November 28th, 2014
•We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.
Related Videos
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
Steady-state, Pre-steady-state, and Single-turnover Kinetic Measurement for DNA Glycosylase Activity
Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Dynamic Electrochemical Measurement of Chloride Ions
A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction
Preparation and Reactivity of a Triphosphenium Bromide Salt: A Convenient and Stable Source of Phosphorus(I)
Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS
Microfluidic Preparation of Liquid Crystalline Elastomer Actuators
Synthesis of Esters Via a Greener Steglich Esterification in Acetonitrile
NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
Copyright © 2024 MyJoVE Corporation. 판권 소유