큰 캐뉼라의 초기 삽입으로 발생할 수있는 충격을 최소화하는 역할을한다. 우리는 유?...

1999 년 브라운 외이 있습니다. 마우스 폐 1 삽관하는 방법을 설명하는 논문을 발표했다. 이러한 기술은 종 방향 연구 2 개인 마우스에 반복 폐 기능 또는 bronchoalveolar 세척을하고있어서 상당한 유틸리티가 있습니다. 그 원래의 종이 있기 때문에, 마우스 삽관 3-9에 다른 방법을 설명했습니다 몇 가지 다른 논문도했습니다. 이러한 방법의 모든 성공적으로 사용할 수 있지만, 그들은 일반적으로 상당한 훈련 또는 비용이 필요합니다. 이러한 삽관과 주요 문제 중 하나는 삽관 법의 캐뉼라 방식으로 기관 중 삽입 가까워 것입니다, 캐뉼라 자체가 빛과 따라서이 가야 할 곳의 시각화를 차단합니다. 따라서, 삽입은 가장 중요한 시간에 맹인이된다. 이 논문에서 우리는 간단하고 저렴한 비용으로함으로써 상대적으로 약간의 훈련 또는과 성공적인 삽관을 확보,이 시각화 문제를 제거하는 방법을 보여줍니다경험.

<table border="1"><tbody><tr><td> 자료 </td><td> 회사 </td><td> # 카탈로그를 </td><td> 논평 </td></tr><tr><td> 삽관 법의 캐뉼라 </td><td> BD Insylte, 스파크, MD 또는 Jelco Optiva, Carlsbad, CA </td><td></td><td> 1 인치 길이, 20 게이지 정맥 주사 (IV) 카테터 </td></tr><tr><td> 광섬유 케이블 </td><td> 에드먼드 광학, 배 링턴, NJ </td><td> # NT02-542 </td><td> 약 2 피트 0.5-mm 광섬유의 길이 (통신 학년 플라스틱 섬유). 기관에 삽입되는 섬유 끝의 가장자리가 부드럽게 1000 그릿 모리 용지에 몇 초 동안 팁을 드래그 ≈ 2cm 끝에서 섬유를 개최하고 작은 원을 만들어 둥글게해야합니다. </td></tr><tr><td> 광원 </td><td> Volpi </td><td> NCL-150 </td><td> 우리는 훨씬 낮은 와트와 150 W 할로겐 광원, 이와 동등한 광원을 사용하지만사용할 수 있습니다. </td></tr><tr><td> 실리콘 고무 마개 </td><td></td><td></td><td> # 1 실리콘 고무 마개는이 예제에 사용되는 광원에 맞는. 다른 광원는 다른 크기를 필요로 할 수 있습니다. </td></tr><tr><td> 작은 실리콘 고무 튜브 </td><td> 콜 - 팔머 </td><td> EW-96410-13 </td><td> 실리콘 고무 튜브의 ≈ 1.5 cm 피스 (0.8 내부 직경 4mm 외경) </td></tr><tr><td> 각진 지원 스탠드 </td><td></td><td></td><td> 우리는 플렉시 글라스에서 constucted되어 있지만, 스레드 또는 와이어 거의 수직 각도로 마우스를 잡아 부착 할 수 있습니다에 대한 어떤 자료를 사용할 수 있습니다. </td></tr></tbody></table>

a simple method of mouse lung intubation

폐 기능 측정을위한 쥐를 intubate하는 간단한 절차 제한된 숫자 나 값 비싼 동물과 세로 방향의 연구에 몇 가지 장점을 얻을 수 있습니다. 이 더 정기적으로 수행되지 않는 이유 중 하나는이 그것을 달성 할 수있는 방법을 설명하는 여러 출판 연구 임에도 불구하고, 상대적으로 어려운 것입니다. 이 논문에서 우리는이 삽관과 관련된 주요 장애물 중 하나 삽관 법의 전체 시간 동안기도를 시각화의를 제거하는 절차를 보여줍니다. 방법은 마우스 기관에 삽관 법의 캐뉼라를 직접 할 수있는 소개 자 역할을하는 0.5 mm fiberoptic 광원을 사용합니다. 우리는 적어도 몇 주 시간 과정을 통해 개인의 쥐 폐 기계를 측정하는이 절차를 사용하는 것이 가능하다는 것을 보여줍니다. 기술은 비교적 적은 비용과 전문 지식을 설정할 수 있습니다, 그리고 그것은 정기적으로 상대적으로 약간의 훈련을 수행 할 수 있습니다. 이 MAK해야합니다이 가능한 연구실은 정기적으로함으로써 이에 필요한 마우스의 수를 최소화하고 자신의 제어로 각 마우스를 사용하여 통계 전력을 증가, 개인 마우스의 종 방향 연구를 수 있도록,이 삽관 법을 수행 할 수 있도록 전자.

방법의 평가, 우리는 27.7의 평균 중량 (± SEM) ± 0.40 g없이 네 20주 세의 남자 BALB / C 마우스를 사용했습니다. 이전 11 설명 된대로 폐 저항이 시스템을 사용하여 측정 된 곳들은, 다섯 경기를 연속으로 주를 공부했다. 각 마우스 IP 주입을 통해 케타민 (100 μg / g BW) 및 염분의 xylazine (15 μg / g BW)와 anesthetized했습니다. 위에서 설명한대로 그들은 그 다음 intubated되었습니다. 캐뉼라가 기관이 아닌 ?...

Watch this Scientific Journal Video about A Simple Method of Mouse Lung Intubation at JoVE.com

A Simple Method of Mouse Lung Intubation

본 논문은 폐 기능 측정 또는 쥐가 복구 나중에 시간에 공부를 할 수 있습니다 폐 기지에 대한 쥐를 intubating의 striaghforward하고 효율적인 방법을 설명합니다. 절차는 직접 기관을 비춰 저렴한 fiberoptic 광원을 포함한다.

마우스 폐 삽관 법의 간단한 방법

A  simple procedure to intubate mice for pulmonary function measurements would  have several advantages in longitudinal studies with limited numbers or  ...

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Medicine

50.5K 조회수.  Johns Hopkins Bloomberg School of Public Health. 본 논문은 폐 기능 측정 또는 쥐가 복구 나중에 시간에 공부를 할 수 있습니다 폐 기지에 대한 쥐를 intubating의 striaghforward하고 효율적인 방법을 설명합니다. 절차는 직접 기관을 비춰 저렴한 fiberoptic 광원을 포함한다.

비디오: A Simple Method of Mouse Lung Intubation

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified version of carotid partial ligation methods [1,2] to show that it acutely induces low and oscillatory flow conditions, two key characteristics of disturbed flow, in the mouse common carotid artery. Using this model, we have provided direct evidence that disturbed flow indeed leads to rapid and robust atherosclerosis development in Apolipoprotein E knockout mouse [3]. We also developed a method of endothelial RNA preparation with high purity from the mouse carotid intima [3]. Using this mouse model and method, we found that partial ligation causes endothelial dysfunction in a week, followed by robust and rapid atheroma formation in two weeks in a hyperlipidemic mouse model along with features of complex lesion formation such as intraplaque neovascularization by four weeks. This rapid in vivo model and the endothelial RNA preparation method could be used to determine molecular mechanisms underlying flow-dependent regulation of vascular biology and diseases. Also, it could be used to test various therapeutic interventions targeting endothelial dysfunction and atherosclerosis in considerably reduced study duration.

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

부분 결합하여 마우스 경동맥 동맥에있는 방해 흐름 유발 죽상 경화증과 경동맥 내피로부터 RNA 분리의 간단한 방법의 모델

Despite the well-known close association, direct evidence linking disturbed flow to atherogenesis has been lacking. We have recently used a modified ...

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의학

Guidelines for Elective Pediatric Fiberoptic Intubation

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small babies will desaturate very quickly if ventilation is interrupted mainly to high metabolic rate. We describe guidelines to perform a safe fiberoptic intubation while maintaining spontaneous breathing throughout the procedure. Steps requiring the use of propofol pump, fentanyl, glycopyrrolate, red rubber catheter, metal insuflation hook, afrin, lubricant and lidocaine spray are shown.

We describe guidelines to perform a safe and efficient elective fiberoptic intubation in pediatric patients while maintaining spontaneous ventilation.

선택 소아 Fiberoptic 삽관법에 대한 지침

Fiberoptic intubation in pediatric patients is often required especially in difficult airways of syndromic patients i.e. Pierre Robin Syndrome. Small ...

A Simple Guide Screw Method for Intracranial Xenograft Studies in Mice

The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma (glioma) and medulloblastoma. The widely used stereotactic approach only allows for the injection of a single animal at a time, is labor intensive and requires highly specialized equipment. The guide screw method, initially developed by Lal et al.,1 was developed to eliminate cumbersome stereotactic procedures. We now describe a modified guide screw approach that is rapid and exceptionally safe; both of which are critical ethical considerations. Notably, our procedure now incorporates an infusion pump that allows up to 10 animals to be simultaneously injected with tumor cells.
To demonstrate the utility of this procedure, we established human U87MG glioma cells as intracranial xenografts in mice, which were then treated with AMG102; a fully human antibody directed to HGF/scatter factor currently undergoing clinical evaluation2-5. Systemic injection of AMG102 significantly prolonged the survival of all mice with intracranial U87MG xenografts and resulted in a number of complete cures. 
This study demonstrates that the guide screw method is an inexpensive, highly reproducible approach for establishing intracranial xenografts. Furthermore, it provides a relevant physiological model for validating novel therapeutic strategies for the treatment of brain cancers.

In order to evaluate novel therapeutic paradigms for the treatment of glioma, physiological relevant models are essential. We utilize an implantable guide screw procedure for establishment of intracranial xenograft models that is more rapid and safer than stereotactic approaches.

마우스에 Intracranial 이종 이식 연구에 대한 간단한 안내 스크류 방법

The grafting of human tumor cells into the brain of immunosuppressed mice is an established method for the study of brain cancers including glioblastoma ...

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. With the increasing availability of knockout and transgenic derivatives, together with a vast amount of genetic information, mice provide one of the best models to study the molecular mechanisms underlying the pathology and physiology of lung diseases. Inhalation, intranasal instillation, intratracheal instillation, and intratracheal intubation are the most widely used techniques by a number of investigators to administer materials of interest to mouse lungs. There are pros and cons for each technique depending on the goals of a study. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented. This technique was applied to administer bleomycin to mouse lungs as a model to study pulmonary fibrosis.

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular disease and for developing therapeutic intervention. Here a noninvasive intratracheal intubation method that can directly deliver exogenous materials to mouse lungs is presented.

비 침습적 기관 내 삽관 마우스 폐의 병리학 및 생리학을 연구하는

The use of a model that mimics the condition of lung diseases in humans is critical for studying the pathophysiology and/or etiology of a particular ...

Implantation of Fibrin Gel on Mouse Lung to Study Lung-specific Angiogenesis

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and repair damage in simple tissues in the orthopedic and periodontal fields. However, attempts to regenerate the structures and functions of more complex three-dimensional (3D) organs such as lungs have not been very successful because the biological processes of organ regeneration have not been well explored. It is becoming clear that angiogenesis, the formation of new blood vessels, plays key roles in organ regeneration. Newly formed vasculatures not only deliver oxygen, nutrients and various cell components that are required for organ regeneration but also provide instructive signals to the regenerating local tissues. Therefore, to successfully regenerate lungs in an adult, it is necessary to recapitulate the lung-specific microenvironments in which angiogenesis drives regeneration of local lung tissues. Although conventional in vivo angiogenesis assays, such as subcutaneous implantation of extracellular matrix (ECM)-rich hydrogels (e.g., fibrin or collagen gels or Matrigel - ECM protein mixture secreted by Engelbreth-Holm-Swarm mouse sarcoma cells), are extensively utilized to explore the general mechanisms of angiogenesis, lung-specific angiogenesis has not been well characterized because methods for orthotopic implantation of biomaterials in the lung have not been well established. The goal of this protocol is to introduce a unique method to implant fibrin gel on the lung surface of living adult mouse, allowing for the successful recapitulation of host lung-derived angiogenesis inside the gel. This approach enables researchers to explore the mechanisms by which the lung-specific microenvironment controls angiogenesis and alveolar regeneration in both normal and pathological conditions. Since implanted biomaterials release and supply physical and chemical signals to adjacent lung tissues, implantation of these biomaterials on diseased lung can potentially normalize the adjacent diseased tissues, enabling researchers to develop new therapeutic approaches for various types of lung diseases.

Recapitulation of the organ-specific microenvironment, which stimulates local angiogenesis, is indispensable for successful regeneration of damaged tissues. This report demonstrates a novel method to implant fibrin gels on the lung surface of living mouse in order to explore how the lung-specific microenvironment modulates angiogenesis and alveolar regeneration in adult mouse.

마우스 폐에 섬유소 젤의 주입은 폐 관련 신생 혈관 연구에

Recent significant advances in stem cell research and bioengineering techniques have made great progress in utilizing biomaterials to regenerate and ...

Intubation-mediated Intratracheal (IMIT) Instillation: A Noninvasive, Lung-specific Delivery System

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences between the murine and human respiratory systems, especially in their upper respiratory tracts (URT). In some models, these differences in the murine nasal cavity can have a significant impact on disease progression and presentation in the lower respiratory tract (LRT) when using intranasal instillation techniques, potentially limiting the usefulness of the mouse model to study these diseases. For these reasons, it would be advantageous to develop a technique to instill bacteria directly into the mouse lungs in order to study LRT disease in the absence of involvement of the URT. We have termed this lung specific delivery technique intubation-mediated intratracheal (IMIT) instillation. This noninvasive technique minimizes the potential for instillation into the bloodstream, which can occur during more invasive traditional surgical intratracheal infection approaches, and limits the possibility of incidental digestive tract delivery. IMIT is a two-step process in which mice are first intubated, with an intermediate step to ensure correct catheter placement into the trachea, followed by insertion of a blunt needle into the catheter to mediate direct delivery of bacteria into the lung. This approach facilitates a &#62;98% efficacy of delivery into the lungs with excellent distribution of reagent throughout the lung. Thus, IMIT represents a novel approach to study LRT disease and therapeutic delivery directly into the lung, improving upon the ability to use mice as surrogates to study human respiratory disease. Furthermore, the accuracy and reproducibility of this delivery system also makes it amenable to Good Laboratory Practice Standards (GLPS), as well as delivery of a wide range of reagents which require high efficiency delivery to the lung.

Intubation-mediated Intratracheal IMIT Instillation: A Noninvasive, Lung-specific Delivery System

Intubation-mediated intratracheal (IMIT) instillation of reagents is an excellent, noninvasive method for studying respiratory disease, as well as a method for instilling therapeutic reagents directly into the lung. It is a rapid and highly reproducible method which is suitable for preclinical testing.

삽관 매개 기관 내 (IMIT) 떨어 뜨림 : 비 침습적, 폐 별 전달 시스템

Respiratory disease studies typically involve the use of murine models as surrogate systems. However, there are significant physiologic differences ...

Mouse Pneumonectomy Model of Compensatory Lung Growth

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to disability or death. Injury-based animal models of lung repair and regeneration are limited by injury-specific responses making it difficult to differentiate changes related to the injury response and injury resolution from changes related to lung repair and lung regeneration. However, use of animal models to identify these repair and regeneration signaling pathways is critical to the development of new therapies aimed at improving pulmonary function following lung injury. The mouse pneumonectomy model utilizes compensatory lung growth to isolate those repair and regeneration signals in order to more clearly define mechanisms of alveolar re-septation. Here, we describe our technique for performing mouse pneumonectomy and sham pneumonectomy. This technique may be utilized in conjunction with lineage tracing or other transgenic mouse models to define molecular and cellular mechanism of lung repair and regeneration.

Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.

보상 성 폐 성장의 마우스 전폐 절제술 모델

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to ...

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. As this technology expands and improves, the ability to potentially evaluate and improve the quality of substandard lungs prior to transplant is a critical need. In order to more rigorously evaluate these approaches, a reproducible animal model needs to be established that would allow for testing of improved techniques and management of the donated lungs as well as to the lung-transplant recipient. In addition, an EVLP animal model of associated pathologies, e.g., ventilation induced lung injury (VILI), would provide a novel method to evaluate treatments for these pathologies. Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs. The goal is to provide the research community with key information and &#8220;pearls of wisdom&#8221;/techniques that arose from trial and error and are critical to establishing an EVLP system that is robust and reproducible.

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.

고립의 방법<em&gt; 전의 VIVO</em쥐 모델에서&gt; 폐 관류 : 쥐 EVLP 프로그램 개발에서 교훈을

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe the use of a simple device to cut and &#8216;roll&#8217; mouse intestines to rapidly prepare whole mount preparations of superior and uniform quality to that which can be achieved by hand. The device comprises a base that holds 4 stainless steel rods and a top, which acts a cutting guide. The rods are inserted into the lumen of the small intestine [divided into thirds] and the colon. The rods and samples are then placed over a piece of filter paper or card into the holding slots in the base of the device. The top of the device is then positioned and serves as a cutting guide. The two angled sections in the center of the top piece are used to guide a knife or scalpel and cut the intestines longitudinally on the top of the rods. Once the intestinal sections have been cut, the top is removed and the card,&#160;tissue and rods gently removed from the device and placed on the bench. The rods are then gently rolled sideways to flatten and stick the intestinal segments onto the underlying piece of filter paper or card. The final preparation can then be examined or fixed and stored for later analysis. The preparations are invaluable for the study of intestinal changes in normal or genetically modified mouse models. The preparations have been used for the study and quantification of the effects of inflammation (colitis), damage, pre-cancerous lesions (aberrant crypt foci (ACFs) and mucin depleted foci (MDFs)) and polyps or tumors.

The use of a simple device to cut and &#8216;roll&#8217; mouse intestines to rapidly prepare whole mount preparations is described.

간단한 장치는 빠르게 마우스 소장의 전체 마운트를 준비하는 방법

Preparing whole mounts of the mouse small intestine and colon for subsequent analysis or quantification can be time consuming and difficult. We describe ...

An Improved Method for Rapid Intubation of the Trachea in Mice

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity is a commonly used model to study both acute lung injury and fibrosis, and multiple methods have been developed for administering bleomycin (and other toxic agents) into the lungs. However, many of these approaches, such as transtracheal instillation, have inherent drawbacks, including the need for strong anesthetics and survival surgery. This paper reports a quick, reproducible method of intratracheal intubation that involves mild inhaled anesthesia, visualization of the trachea, and the use of a surrogate spirometer to confirm exposure. As a proof of concept, 8-12 week old C57BL/6 mice were administered either 2.0 U/kg of bleomycin or an equivalent volume of PBS, and both damage and fibrotic endpoints were measured post-exposure. This procedure allows researchers to treat a large cohort of mice in a relatively short period with little expense and minimal post-procedure care.

This article presents a rapid and simple method for administering bleomycin directly into the mouse trachea via intubation. Key advantages of this method are that it is highly reproducible, easy to master, and does not require specialized equipment or lengthy recovery times.

마우스의기도 신속 삽관 용 개선 된 방법

Despite some anatomical and physiological differences, mouse models continue to be an essential tool for studying human lung disease. Bleomycin toxicity ...