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Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

DOI :

10.3791/50633-v

July 22nd, 2013

July 22nd, 2013

20,779 Views

1Department of Pathology and Cell Biology, Columbia University, 2Herbert Irving Comprehensive Cancer Center, Columbia University

We describe the use of two ratiometric, genetically encoded biosensors, which are based on GFP, to monitor mitochondrial redox state and ATP levels at subcellular resolution in living yeast cells.

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Keywords Mitochondrial Redox State

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