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JoVE Journal

Medicine

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Collecting Saliva and Measuring Salivary Cortisol and Alpha-amylase in Frail Community Residing Older Adults via Family Caregivers

11.3K Views

08:25 min

December 18th, 2013

December 18th, 2013

11,276 Views

0:05

Title

1:10

Preparation of Saliva Collection Supplies

2:24

Saliva Collection Instruction

3:44

Provide Additional Instructions and Answer Questions

4:32

Saliva Handling and Processing and Salivary Cortisol Assay Protocol

6:44

Results: Collection of Salivary Biomeasures in the Community Setting

7:53

Conclusion

We demonstrate: (1) procedures for collection of salivary samples in cognitive impaired older adults by family caregivers in the home setting, (2) procedures for measuring stress activity via salivary cortisol and alpha amylase, and (3) representative profiles. Protocols that allow researchers to study stress-linked processes advance our understanding of biological sensitivity and susceptibility.

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