Flank Skin Harvest and Infiltrating Leukocyte Isolation
4:28
Results: Oxazolone (Ox) Challenge Results in Pronounced T Cell and Neutrophil Flank Skin Infiltration
6:17
Conclusion
필기록
The overall goal of this procedure is to isolate skin-infiltrating leukocytes using enzymatic digestion and density gradient separation. This method can be applied to the investigation of cellular and molecular mechanisms in many mouse models of s
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This protocol describes enzymatic digestion of mouse skin in nutrient-rich medium followed by gradient separation to isolate leukocytes. Cells thus derived can be used for diverse downstream applications. This is an effective, economical, and improved alternative to tissue dissociation machines and harsher trypsin and dispase-based tissue digestion protocols.