September 10th, 2015
•This article describes the detailed protocol and equipment necessary for dual optical mapping of transmembrane potential (Vm) and free intra-sarcoplasmic reticulum (SR) Ca2+ in the Langendorff-perfused rabbit heart. This method allows for direct observation and quantification of Vm and SR Ca2+ dynamics in the intact heart.
Related Videos
Proper Care and Cleaning of the Microscope
Optical Mapping of Langendorff-perfused Rat Hearts
Semi-automated Optical Heartbeat Analysis of Small Hearts
Preparation of embryos for Electron Microscopy of the Drosophila embryonic heart tube
Quantification of γH2AX Foci in Response to Ionising Radiation
Quantitation of γH2AX Foci in Tissue Samples
Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation
Clonogenic Assay: Adherent Cells
Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
High-resolution Optical Mapping of the Mouse Sino-atrial Node
Copyright © 2024 MyJoVE Corporation. 판권 소유