This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. 실버 나노 입자 합성 (15) <ol><li> 5 초 동안 100 ㎖의 초순수 (18.2 ΩU)와 소용돌이에 18 mg의 질산은을 녹인다. </li><li> 5 초 동안 1 ml의 물과 소용돌이에 27 mg의 구연산 나트륨 이수화 물을 녹인다. </li><li> 교반 막대를 함유하는 삼각 플라스크에 제조 된 질산은 용액을 모두 전송하고 자기 핫 플레이트 상 형틀을 넣어. ~ 350 ° C에서 700 rpm의 교반 속도로 격렬하게 교반하에 플라스크를 가열 (접시에 온도를 설정). </li><li> 끓는되면 바로 삼각 플라스크에 제조 된 구연산 나트륨 용액 모두를 추가하고, 용액의 Ag 된 NP의 형성을 나타내는 녹갈색을 변할 때까지 추가로 25 분 동안 비등 용액을 떠난다. </li><li> 핫 플레이트에서 플라스크를 제거하고 일정한 색상과 트란과 다른 자기 접시에 넣어 (가열하지 않음) 및 교반 O는 / N은 ​​실온에서 동일한 교반 속도에서 혼합물이 안정 상태에 도달 할 때까지sparency. 필요한 경우 제조 된 NP의 Ag의 흡광도를 결정하는 UV-힘 분광계를 사용한다. </li><li> 100ml의 초순수로 최종 혼합물을 희석. </li><li> 필요한 경우 제조사의 프로토콜에 따라의 Ag NP에의 크기를 측정하는 제타를 사용합니다. </li><li> 밀폐 용기에의 Ag 콜로이드를 전송하고, 알루미늄 호일로 광으로부터 보호한다. 필요한 경우 콜로이드 2 개월 동안 4-7 ℃에서 냉장고에 저장 될 수있다. </li></ol> SERS 액티브 필터 멤브레인 2. 제작 <ol><li> 50 mM의 NaCl 용액을 100 mL의 물에 2.92 g의 염화나트륨 (염화나트륨)을 녹인다. </li><li> 제조의 Ag NP에 1 ㎖에 5 mM의 NaCl 용액 1 ㎖를 첨가하고 20 rpm에서 10 분 동안 하향 경사 믹서 섞는다. 이 단계의 Ag 나노 클러스터로의 Ag NPS를 집계하는 것입니다. </li><li> 주사기에 부착 될 수있는 필터 홀더에 필터 막 (PVDF, 0.1㎛의 공극 크기)을 놓는다. 작은 기공 크기의 막 fo를이었다일치 신호의 Ag 나노 클러스터를 포착 및 생성에 큰 기공 크기 막 (즉, 0.22 μm의)보다 더 효과 싶게. </li><li> 로드 여과 주사기에 제조의 Ag 나노 클러스터 2 ㎖. 주사기 필터 홀더를 부착하여 수동 1 방울 / 초의 유속으로 막을 통과의 Ag 나노 클러스터의 전체 볼륨을 통과한다. 막 트랩의 Ag 나노 클러스터는 SERS 활성 여과막을 형성한다. </li><li> 필터 홀더에 필터 멤브레인을 분리. 멤브레인에 손상이 없도록 핀셋을 사용하여 외부 테두리에 멤브레인을 들고 때 특별한주의가 필요합니다. 유리 슬라이드에 약 3 분 장소 막의 건조 공기. </li><li> SERS 기판의 라만 검출 <ol><li> 5 mW의, 10X 현미경 대물 렌즈로 설정 (2)의 1 초간 노출 수 노출 시간의 레이저 출력과 파장 780 nm 레이저 라만 악기를 설정한다. 소프트웨어의 목적은 너무 따라 설정되어 있는지 확인합니다. &lt;/ 리&gt; <li> 라만 장비의 플랫폼 상 위에 막으로 유리 슬라이드에 놓고 막 표면에 초점 현미경을 사용한다. </li><li> 무작위로 막 표면에서 8-10 지점을 선택하고 기기는 순서대로 자동으로 수집합니다. 분석을위한 제조업체의 소프트웨어에서 열기 스펙트럼 데이터. </li></ol></li></ol> SERS 액티브 필터 시스템 3. 응용 화학 오염 물질을 감지하는 <ol><li> 10 PPB의 ferbam 솔루션을 준비합니다. 주의 : Ferbam 매우 휘발성이다. 주의 사항 (마스크와 고글) 고체 무게 사용합니다. <ol><li> 2 mg을 ferbam 분말을 달아 원액 (100 PPM)를 만들기 위해 20 ㎖ 50 % 아세토 니트릴 (10 ㎖의 아세토 니트릴과 물 10 ㎖)에 용해. 30 초 동안 플라스크를 소용돌이. </li><li> 시험관에 100 ppm의 ferbam 용액 1 ㎖를 취하여 10 ppm의 용액을 만들기 위해 9 ml의 50 % 아세토 니트릴을 추가한다. 5 초간 튜브를 소용돌이. </li><li> 1 mL를 취하여10 ppm의 시험 관내 용액을 9 ml의 50 % 아세토 니트릴을 추가 1 ppm의 용액을 제조한다. 5 초간 튜브를 소용돌이. </li><li> 시험관에 1 ppm의 용액 1 ㎖를 취하여 100 ppb의 용액을 만들기 위해 9 ml의 50 % 아세토 니트릴을 추가한다. 5 초간 튜브를 소용돌이. </li><li> 시험관에 100 ppb의 용액 1 ㎖를 취하여 10 ppb의 용액을 만들기 위해 9 ml의 50 % 아세토 니트릴을 추가한다. 5 초간 튜브를 소용돌이. </li></ol></li><li> 1 ppm의 암피실린 솔루션을 준비합니다. <ol><li> 10 mg을 암피실린 분말을 달아 100 ppm의 암피실린 용액을 100 ml의 물에 용해. 30 초 동안 플라스크를 소용돌이. </li><li> 시험관에 100 ppm의 용액 1 ㎖를 취하여 10 ppm의 암피실린 용액을 만들기 위해 9 ml의 물을 첨가 하였다. 5 초간 튜브를 소용돌이. </li><li> 시험관에 10 ppm의 용액 1 ㎖를 취하여 1 ppm의 암피실린 용액을 만들기 위해 9 ml의 물을 첨가 하였다. 5 초간 튜브를 소용돌이. </li></ol></li><li> 이 위를 향하도록 NP 코팅면, 필터 홀더에 다시 필터 멤브레인을 넣습니다. &lt;/ 리&gt; <li> 로드 5 새로운 주사기로 하나의 샘플 ml의 다음 내부의 Ag 코팅 멤브레인 필터 홀더에 부착합니다. </li><li> 수동 1 방울 / 초의 유속으로 막을 통해 시료의 전체 볼륨을 통과한다. 대상 분자 여과막 코팅 NP에 흡착하고 농축 될 수있다. </li><li> 약 3 분 동안 필터 홀더, 건조 공기의 필터 멤브레인을 분리하여 단계 2.6에 기재된 방법과 동일한 방법을 사용하여 라만 장비를 사용하여 신호를 측정한다. </li><li> 2.6 단계를 반복 2.2 다른 AG-코팅 막을 제조하고, 다른 시료의 검출을위한 단계 3.3에서 수행합니다. </li></ol>

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E., Goodacre, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+monitoring+of+antibiotics+using+Raman+and+surface+enhanced+Raman+spectroscopy.">Rapid monitoring of antibiotics using Raman and surface enhanced Raman spectroscopy.</a> Analyst. 130 (7), 1019-1026 (2005).</li><li>Zheng, J., Pang, S., Labuza, T. P., He, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Semi-quantification+of+surface-enhanced+Raman+scattering+using+a+handheld+Raman+spectrometer:+a+feasibility+study.">Semi-quantification of surface-enhanced Raman scattering using a handheld Raman spectrometer: a feasibility study.</a> Analyst. 138 (23), 7075-7078 (2013).</li><li>Zheng, J., Pang, S., Labuza, T. P., He, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Evaluation+of+surface-enhanced+Raman+scattering+detection+using+a+handheld+and+a+bench-top+Raman+spectrometer:+A+comparative+study.">Evaluation of surface-enhanced Raman scattering detection using a handheld and a bench-top Raman spectrometer: A comparative study.</a> Talanta. 129, 79-85 (2014).</li></ol>

The authors have nothing to disclose.

이 프로토콜의 중요한 단계 중 하나는 유니폼의 Ag NPS에서 일관된 결과의 핵심의 Ag NP에 합성이다. 가열 시간 및 전구체의 농도를 정밀하게 제어되어야한다. 이 AGNPS 제제의 평균 크기를 측정 하였다 제타는 80nm이다 (데이터는 보이지 않음). 다른 중요한 단계는 염 농도 응집 시간을 정확하게 제어해야 염 집합이다. 작은 기공 크기를 갖는 멤브레인 트랩의 Ag 나노 클러스터로 더 효과적 발견되었을?...

표면 강화 라만 분광 (SERS)는 나노 기술에 라만 분광법을 조합 한 기술이다. 노블 금속 나노 표면에서의 분석 물질의 라만 산란 강도는 크게 국부 표면 플라스 몬 공명에 의해 강화된다. 한 나노 입자은 (Ag NPS에서) 지금까지 의해 가장 널리 사용되는 SERS 높아 확장 능력 기판. 최대 2 아르 ),은 (Ag NP는 다양한 합성 방법이 개발되었다. 3-6의 Ag NPS는 감도 및 / 또는 기능을 향상시키는 것이 효과적 SERS 기판으로 단독으로 사용하거나 다른 재료 및 구조와 결합 할 수있다. 7-11 SERS 기술은 식품 및 환경 시료에 다양한 미량 오염 물질의 검출을위한 훌륭한 능력을 증명하고있다 (12) 전통적으로, SERS 샘플 준비하기위한 일반적인 두 가지 방법이 있습니다 :.. 솔루션 기반 및 기판 기반의 방법 (13)을 솔루션 기반의 운전 방식d를 샘플로 혼합 NP 콜로이드를 사용합니다. 이어서 NP-분석 착체 원심 분리하여 수집하고, 건조 후의 라만 측정 용 고체 지지체 상에 증착된다. 기판 기반 방법은 일반적으로 예비 제작 된 고체 기판 상에 액체 시료 몇 ㎕를 증착에 의해 도포된다. (14) 그러나, 이들 두 가지 방법 중 어느 쪽의 샘플 볼륨 다량 효과적이고 적용 가능하다. SERS 분석의 여러 변형은 필터 시스템 15-21 통합 또는 미세 유동 장치의 혼입 부피 제한을 극복. 21-24 변성 SERS 분석법은 화학 오염 물질을 모니터링하기위한 감도 및 타당성에 큰 개선을 보여 주었다 큰 물 샘플에서. 여기서 우리는 농약 ferbam 항생제 암피실린 미량 검출하도록 제조 및 주사기 필터 SERS 기반 방법의 적용에 대한 상세한 프로토콜을 보여준다.

<table><tbody><tr><td>Ampicillin</td><td>Fisher Scientific</td><td>BP1760-5</td><td>N/A</td></tr><tr><td>Ferbam</td><td>Chem Service</td><td>N-11970-250MG</td><td>98+%</td></tr><tr><td>Silver nitrate</td><td>Sigma Aldrich</td><td>209139</td><td>99.0+%</td></tr><tr><td>Sodium citrate dehydrate</td><td>Sigma Aldrich</td><td>W302600</td><td>99+%</td></tr><tr><td>Sodium chloride</td><td>Sigma Aldrich</td><td>S7653</td><td>99.5+%</td></tr><tr><td>EMD Millipore Durapore PVDF Membrane Filters</td><td>Fisher Scientific</td><td>VVLP01300</td><td>0.10 &amp;micro;m Pore Size, hydrophilic</td></tr><tr><td>Polycarbonate Filter Holders</td><td>Cole-Parmer</td><td>EW-29550-40</td><td>13 mm diameter</td></tr><tr><td>Analog Vortex Mixer</td><td>Fisher Scientific</td><td>02-215-365</td><td>N/A</td></tr><tr><td>Nutating Mixers</td><td>Fisher Scientific</td><td>05-450-213</td><td>N/A</td></tr><tr><td>DXR Raman spectroscope</td><td>Thermo Scientific</td><td>IQLAADGABFFAHCMAPB</td><td>Laser power: 1 mW&lt;br /&gt; Exposure time: 5 sec</td></tr></tbody></table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

이 실험의 주요 단계는 개략도 (도 1)에 도시 하였다.도 2는 최대 감도를 달성하기 위해, 막 코팅 AGNPS의 최적 체적을 사용하는 중요성을 보여주고있다. ferbam을 사용하는 경우 0.5 ㎖ (불충분 한 코팅) 2 ㎖ (너무 코팅)에 비해의 Ag NP에 1 ㎖, 가장 강한 신호를 제공한다. 우리가 개발 한 필터 SE...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

제조 및 화학적 오염 물질 (예를 들어, 농약 ferbam 항생제 암피실린)의 검출을 위해 라만 분광 (SERS) 분석 향상된 필터 기반의 표면을 행하는 방법이 제시된다.

화학 오염 물질의 신속 검출을위한 라만 분광 분석 향상된 필터 기반의 표면

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

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Research

JoVE Journal

Chemistry

9.3K 조회수.  University of Massachusetts Amherst.  제조 및 화학적 오염 물질 (예를 들어, 농약 ferbam 항생제 암피실린)의 검출을 위해 라만 분광 (SERS) 분석 향상된 필터 기반의 표면을 행하는 방법이 제시된다. 

비디오: A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, a large infrastructure of immunoassay instruments and materials can be found. For example, 96- and 384-well polystyrene plates are available commercially and have a standard design to accommodate ultraviolet-visible (UV-Vis) spectroscopy machines from various manufacturers. In addition, a wide variety of immunoglobulins, detection tags, and blocking agents for customized immunoassay designs such as enzyme-linked immunosorbent assays (ELISA) are available.
Despite the existing infrastructure, standard ELISA kits do not meet all research needs, requiring individualized immunoassay development, which can be expensive and time-consuming. For example, ELISA kits have low multiplexing (detection of more than one analyte at a time) capabilities as they usually depend on fluorescence or colorimetric methods for detection. Colorimetric and fluorescent-based analyses have limited multiplexing capabilities due to broad spectral peaks. In contrast, Raman spectroscopy-based methods have a much greater capability for multiplexing due to narrow emission peaks. Another advantage of Raman spectroscopy is that Raman reporters experience significantly less photobleaching than fluorescent tags1. Despite the advantages that Raman reporters have over fluorescent and colorimetric tags, protocols to fabricate Raman-based immunoassays are limited. The purpose of this paper is to provide a protocol to prepare functionalized probes to use in conjunction with polystyrene plates for direct detection of analytes by UV-Vis analysis and Raman spectroscopy. This protocol will allow researchers to take a do-it-yourself approach for future multi-analyte detection while capitalizing on pre-established infrastructure.

Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

자외선 VI와 라만 분광학 면역 플랫폼 제조

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, ...

연구

생화학

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

표면 EBL 날조 된 나노 구조 기판을 사용하여 생체 분자의 라만 분광학 감지 강화

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules ...

공학

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods. The high throughput capabilities of the direct analysis in real time-high resolution mass spectrometry protocol, coupled with multivariate statistical analysis processing of the data, provide not only class characterization of plants, but also yield species and varietal information. Here, the technique is demonstrated with two psychoactive plant products, Mitragyna speciosa (Kratom) and Datura (Jimsonweed), which were subjected to direct analysis in real time-high resolution mass spectrometry followed by statistical analysis processing of the mass spectral data. The application of these tools in tandem enabled the plant materials to be rapidly identified at the level of variety and species.

A method for species identification of botanical material by direct analysis in real time-high resolution mass spectrometry and multivariate statistical analysis is presented.

실시간 고해상도 질량 분석에 직접 분석을 사용하여 식물 소재의 신속한 높은 처리량 종 확인

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, ...

화학

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, when used together, can indicate the presence of gunshot residue (GSR) by producing a fluorescence signal in the presence of three key analytes (antimony, lead, and organic components of GSR). The protocol describes the synthesis of the biosensors using Escherichia coli (E. coli), and the analytical chemistry methods used to evaluate the selectivity and sensitivity of the sensors. The functioning of the system is demonstrated by using GSR collected from the inside of a spent cartridge casing. Once prepared, the biosensors can be stored until needed and can be used as a test for these key analytes. A positive response from all three analytes provides a presumptive positive test for GSR, while each individual device has applications for detecting the analytes in other samples (e.g., a detector for lead contamination in drinking water). The main limitation of the system is the time required for a positive signal; future work may involve studying different organisms to optimize the response time.

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

총 탄 잔류물의 추정 검출을 위한 새로운 세균 바이오 센서의 제조 및 적용

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, ...

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps at high speed, and high spatial resolution of thin samples by directly interrogating their molecular vibrations. In its standard implementation, SRS microscopy is narrowband and forms images with only a single vibrational frequency at a time. However, this approach not only hinders the chemical specificity of SRS but also neglects the wealth of information encoded within vibrational spectra. 
These limitations can be overcome by broadband SRS, an implementation capable of extracting a vibrational spectrum per pixel of the image in parallel. This delivers hyperspectral data that, when coupled with chemometric analysis, maximizes the amount of information retrieved from the specimen. Thus, broadband SRS improves the chemical specificity of the system, allowing the quantitative determination of the concentration of the different constituents of a sample. Here, we report a protocol for chemical imaging with broadband SRS microscopy, based on a home-built SRS microscope operating with a custom differential multichannel-lock-in amplifier detection. It discusses the sample preparation, alignment of the SRS apparatus, and chemometric analysis. By acquiring vibrational Raman spectra, the protocol illustrates how to identify different chemical species within a mixture, determining their relative concentrations.

We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

광대역 자극 라만 산란 현미경을 기반으로 한 멀티플렉스 화학 이미징

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps ...

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging technologies. Hyperspectral SRS and CARS imaging offer Raman spectral information at every pixel, which enables better separation of different chemical compositions. Although both techniques require two excitation lasers, their signal detection schemes and spectral properties are quite different. The goal of this protocol is to perform both hyperspectral SRS and CARS imaging on a single platform and compare the two microscopy techniques for imaging different biological samples. The spectral focusing method is employed to acquire spectral information using femtosecond lasers. By using standard chemical samples, the sensitivity, spatial resolution, and spectral resolution of SRS and CARS in the same excitation conditions (i.e., power at the sample, pixel dwell time, objective lens, pulse energy) are compared. The imaging contrasts of CARS and SRS for biological samples are juxtaposed and compared. The direct comparison of CARS and SRS performances would allow for optimal selection of the modality for chemical imaging.

This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.

화학 이미징을 위한 하이퍼스펙트럼 자극 라만 산란 및 일관된 안티스토크스 라만 산란 현미경의 직접 비교

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging ...

Laser Ablation을 통한 나노입자와 나노구조체 합성

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields such as sensing, catalysis, and medicine. The exceptional feature of this technique is the formation of nanoparticles (colloids) and nanostructures (solids) in a single experiment with ultrashort laser pulses. We have been working on this technique for the past few years, investigating its potential using the surface-enhanced Raman scattering (SERS) technique in hazardous materials sensing applications. Ultrafast laser-ablated substrates (solids and colloids) could detect several analyte molecules at the trace levels/mixture form, including dyes, explosives, pesticides, and biomolecules. Here, we present some of the results achieved using the targets of Ag, Au, Ag-Au, and Si. We have optimized the nanostructures (NSs) and nanoparticles (NPs) obtained (in liquids and air) using different pulse durations, wavelengths, energies, pulse shapes, and writing geometries. Thus, various NSs and NPs were tested for their efficiency in sensing numerous analyte molecules using a simple, portable Raman spectrometer. This methodology, once optimized, paves the way for on-field sensing applications. We discuss the protocols in (a) synthesizing the NPs/NSs via laser ablation, (b) characterization of NPs/NSs, and (c) their utilization in the SERS-based sensing studies.

저자 스포트라이트: Advancements and Applications in Nanoparticle Synthesis Through Laser Ablation in Liquids 

Ultrafast laser ablation in liquid is a precise and versatile technique for synthesizing nanomaterials (nanoparticles [NPs] and nanostructures [NSs]) in liquid/air environments. The laser-ablated nanomaterials can be functionalized with Raman-active molecules to enhance the Raman signal of analytes placed on or near the NSs/NPs.

표면 강화 라만 산란 기반 감지 응용 분야를 위한 초고속 레이저 절제 나노 입자 및 나노 구조

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields ...

R6G 및 Thiram의 SERS 검출을 위한 유연한 기판 제작

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

This article presents a fabrication method for a flexible substrate designed for Surface-Enhanced Raman Scattering (SERS). Silver nanoparticles (AgNPs) were synthesized through a complexation reaction involving silver nitrate (AgNO3) and ammonia, followed by reduction using glucose. The resulting AgNPs exhibited a uniform size distribution ranging from 20 nm to 50 nm. Subsequently, 3-aminopropyl triethoxysilane (APTES) was employed to modify a PDMS substrate that had been surface-treated with oxygen plasma. This process facilitated the self-assembly of AgNPs onto the substrate. A systematic evaluation of the impact of various experimental conditions on substrate performance led to the development of a SERS substrate with excellent performance and an Enhanced Factor (EF). Utilizing this substrate, impressive detection limits of 10-10 M for R6G (Rhodamine 6G) and 10-8 M for Thiram were achieved. The substrate was successfully employed for detecting pesticide residues on apples, yielding highly satisfactory results. The flexible SERS substrate demonstrates great potential for real-world applications, including detection in complex scenarios.

저자 스포트라이트: Synthesized AgNPs를 사용한 SERS Flexible Substrates의 개발 및 응용. 

This protocol describes a fabrication method for a flexible substrate for surface-enhanced Raman scattering. This method has been used in the successful detection of low concentrations of R6G and Thiram.

초고감도 검출을 위한 폴리디메틸실록산(PDMS) 기반 SERS(Flexible Surface-Enhanced Raman Scattering) 기판 제작

This article presents a fabrication method for a flexible substrate designed for Surface-Enhanced Raman Scattering (SERS). Silver nanoparticles (AgNPs) ...

화학 오염 물질의 신속 검출을위한 라만 분광 분석 향상된 필터 기반의 표면

요약

더 많은 비디오 탐색

이 비디오의 챕터

자외선 VI와 라만 분광학 면역 플랫폼 제조

표면 EBL 날조 된 나노 구조 기판을 사용하여 생체 분자의 라만 분광학 감지 강화

실시간 고해상도 질량 분석에 직접 분석을 사용하여 식물 소재의 신속한 높은 처리량 종 확인

총 탄 잔류물의 추정 검출을 위한 새로운 세균 바이오 센서의 제조 및 적용

광대역 자극 라만 산란 현미경을 기반으로 한 멀티플렉스 화학 이미징

화학 이미징을 위한 하이퍼스펙트럼 자극 라만 산란 및 일관된 안티스토크스 라만 산란 현미경의 직접 비교

표면 강화 라만 산란 기반 감지 응용 분야를 위한 초고속 레이저 절제 나노 입자 및 나노 구조

표면 강화 라만 산란 기반 감지 응용 분야를 위한 초고속 레이저 절제 나노 입자 및 나노 구조

표면 강화 라만 산란 기반 감지 응용 분야를 위한 초고속 레이저 절제 나노 입자 및 나노 구조

초고감도 검출을 위한 폴리디메틸실록산(PDMS) 기반 SERS(Flexible Surface-Enhanced Raman Scattering) 기판 제작